Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0040.Seq
(347 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) riboso... 131 1e-31
At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S ri... 126 5e-30
At2g02950.1 68415.m00242 phytochrome kinase substrate 1 (PKS1) i... 27 3.4
At5g37210.1 68418.m04468 DC1 domain-containing protein contains ... 26 6.0
At4g19030.1 68417.m02804 major intrinsic family protein / MIP fa... 26 6.0
At2g21650.1 68415.m02575 myb family transcription factor contain... 26 6.0
At1g32400.2 68414.m03998 senescence-associated family protein co... 26 6.0
At1g32400.1 68414.m03997 senescence-associated family protein co... 26 6.0
>At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) ribosomal
protein S8 - Zea mays, PIR:T04088
Length = 222
Score = 131 bits (317), Expect = 1e-31
Identities = 64/114 (56%), Positives = 78/114 (68%), Gaps = 1/114 (0%)
Frame = +3
Query: 3 RDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQR-IHSVRSRGGNTKYRALRLDTG 179
RD HKRRATGGK+ RKKRKYE+GR ANT+L + + +R RGGN K+RALRLDTG
Sbjct: 5 RDSIHKRRATGGKQKQWRKKRKYEMGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLDTG 64
Query: 180 NFSWGSECSTRKTRIIDVVYNASNNNWCVQRPLSRMQLX**MQLHSGSWYESHY 341
N+SWGSE +TRKTR++DVVYNASNN + L + + WY SHY
Sbjct: 65 NYSWGSEATTRKTRVLDVVYNASNNELVRTKTLVKSAIVQVDAAPFKQWYLSHY 118
>At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S
ribosomal protein S8, Prunus armeniaca, EMBL:AF071889
Length = 210
Score = 126 bits (303), Expect = 5e-30
Identities = 60/85 (70%), Positives = 67/85 (78%), Gaps = 1/85 (1%)
Frame = +3
Query: 3 RDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQR-IHSVRSRGGNTKYRALRLDTG 179
RD HKRRATGGK+ RKKRKYELGR ANT+L + + +R RGGN K+RALRLDTG
Sbjct: 5 RDSIHKRRATGGKQKMWRKKRKYELGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLDTG 64
Query: 180 NFSWGSECSTRKTRIIDVVYNASNN 254
NFSWGSE TRKTRI+DV YNASNN
Sbjct: 65 NFSWGSEAVTRKTRILDVAYNASNN 89
Score = 35.1 bits (77), Expect = 0.013
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = +2
Query: 257 LVRTKTLVKNAIXVVDATPFRQ 322
LVRT+TLVK+AI VDA PF+Q
Sbjct: 91 LVRTQTLVKSAIVQVDAAPFKQ 112
>At2g02950.1 68415.m00242 phytochrome kinase substrate 1 (PKS1)
identical to Swiss-Prot:Q9SWI1 phytochrome kinase
substrate 1 [Arabidopsis thaliana]
Length = 439
Score = 27.1 bits (57), Expect = 3.4
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 45 APIRKKRKYELGRPAANTRLGPQRIHSVRSRGGN 146
+P+R+ R ++ R + PQR S S GGN
Sbjct: 339 SPVRRNRPTQIPRIPITAKSAPQRRKSSSSSGGN 372
>At5g37210.1 68418.m04468 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 625
Score = 26.2 bits (55), Expect = 6.0
Identities = 15/54 (27%), Positives = 23/54 (42%)
Frame = -3
Query: 213 CELNIPIPKRSYRCPDAAHGT*YFHHVNERSGYAEDRAWCLQRDALTHTSSSCV 52
CE I KR Y C + T + + + Y + +W L + L T S C+
Sbjct: 568 CEGEIDPKKRFYMCDEYCCVTLHINCMLGADKYMDPGSWWLYKGNLDGTCSGCM 621
>At4g19030.1 68417.m02804 major intrinsic family protein / MIP
family protein contains Pfam profile: MIP PF00230;
identical to cDNA NLM1 protein GI:2677613
Length = 296
Score = 26.2 bits (55), Expect = 6.0
Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Frame = +1
Query: 85 PLQTPGSVLSVSTPFVH--VVEILSTVRCVWTPVTSL 189
PL+ S+LSVS PF+ + E L T V+T S+
Sbjct: 39 PLKKQDSLLSVSVPFLQKLIAEFLGTYFLVFTGCASV 75
>At2g21650.1 68415.m02575 myb family transcription factor contains
PFAM profile: PF00249 myb-like DNA-binding domain
Length = 101
Score = 26.2 bits (55), Expect = 6.0
Identities = 13/25 (52%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
Frame = +3
Query: 6 DHWHK-RRATGGKRAPIRKKRKYEL 77
D WH RA GGK P KR+Y+L
Sbjct: 35 DRWHNVARAVGGK-TPEEAKRQYDL 58
>At1g32400.2 68414.m03998 senescence-associated family protein
contains Pfam profile PF00335: Tetraspanin family
Length = 280
Score = 26.2 bits (55), Expect = 6.0
Identities = 10/29 (34%), Positives = 14/29 (48%)
Frame = +3
Query: 186 SWGSECSTRKTRIIDVVYNASNNNWCVQR 272
SW E + +T D +YN NW + R
Sbjct: 137 SWRDELPSDRTGNFDTIYNFLRENWKIVR 165
>At1g32400.1 68414.m03997 senescence-associated family protein
contains Pfam profile PF00335: Tetraspanin family
Length = 280
Score = 26.2 bits (55), Expect = 6.0
Identities = 10/29 (34%), Positives = 14/29 (48%)
Frame = +3
Query: 186 SWGSECSTRKTRIIDVVYNASNNNWCVQR 272
SW E + +T D +YN NW + R
Sbjct: 137 SWRDELPSDRTGNFDTIYNFLRENWKIVR 165
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,554,831
Number of Sequences: 28952
Number of extensions: 180066
Number of successful extensions: 418
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 409
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 415
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 429398688
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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