Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0077.Seq
(648 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 174 8e-45
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 102 4e-23
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 101 9e-23
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 100 4e-22
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 70 3e-13
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 65 1e-11
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 58 8e-10
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 47 3e-06
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 36 0.005
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.082
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 0.44
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.1
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 4.1
SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 26 5.4
SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr 1|||Ma... 26 5.4
SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 25 7.1
SPBC2F12.11c |rep2||transcriptional activator Rep2|Schizosacchar... 25 9.4
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 174 bits (424), Expect = 8e-45
Identities = 78/85 (91%), Positives = 81/85 (95%)
Frame = +2
Query: 254 QYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETF 433
+YPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQI+FETF
Sbjct: 68 KYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETF 127
Query: 434 NTPAMYVAIQAVLSLYASGRTTGIV 508
N PA YVAIQAVLSLYASGRTTGIV
Sbjct: 128 NAPAFYVAIQAVLSLYASGRTTGIV 152
Score = 138 bits (333), Expect = 8e-34
Identities = 62/69 (89%), Positives = 66/69 (95%)
Frame = +3
Query: 57 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 236
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 237 RGILTLNTP 263
RGILTL P
Sbjct: 62 RGILTLKYP 70
Score = 58.8 bits (136), Expect = 6e-10
Identities = 28/47 (59%), Positives = 31/47 (65%)
Frame = +1
Query: 508 VDSGDGVFHTVPIYEGYALPYAIXXXXXXXXXXXXXXMKILTERGYS 648
+DSGDGV HTVPIYEGYALP+AI MKIL ERGY+
Sbjct: 153 LDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYT 199
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 102 bits (245), Expect = 4e-23
Identities = 42/85 (49%), Positives = 65/85 (76%)
Frame = +2
Query: 254 QYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETF 433
+YP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + MFE +
Sbjct: 68 KYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERY 127
Query: 434 NTPAMYVAIQAVLSLYASGRTTGIV 508
+YVAIQAVLSLYA G ++G+V
Sbjct: 128 GFGGVYVAIQAVLSLYAQGLSSGVV 152
Score = 60.1 bits (139), Expect = 3e-10
Identities = 32/69 (46%), Positives = 43/69 (62%), Gaps = 2/69 (2%)
Frame = +3
Query: 63 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSK 236
E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61
Query: 237 RGILTLNTP 263
R +L + P
Sbjct: 62 RSLLQVKYP 70
Score = 36.3 bits (80), Expect = 0.004
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +1
Query: 508 VDSGDGVFHTVPIYEGYALPYAIXXXXXXXXXXXXXXMKILTERGYS 648
VDSGDGV H VP+YE L + + + +L +GY+
Sbjct: 153 VDSGDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYA 199
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 101 bits (242), Expect = 9e-23
Identities = 46/86 (53%), Positives = 61/86 (70%), Gaps = 1/86 (1%)
Frame = +2
Query: 254 QYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIMFET 430
Q PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + +ET
Sbjct: 72 QRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYET 131
Query: 431 FNTPAMYVAIQAVLSLYASGRTTGIV 508
N PA+ ++Q VL+LYAS RTTGIV
Sbjct: 132 LNVPALSFSLQPVLALYASARTTGIV 157
Score = 78.6 bits (185), Expect = 7e-16
Identities = 34/63 (53%), Positives = 45/63 (71%)
Frame = +3
Query: 75 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 254
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L +
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71
Query: 255 NTP 263
P
Sbjct: 72 QRP 74
Score = 35.1 bits (77), Expect = 0.009
Identities = 12/23 (52%), Positives = 20/23 (86%)
Frame = +1
Query: 508 VDSGDGVFHTVPIYEGYALPYAI 576
++ GDG+ H+VPIY+G+++P AI
Sbjct: 158 LECGDGLTHSVPIYDGFSIPSAI 180
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 99.5 bits (237), Expect = 4e-22
Identities = 42/77 (54%), Positives = 53/77 (68%)
Frame = +2
Query: 257 YPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN 436
YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE+FN
Sbjct: 92 YPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFN 151
Query: 437 TPAMYVAIQAVLSLYAS 487
+Y+A+QAVL+L AS
Sbjct: 152 CAGLYIAVQAVLALAAS 168
Score = 42.7 bits (96), Expect = 4e-05
Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +3
Query: 75 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 224
+++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ +
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58
Score = 33.5 bits (73), Expect = 0.027
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +1
Query: 508 VDSGDGVFHTVPIYEGYALPYAI 576
VDSGDGV H +P+ EGY + +I
Sbjct: 184 VDSGDGVTHIIPVAEGYVIGSSI 206
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 70.1 bits (164), Expect = 3e-13
Identities = 40/108 (37%), Positives = 55/108 (50%), Gaps = 2/108 (1%)
