BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0100.Seq (598 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 155 4e-39 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 155 4e-39 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 155 4e-39 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 55 9e-09 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.073 SPBC3H7.02 |||sulfate transporter |Schizosaccharomyces pombe|chr... 25 6.3 SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 6.3 SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 25 8.4 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 155 bits (377), Expect = 4e-39 Identities = 73/112 (65%), Positives = 86/112 (76%) Frame = -2 Query: 591 KTTQPKGAADFXAQVIVL*PSWSNLKRFTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 412 K P G A F AQVI+L ++PVLDCHTAHIACKFAE+ EK+DRR+GK E Sbjct: 328 KNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEE 387 Query: 411 NPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 256 +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV Sbjct: 388 SPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 155 bits (377), Expect = 4e-39 Identities = 73/112 (65%), Positives = 86/112 (76%) Frame = -2 Query: 591 KTTQPKGAADFXAQVIVL*PSWSNLKRFTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 412 K P G A F AQVI+L ++PVLDCHTAHIACKFAE+ EK+DRR+GK E Sbjct: 328 KNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEE 387 Query: 411 NPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 256 +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV Sbjct: 388 SPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 155 bits (377), Expect = 4e-39 Identities = 73/112 (65%), Positives = 86/112 (76%) Frame = -2 Query: 591 KTTQPKGAADFXAQVIVL*PSWSNLKRFTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 412 K P G A F AQVI+L ++PVLDCHTAHIACKFAE+ EK+DRR+GK E Sbjct: 328 KNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEE 387 Query: 411 NPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 256 +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV Sbjct: 388 SPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 54.8 bits (126), Expect = 9e-09 Identities = 31/99 (31%), Positives = 51/99 (51%) Frame = -2 Query: 561 FXAQVIVL*PSWSNLKRFTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDA 382 F AQ+ +L ++ V+ HTA FA++ K+D+ T + ++ P G Sbjct: 560 FIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMK 618 Query: 381 AIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 265 I L P+C+E F+++ +GRF +RD TVAVG + Sbjct: 619 IIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKV 657 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 31.9 bits (69), Expect = 0.073 Identities = 15/31 (48%), Positives = 20/31 (64%) Frame = -2 Query: 354 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 262 PLC+ +E P LGRF +R TVA G++K Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589 >SPBC3H7.02 |||sulfate transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 877 Score = 25.4 bits (53), Expect = 6.3 Identities = 9/23 (39%), Positives = 14/23 (60%) Frame = +1 Query: 487 VAIQDWCEPFEI*PGWLKHNDLS 555 +++ ++ EP PGWLK N S Sbjct: 84 ISVGEYVEPLPSVPGWLKQNIFS 106 >SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 268 Score = 25.4 bits (53), Expect = 6.3 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = +2 Query: 377 MAASPDLMDFGLTSVDLPVRRSTFS 451 ++ +PDL D L+SVD P++ +T S Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51 >SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 972 Score = 25.0 bits (52), Expect = 8.4 Identities = 13/32 (40%), Positives = 14/32 (43%) Frame = -3 Query: 131 GVSKEKRATNSFLFYIFYKACNVTLFYNLYKV 36 G S E S+ IFY FYNL KV Sbjct: 762 GASSEMECYFSYCSLIFYYQATTLQFYNLPKV 793 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,448,815 Number of Sequences: 5004 Number of extensions: 47906 Number of successful extensions: 146 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 134 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 139 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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