BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0099.Seq (479 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) simi... 123 7e-29 At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB) 116 8e-27 At3g52640.1 68416.m05799 nicastrin-related contains weak similar... 30 0.70 At5g45490.1 68418.m05588 disease resistance protein-related cont... 29 1.6 At1g12710.1 68414.m01474 F-box family protein / SKP1 interacting... 27 5.0 At3g52640.2 68416.m05800 nicastrin-related contains weak similar... 27 8.7 >At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) similar to 40S ribosomal protein S3A (S phase specific protein GBIS289) GB:P49396 [Brassica rapa] Length = 262 Score = 123 bits (296), Expect = 7e-29 Identities = 61/83 (73%), Positives = 68/83 (81%) Frame = +2 Query: 2 FTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDAERSF 181 F++KDWYDVKAPS+F+ R VG TLV+RTQGTKIASEGLK RVFEVSLADLQ D D ++ Sbjct: 24 FSKKDWYDVKAPSIFTHRNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQGDED--NAY 81 Query: 182 RKFRLIAEYVQGRNVLCNXHGMD 250 RK RL AE VQGRNVLC GMD Sbjct: 82 RKIRLRAEDVQGRNVLCQFWGMD 104 Score = 95.9 bits (228), Expect = 1e-20 Identities = 41/74 (55%), Positives = 58/74 (78%) Frame = +1 Query: 256 TDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGXTNKDSLSQRKTCYAQHTQVRAIRK 435 TDKLR +VKKWQTLIEA++DVKTTD Y LR+FCI T + + ++TCYAQ +Q+R IR+ Sbjct: 107 TDKLRSLVKKWQTLIEAHVDVKTTDSYTLRLFCIAFTKRRANQVKRTCYAQSSQIRQIRR 166 Query: 436 KMCEIITRDVTNSE 477 KM +I+ R+ ++ + Sbjct: 167 KMRDIMVREASSCD 180 >At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB) Length = 262 Score = 116 bits (279), Expect = 8e-27 Identities = 59/83 (71%), Positives = 65/83 (78%) Frame = +2 Query: 2 FTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDAERSF 181 F++KDWYDVKAP F+ R VG TLV+RTQGTKIASEGLK RVFEVSLADLQ D D ++ Sbjct: 24 FSKKDWYDVKAPGSFTNRNVGKTLVSRTQGTKIASEGLKHRVFEVSLADLQNDED--NAY 81 Query: 182 RKFRLIAEYVQGRNVLCNXHGMD 250 RK RL AE VQGRNVL GMD Sbjct: 82 RKIRLRAEDVQGRNVLTQFWGMD 104 Score = 99.1 bits (236), Expect = 1e-21 Identities = 42/74 (56%), Positives = 59/74 (79%) Frame = +1 Query: 256 TDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCIGXTNKDSLSQRKTCYAQHTQVRAIRK 435 TDKLR +VKKWQTLIEA++DVKTTDGY LR+FCI T + + ++TCYAQ +Q+R IR+ Sbjct: 107 TDKLRSLVKKWQTLIEAHVDVKTTDGYTLRMFCIAFTKRRANQVKRTCYAQSSQIRQIRR 166 Query: 436 KMCEIITRDVTNSE 477 KM EI+ ++ ++ + Sbjct: 167 KMSEIMVKEASSCD 180 >At3g52640.1 68416.m05799 nicastrin-related contains weak similarity to Nicastrin precursor (Swiss-Prot:Q92542) [Homo sapiens] Length = 676 Score = 30.3 bits (65), Expect = 0.70 Identities = 19/58 (32%), Positives = 32/58 (55%), Gaps = 3/58 (5%) Frame = -3 Query: 453 NDFTH-FLSDCSDLSVLGVARLA--LAQGVLIGXTNAEDT*NVSIGCLHINVGFDESL 289 N F H L D S+++ V A +A+ + I ++ +DT N ++G +H+N F E L Sbjct: 430 NKFYHSHLDDLSNINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHVNASFVEEL 487 >At5g45490.1 68418.m05588 disease resistance protein-related contains Pfam domain, PF00931: NB-ARC domain, a novel signalling motif found in plant resistance gene products Length = 354 Score = 29.1 bits (62), Expect = 1.6 Identities = 22/80 (27%), Positives = 33/80 (41%), Gaps = 4/80 (5%) Frame = +2 Query: 5 TRKDWYDVK--APSMFSKRQVGTTLVNRTQG-TKIASEGLKGRVFEV-SLADLQADTDAE 172 T KDW DV A K++ R + +K+ + G E+ SL D Sbjct: 22 TCKDWLDVNGLAKGNLEKKRDDNEEEERLKTESKLPGHDIHGFDNEIKSLQHFLLDQKVR 81 Query: 173 RSFRKFRLIAEYVQGRNVLC 232 R F+ ++ EY G+ LC Sbjct: 82 REFKSLVIVGEYGVGKTALC 101 >At1g12710.1 68414.m01474 F-box family protein / SKP1 interacting partner 3-related contains Pfam profile PF00646: F-box domain Length = 291 Score = 27.5 bits (58), Expect = 5.0 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 3/37 (8%) Frame = +2 Query: 215 GRNVLCNX---HGMDLKPISSGGWLKNGRLSSKPTLM 316 GR V CN HG D+KP+ W ++G+ SS ++ Sbjct: 195 GRRV-CNTEQVHGWDIKPVRFQLWTEDGQYSSSQCML 230 >At3g52640.2 68416.m05800 nicastrin-related contains weak similarity to Nicastrin precursor (Swiss-Prot:Q92542) [Homo sapiens] Length = 705 Score = 26.6 bits (56), Expect = 8.7 Identities = 14/42 (33%), Positives = 26/42 (61%) Frame = -3 Query: 414 SVLGVARLALAQGVLIGXTNAEDT*NVSIGCLHINVGFDESL 289 SV+ A + +A+ + I ++ +DT N ++G +H+N F E L Sbjct: 476 SVVAAASV-VARTLYILASDNKDTSNSALGSIHVNASFVEEL 516 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,073,389 Number of Sequences: 28952 Number of extensions: 194661 Number of successful extensions: 541 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 529 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 539 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 819227264 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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