Frame = +2
Query: 182 DGRH--GTERLVRRRRGTEQKRYPDPQYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 355
DGR G E + + G E K I +G V NWD +W + +L+ P EH
Sbjct: 46 DGRRLFGEEYIYKSNPGMEIKN------AIRNGWVENWDVTVDLWRYGLEQQLKTNPLEH 99
Query: 356 PVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTT 499
P+L+TE NP NR K + MFE+ PA Y+A Q + +ASG+ T
Sbjct: 100 PILITEPFDNPPENRVKTLETMFESLRCPATYLAKQETCAAFASGKGT 147
Score = 40.3 bits (90), Expect = 2e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +3
Query: 60 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 161
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 64.9 bits (151), Expect = 1e-11
Identities = 32/97 (32%), Positives = 54/97 (55%), Gaps = 2/97 (2%)
Frame = +2
Query: 260 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 439
P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N
Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144
Query: 440 PAMYVAIQAVLSLYASGR--TTGIVWTPATVSSTPYP 544
P++ I + S Y + + ++GIV +S P
Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIP 181
Score = 35.9 bits (79), Expect = 0.005
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +3
Query: 75 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 170
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 58.4 bits (135), Expect = 8e-10
Identities = 23/80 (28%), Positives = 47/80 (58%)
Frame = +2
Query: 269 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 448
+GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA
Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133
Query: 449 YVAIQAVLSLYASGRTTGIV 508
Y+ QAV +A+ ++T ++
Sbjct: 134 YLTKQAVCVAFANSKSTALI 153
Score = 38.7 bits (86), Expect = 7e-04
Identities = 20/57 (35%), Positives = 33/57 (57%)
Frame = +3
Query: 60 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 230
EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 46.8 bits (106), Expect = 3e-06
Identities = 28/92 (30%), Positives = 43/92 (46%), Gaps = 4/92 (4%)
Frame = +2
Query: 245 PDPQYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMT 412
P+ PI+ G V +W+ ++ W H Y+ L P + +PV L +RE T
Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELAT 166
Query: 413 QIMFETFNTPAMYVAIQAVLSLYASGRTTGIV 508
Q FE P +A + ++ LYA G G+V
Sbjct: 167 QFFFEECQVPGFTIAYEPLMGLYAIGILHGLV 198
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 35.9 bits (79), Expect = 0.005
Identities = 19/55 (34%), Positives = 29/55 (52%)
Frame = +3
Query: 120 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLNTPSNTESSL 284
G+ PRA F ++ P H G+++ M KD G+E S +G + TP N S +
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTPENEISEV 554
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.9 bits (69), Expect = 0.082
Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +2
Query: 320 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 463
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 29.5 bits (63), Expect = 0.44
Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%)
Frame = -3
Query: 217 PTYESFCPM-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 56
PT S P+ PT+ P P N S P PL+TTN TS+S
Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 4.1
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 362 LLTEAPLNPKANREKMTQIMFETFN 436
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 4.1
Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Frame = -3
Query: 220 SPTYESFC--PMPTITP*WRGLPTIEGNTARGASSPAKPAL 104
S T +SF P+PT P LPT NT + P+ PAL
Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101
>SPCP1E11.04c |pal1||membrane associated protein Pal1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 25.8 bits (54), Expect = 5.4
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 114 NRPCTYRSHCLLPTRQLP 61
NRP YRSH + TR+ P
Sbjct: 323 NRPSNYRSHSSMGTRRSP 340
>SPAP27G11.03 |||D123 family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 319
Score = 25.8 bits (54), Expect = 5.4
Identities = 9/34 (26%), Positives = 19/34 (55%)
Frame = +2
Query: 248 DPQYPIEHGIVTNWDDMEKIWHHTFYNELRVAPE 349
+P YP G++ +W ++E + E+R+ P+
Sbjct: 241 NPFYPRTDGLLFSWSELESMNSENMKPEIRLIPK 274
>SPBC23G7.08c |rga7||GTPase activating protein
Rga7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 25.4 bits (53), Expect = 7.1
Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Frame = -3
Query: 244 IPLLLCASSPTYESFCPMPTI-TP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAA 68
IP L ++P +F P P++ +P + T +TA ++SP L P + TN +
Sbjct: 363 IPSPLQNTNPAPSTF-PNPSVASPAFPNSSTSNPSTAPASASPLASTLK-PSTANDTNGS 420
Query: 67 TSSS 56
+SSS
Sbjct: 421 SSSS 424
>SPBC2F12.11c |rep2||transcriptional activator
Rep2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 219
Score = 25.0 bits (52), Expect = 9.4
Identities = 16/49 (32%), Positives = 24/49 (48%)
Frame = +2
Query: 434 NTPAMYVAIQAVLSLYASGRTTGIVWTPATVSSTPYPSTRDTHFLTPSC 580
N P Y +LS A+ IV +P++VSS + S+ D F+ C
Sbjct: 14 NNPPTYPWSSPILSSIANPSLCDIVSSPSSVSS--FASSDDFAFMNAYC 60
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,779,682
Number of Sequences: 5004
Number of extensions: 61279
Number of successful extensions: 212
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 207
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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