Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0071.Seq
(518 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g18080.1 68414.m02238 WD-40 repeat family protein / auxin-dep... 56 1e-08
At1g48630.1 68414.m05440 guanine nucleotide-binding family prote... 56 2e-08
At3g18130.1 68416.m02305 guanine nucleotide-binding family prote... 55 3e-08
At1g11160.1 68414.m01278 WD-40 repeat family protein / katanin p... 29 1.9
At5g08390.1 68418.m00988 transducin family protein / WD-40 repea... 29 2.5
At3g10530.1 68416.m01264 transducin family protein / WD-40 repea... 28 4.3
At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p... 28 4.3
At3g59590.1 68416.m06649 jacalin lectin family protein similar t... 27 5.7
At5g10940.1 68418.m01269 transducin family protein / WD-40 repea... 27 7.6
>At1g18080.1 68414.m02238 WD-40 repeat family protein /
auxin-dependent protein (ARCA) / guanine
nucleotide-binding protein beta subunit, putative
identical to SP|O24456 Guanine nucleotide-binding
protein beta subunit-like protein (WD-40 repeat
auxin-dependent protein ARCA) {Arabidopsis thaliana};
contains 7 WD-40 repeats (PF00400)
Length = 327
Score = 56.4 bits (130), Expect = 1e-08
Identities = 41/121 (33%), Positives = 57/121 (47%), Gaps = 10/121 (8%)
Frame = -3
Query: 516 GSLGHLNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLHTLDRNXIITXLXLLXXQXLXX 337
G G+++T SPDGSLCASGGK + WDL +G L++L+ N +I L +
Sbjct: 192 GHTGYVSTVAVSPDGSLCASGGKDGVVLLWDLAEGKKLYSLEANSVIHALCFSPNR--YW 249
Query: 336 XCLRTFH----QDLGSGKQXDG*R------AQA*NH*QTXTSKTXPPQCLSLAWSTDGQT 187
C T H DL S + + A+ ++ +K C SL WS DG T
Sbjct: 250 LCAATEHGIKIWDLESKSIVEDLKVDLKAEAEKADNSGPAATKRKVIYCTSLNWSADGST 309
Query: 186 L 184
L
Sbjct: 310 L 310
Score = 39.5 bits (88), Expect = 0.001
Identities = 18/37 (48%), Positives = 23/37 (62%)
Frame = -1
Query: 371 ALXFSXXRXWXXXAXGPSIKIWDLXSKXMVEELRXEI 261
AL FS R W A IKIWDL SK +VE+L+ ++
Sbjct: 240 ALCFSPNRYWLCAATEHGIKIWDLESKSIVEDLKVDL 276
>At1g48630.1 68414.m05440 guanine nucleotide-binding family protein
/ activated protein kinase C receptor, putative / RACK,
putative contains 7 WD-40 repeats (PF00400); very
similar to guanine nucleotide-binding protein; activated
protein kinase C receptor; RACK1 (GI:9294068)
{Arabidopsis thaliana}; similar to WD-40 repeat
auxin-dependent protein ARCA (SP:O24456) [Arabidopsis
thaliana];
Length = 326
Score = 55.6 bits (128), Expect = 2e-08
Identities = 43/121 (35%), Positives = 56/121 (46%), Gaps = 10/121 (8%)
Frame = -3
Query: 516 GSLGHLNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLHTLDRNXIITXLXLLXXQXLXX 337
G G+LNT SPDGSLCASGGK + WDL +G L++L+ II L +
Sbjct: 191 GHSGYLNTVAVSPDGSLCASGGKDGVILLWDLAEGKKLYSLEAGSIIHSLCFSPNR--YW 248
Query: 336 XCLRTFHQ----DLGSGKQXDG*R------AQA*NH*QTXTSKTXPPQCLSLAWSTDGQT 187
C T + DL S + + A+ + +KT C SL WS DG T
Sbjct: 249 LCAATENSIRIWDLESKSVVEDLKVDLKAEAEKTDGSTGIGNKTKVIYCTSLNWSADGNT 308
Query: 186 L 184
L
Sbjct: 309 L 309
Score = 38.7 bits (86), Expect = 0.002
Identities = 17/37 (45%), Positives = 24/37 (64%)
Frame = -1
Query: 371 ALXFSXXRXWXXXAXGPSIKIWDLXSKXMVEELRXEI 261
+L FS R W A SI+IWDL SK +VE+L+ ++
Sbjct: 239 SLCFSPNRYWLCAATENSIRIWDLESKSVVEDLKVDL 275
>At3g18130.1 68416.m02305 guanine nucleotide-binding family protein
/ activated protein kinase C receptor (RACK1) identical
to guanine nucleotide-binding protein; activated protein
kinase C receptor; RACK1 (GI:9294068) {Arabidopsis
thaliana}; contains Pfam profile: PF00400 WD domain,
G-beta repeat (7 copies)
Length = 326
Score = 54.8 bits (126), Expect = 3e-08
Identities = 26/50 (52%), Positives = 32/50 (64%)
