BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0102.Seq (558 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 120 1e-28 SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 114 7e-27 SPBC2F12.12c |||conserved eukaryotic protein|Schizosaccharomyces... 28 0.81 SPAC11E3.10 |||VanZ-like family protein|Schizosaccharomyces pomb... 26 3.3 SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces... 25 5.7 SPBC725.16 |res1|sct1|MBF transcription factor complex subunit R... 25 10.0 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 25 10.0 SPBC1604.06c |||CBF/Mak21 family|Schizosaccharomyces pombe|chr 2... 25 10.0 SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 25 10.0 SPAC15A10.05c |mug182||YjeF family protein|Schizosaccharomyces p... 25 10.0 >SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 120 bits (289), Expect = 1e-28 Identities = 56/75 (74%), Positives = 65/75 (86%) Frame = -2 Query: 548 ENAAHTPHKAGSGVNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIM 369 E A P + G +NFSVFYYEILNSPD+AC LAKQAFD+AI+ELD+L+E+SYKDSTLIM Sbjct: 164 ELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLIM 223 Query: 368 QLLRDNLTLWTSDTQ 324 QLLRDNLTLWTSD + Sbjct: 224 QLLRDNLTLWTSDAE 238 Score = 28.3 bits (60), Expect = 0.81 Identities = 11/14 (78%), Positives = 13/14 (92%) Frame = -3 Query: 550 AKMQPTHPIRLGLA 509 A++ PTHPIRLGLA Sbjct: 163 AELAPTHPIRLGLA 176 >SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 114 bits (275), Expect = 7e-27 Identities = 51/69 (73%), Positives = 61/69 (88%) Frame = -2 Query: 530 PHKAGSGVNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDN 351 P + G +NFSVFYYEIL+SP+ AC LAKQ FD+AI+ELD+L+E+SYKDSTLIMQLLRDN Sbjct: 169 PMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISELDSLSEESYKDSTLIMQLLRDN 228 Query: 350 LTLWTSDTQ 324 LTLWTSD + Sbjct: 229 LTLWTSDAE 237 >SPBC2F12.12c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 517 Score = 28.3 bits (60), Expect = 0.81 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = -2 Query: 545 NAAHTPHKAGSGVNFSVFYYEILNS 471 N AH P KA G F+VFY +++ + Sbjct: 412 NEAHPPPKAVQGYRFNVFYPDLIGT 436 >SPAC11E3.10 |||VanZ-like family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 26.2 bits (55), Expect = 3.3 Identities = 13/32 (40%), Positives = 17/32 (53%), Gaps = 1/32 (3%) Frame = -2 Query: 143 FAADLPVPSKIKCCVIFDGFVF-LYLHWIADI 51 FA LPVP K C F F+ L +W+ D+ Sbjct: 31 FAPALPVPINDKVCHFFVFFLLTLVFYWVFDL 62 >SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces pombe|chr 1|||Manual Length = 369 Score = 25.4 bits (53), Expect = 5.7 Identities = 14/42 (33%), Positives = 21/42 (50%) Frame = +3 Query: 81 YKTVKDDTTFNFRRNGKVRCERLLTFLQATARVGFGDDTAPD 206 ++T D +F + G++R RL+TF GFG PD Sbjct: 237 FETTDADLKEHFGQVGQIRRVRLMTFEDTGKCKGFGFVDFPD 278 >SPBC725.16 |res1|sct1|MBF transcription factor complex subunit Res1|Schizosaccharomyces pombe|chr 2|||Manual Length = 637 Score = 24.6 bits (51), Expect = 10.0 Identities = 12/39 (30%), Positives = 17/39 (43%) Frame = +3 Query: 120 RNGKVRCERLLTFLQATARVGFGDDTAPDDVLECYSFSY 236 RNG +C RLL A++ + P D L S+ Sbjct: 367 RNGAKKCARLLLSFYASSSIPNNQGQYPTDFLSSKDMSF 405 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 24.6 bits (51), Expect = 10.0 Identities = 12/35 (34%), Positives = 21/35 (60%) Frame = +1 Query: 28 IYIHQVSSISAIQCKYRNTKPSKMTQHLILDGTGR 132 I + Q ++I+A+ +YR +K T +ILD G+ Sbjct: 156 IEMKQFTNIAAV-LRYRGVHSAKKTAFIILDNKGK 189 >SPBC1604.06c |||CBF/Mak21 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 485 Score = 24.6 bits (51), Expect = 10.0 Identities = 15/35 (42%), Positives = 17/35 (48%), Gaps = 1/35 (2%) Frame = +3 Query: 30 LYSSGQL-DICNPV*IQKYKTVKDDTTFNFRRNGK 131 L SS QL DIC + Y DD F F +N K Sbjct: 133 LLSSSQLSDICIKDFVTSYLVPYDDVRFFFYKNSK 167 >SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 817 Score = 24.6 bits (51), Expect = 10.0 Identities = 12/47 (25%), Positives = 24/47 (51%) Frame = -2 Query: 491 YYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDN 351 Y ++ D QLA Q F +++ L +++ S ++Q ++DN Sbjct: 449 YDDLFPYMDNFIQLAVQLFYHISKKVNCLYVEAFSSSDAVLQRIQDN 495 >SPAC15A10.05c |mug182||YjeF family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 242 Score = 24.6 bits (51), Expect = 10.0 Identities = 11/35 (31%), Positives = 19/35 (54%) Frame = +1 Query: 40 QVSSISAIQCKYRNTKPSKMTQHLILDGTGRSAAN 144 +++ +S Q YR PS+ +Q LI G G + + Sbjct: 31 ELAGLSVSQAVYREYPPSQYSQVLICCGPGNNGGD 65 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,049,513 Number of Sequences: 5004 Number of extensions: 37614 Number of successful extensions: 109 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 106 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 109 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 233995432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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