Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0090.Seq
(499 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 109 3e-25
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 109 3e-25
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 109 3e-25
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 43 2e-05
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.055
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 25 6.3
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 25 6.3
SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|... 25 8.4
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 8.4
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 109 bits (261), Expect = 3e-25
Identities = 51/77 (66%), Positives = 60/77 (77%)
Frame = -2
Query: 492 EKVXRRTGKSTEVNPKSIKSGDAAIVXLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG 313
EK+ RR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVG
Sbjct: 375 EKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVG 434
Query: 312 VIKAVNFKEAGGGKVTK 262
VIKAV G KVTK
Sbjct: 435 VIKAVEKVAPGAAKVTK 451
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 109 bits (261), Expect = 3e-25
Identities = 51/77 (66%), Positives = 60/77 (77%)
Frame = -2
Query: 492 EKVXRRTGKSTEVNPKSIKSGDAAIVXLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG 313
EK+ RR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVG
Sbjct: 375 EKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVG 434
Query: 312 VIKAVNFKEAGGGKVTK 262
VIKAV G KVTK
Sbjct: 435 VIKAVEKVAPGAAKVTK 451
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 109 bits (261), Expect = 3e-25
Identities = 51/77 (66%), Positives = 60/77 (77%)
Frame = -2
Query: 492 EKVXRRTGKSTEVNPKSIKSGDAAIVXLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG 313
EK+ RR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVG
Sbjct: 375 EKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVG 434
Query: 312 VIKAVNFKEAGGGKVTK 262
VIKAV G KVTK
Sbjct: 435 VIKAVEKVAPGAAKVTK 451
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 43.2 bits (97), Expect = 2e-05
Identities = 21/62 (33%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
Frame = -2
Query: 477 RTGKSTEVNPKSIKSGDAAIVXLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKA 301
+T + ++ P G I L P+C+E F+++ +GRF +RD TVAVG V+K
Sbjct: 601 KTNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKI 660
Query: 300 VN 295
++
Sbjct: 661 LD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.055
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = -2
Query: 396 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 304
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 972
Score = 25.0 bits (52), Expect = 6.3
Identities = 13/32 (40%), Positives = 14/32 (43%)
Frame = -3
Query: 173 GVSKEKRATNSFLFYIFYKACNVTLFYNLYKV 78
G S E S+ IFY FYNL KV
Sbjct: 762 GASSEMECYFSYCSLIFYYQATTLQFYNLPKV 793
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 25.0 bits (52), Expect = 6.3
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +2
Query: 266 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 388
+ L P +S + TP+AT S+ + P GG D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284
>SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 885
Score = 24.6 bits (51), Expect = 8.4
Identities = 11/35 (31%), Positives = 20/35 (57%)
Frame = -3
Query: 239 QEVARAVNSTIFHTTAILHSPKGVSKEKRATNSFL 135
+ +A A+N +I TT + K + E+ ++ SFL
Sbjct: 369 EALAYAINPSILPTTLLTSYQKSIQDEENSSVSFL 403
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 24.6 bits (51), Expect = 8.4
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +2
Query: 419 MAASPDLMDFGLTSVDLPVRRXTFS 493
++ +PDL D L+SVD P++ T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,825,253
Number of Sequences: 5004
Number of extensions: 32862
Number of successful extensions: 95
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 93
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 95
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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