Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0100.Seq
(598 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 141 3e-34
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 141 3e-34
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 141 3e-34
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 141 3e-34
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 124 5e-29
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 61 6e-10
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 49 2e-06
At4g27900.2 68417.m04005 expressed protein 32 0.33
At4g27900.1 68417.m04004 expressed protein 32 0.33
At3g30560.1 68416.m03867 hypothetical protein 30 1.0
At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing prote... 28 4.1
At5g42860.1 68418.m05224 expressed protein 25 5.0
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 141 bits (342), Expect = 3e-34
Identities = 66/115 (57%), Positives = 85/115 (73%)
Frame = -2
Query: 591 KTTQPKGAADFXAQVIVL*PSWSNLKRFTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 412
K KGAA+F +QVI++ + PVLDCHT+HIA KF+EI K+DRR+GK E
Sbjct: 318 KDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEK 377
Query: 411 NPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVTSR 247
PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V +
Sbjct: 378 EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVDKK 432
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 141 bits (342), Expect = 3e-34
Identities = 66/115 (57%), Positives = 85/115 (73%)
Frame = -2
Query: 591 KTTQPKGAADFXAQVIVL*PSWSNLKRFTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 412
K KGAA+F +QVI++ + PVLDCHT+HIA KF+EI K+DRR+GK E
Sbjct: 318 KDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEK 377
Query: 411 NPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVTSR 247
PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V +
Sbjct: 378 EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVDKK 432
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 141 bits (342), Expect = 3e-34
Identities = 66/115 (57%), Positives = 85/115 (73%)
Frame = -2
Query: 591 KTTQPKGAADFXAQVIVL*PSWSNLKRFTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 412
K KGAA+F +QVI++ + PVLDCHT+HIA KF+EI K+DRR+GK E
Sbjct: 318 KDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEK 377
Query: 411 NPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVTSR 247
PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V +
Sbjct: 378 EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVDKK 432
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 141 bits (342), Expect = 3e-34
Identities = 66/115 (57%), Positives = 85/115 (73%)
Frame = -2
Query: 591 KTTQPKGAADFXAQVIVL*PSWSNLKRFTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 412
K KGAA+F +QVI++ + PVLDCHT+HIA KF+EI K+DRR+GK E
Sbjct: 318 KDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEK 377
Query: 411 NPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVTSR 247
PK +K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V +
Sbjct: 378 EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVDKK 432
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 124 bits (299), Expect = 5e-29
Identities = 53/88 (60%), Positives = 70/88 (79%)
Frame = -2
Query: 510 FTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQ 331
+TPVLDCHT+HIA KF+EI K+D RTG E PK +K+ +AAI+N+ P+KP+ VE++
Sbjct: 11 YTPVLDCHTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTPTKPMVVEAYS 70
Query: 330 EFPPLGRFAVRDMRQTVAVGVIKAVTSR 247
+PPLGRFA+RDMRQTV VGVIK+V +
Sbjct: 71 AYPPLGRFAIRDMRQTVGVGVIKSVVKK 98
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 60.9 bits (141), Expect = 6e-10
Identities = 26/87 (29%), Positives = 47/87 (54%)
Frame = -2
Query: 510 FTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQ 331
+ +L H C+ E+K ++D +T K + +K+G A + + + +C+E F
Sbjct: 441 YKAILHIHAVVEECEIIELKSQIDLKTRKPMKKKVLFVKNGAAVVCRIQVTNSICIEKFS 500
Query: 330 EFPPLGRFAVRDMRQTVAVGVIKAVTS 250
+FP LGRF +R +T+AVG + + S
Sbjct: 501 DFPQLGRFTLRTEGKTIAVGKVTELLS 527
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 49.2 bits (112), Expect = 2e-06
Identities = 23/78 (29%), Positives = 44/78 (56%)
Frame = -2
Query: 489 HTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 310
H A A ++ +D +TG+ T+ +P+ + + +A++ + P+CVE+F E LGR
Sbjct: 586 HHAKEAATVVKLVAMLDPKTGQPTKKSPRCLTAKQSAMLEVSLQNPVCVETFSESRALGR 645
Query: 309 FAVRDMRQTVAVGVIKAV 256
+R +TVA+G + +
Sbjct: 646 VFLRSSGRTVAMGKVTRI 663
>At4g27900.2 68417.m04005 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.33
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -2
Query: 408 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 316
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g27900.1 68417.m04004 expressed protein
Length = 261
Score = 31.9 bits (69), Expect = 0.33
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -2
Query: 408 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 316
PKS+ SG+ + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At3g30560.1 68416.m03867 hypothetical protein
Length = 1473
Score = 30.3 bits (65), Expect = 1.0
Identities = 16/49 (32%), Positives = 22/49 (44%)
Frame = -1
Query: 589 NNPT*GCCRFXSSSHCALTILVKSQTVHTSLGLPHCPHCLQICRNQRKS 443
NN C +SS A +L +T H+ G+P PH C +R S
Sbjct: 1041 NNKGGDICLNVASSGIASLLLEGGRTAHSRFGIPLTPHETSTCNMERGS 1089
>At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing protein
similar to cell death suppressor protein lls1 from Zea
mays [gi:1935909], Rieske iron-sulfur protein Tic55 from
Pisum sativum [gi:2764524]; contains Pfam PF00355 Rieske
[2Fe-2S] domain
Length = 536
Score = 28.3 bits (60), Expect = 4.1
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = -2
Query: 468 KFAEIKEKVDRRTGKSTEVNPKSI 397
+F + KEK+DR GK E+N K +
Sbjct: 258 RFPKPKEKIDREGGKPLEINVKKL 281
>At5g42860.1 68418.m05224 expressed protein
Length = 320
Score = 25.0 bits (52), Expect(2) = 5.0
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = -3
Query: 215 RKGHQGQEVARAVNSTIFHTTAILHSPKGVSKEKRATNS 99
R H G++ A T FH+T +L SP G +++S
Sbjct: 33 RDSHDGEKTA-----TSFHSTPVLTSPMGSPPHSHSSSS 66
Score = 21.4 bits (43), Expect(2) = 5.0
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -3
Query: 359 PSLYV*SPSRNSH 321
P+ +V SPSR+SH
Sbjct: 24 PAYFVQSPSRDSH 36
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,920,900
Number of Sequences: 28952
Number of extensions: 257079
Number of successful extensions: 703
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 681
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 695
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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