Frame = -3
Query: 516 GSLGHLNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLHTLDRNXIITXL 367
G G+LNT SPDGSLCASGGK + WDL +G L++L+ II L
Sbjct: 191 GHSGYLNTVAVSPDGSLCASGGKDGVILLWDLAEGKKLYSLEAGSIIHSL 240
Score = 38.7 bits (86), Expect = 0.002
Identities = 17/37 (45%), Positives = 24/37 (64%)
Frame = -1
Query: 371 ALXFSXXRXWXXXAXGPSIKIWDLXSKXMVEELRXEI 261
+L FS R W A SI+IWDL SK +VE+L+ ++
Sbjct: 239 SLCFSPNRYWLCAATENSIRIWDLESKSVVEDLKVDL 275
>At1g11160.1 68414.m01278 WD-40 repeat family protein / katanin p80
subunit, putative similar to contains 6 WD-40 repeats
(PF00400); katanin p80 subunit (GI:3005601)
[Strongylocentrotus purpuratus]
Length = 974
Score = 29.1 bits (62), Expect = 1.9
Identities = 14/34 (41%), Positives = 16/34 (47%)
Frame = -3
Query: 501 LNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLH 400
++T SPDG SGG WDL G LH
Sbjct: 94 ISTIEFSPDGRWVVSGGLDNVVKVWDLTAGKLLH 127
>At5g08390.1 68418.m00988 transducin family protein / WD-40 repeat
family protein similar to katanin p80 subunit
[Strongylocentrotus purpuratus] GI:3005601; contains
Pfam profile PF00400: WD domain, G-beta repeat
Length = 871
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = -3
Query: 501 LNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLH 400
+N +PDG SGG+ WDL G LH
Sbjct: 239 VNVLRFTPDGRWIVSGGEDNVVKVWDLTAGKLLH 272
>At3g10530.1 68416.m01264 transducin family protein / WD-40 repeat
family protein contains 2 WD-40 repeats (PF00400); BING4
(gi:3811380) {Mus musculus]; similar to hypothetical
protein GB:P40055 [Saccharomyces cerevisiae]
Length = 536
Score = 27.9 bits (59), Expect = 4.3
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = -3
Query: 507 GHLNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLHTL 394
G +++ P+G L A+ GK K WDL + T+
Sbjct: 282 GPVSSVAFHPNGHLMATSGKERKIKIWDLRKFEEVQTI 319
>At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p80
subunit, putative contains 5 WD-40 repeats (PF00400);
similar to katanin p80 subunit (GI:3005601)
[Strongylocentrotus purpuratus]
Length = 1180
Score = 27.9 bits (59), Expect = 4.3
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = -3
Query: 501 LNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLH 400
++T +PDG SGG WDL G LH
Sbjct: 145 ISTIRFTPDGRWVVSGGLDNVVKVWDLTAGKLLH 178
>At3g59590.1 68416.m06649 jacalin lectin family protein similar to
suppressor of nim1-1 [Arabidopsis thaliana] GI:22023774;
contains Pfam profile PF01419: Jacalin-like lectin
domain
Length = 454
Score = 27.5 bits (58), Expect = 5.7
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -3
Query: 507 GHLNTXTXSPDGSLCASGGKYMKAM 433
GH N T +PD + +GG Y++A+
Sbjct: 350 GHGNATTHNPDDEIMIAGGDYIEAV 374
>At5g10940.1 68418.m01269 transducin family protein / WD-40 repeat
family protein unnamed ORF cDNA FLJ10872, Homo sapiens,
EMBL:AK001734; contains Pfam PF00400: WD domain, G-beta
repeat (6 copies,1 weak)
Length = 757
Score = 27.1 bits (57), Expect = 7.6
Identities = 12/42 (28%), Positives = 21/42 (50%)
Frame = -3
Query: 516 GSLGHLNTXTXSPDGSLCASGGKYMKAMXWDLNDGXHLHTLD 391
G G +N + +GSL SG ++ W+ + LH++D
Sbjct: 48 GHQGCVNALAWNSNGSLLISGSDDLRINIWNYSSRKLLHSID 89
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,564,339
Number of Sequences: 28952
Number of extensions: 124804
Number of successful extensions: 363
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 323
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 363
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 947539968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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