Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0030.Seq
(329 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl... 29 0.75
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl... 29 0.75
At3g06670.1 68416.m00786 expressed protein 28 1.7
At5g39210.1 68418.m04749 expressed protein 26 5.3
At5g18530.1 68418.m02191 beige/BEACH domain-containing protein c... 26 5.3
At5g39460.1 68418.m04779 F-box family protein contains Pfam:PF00... 26 7.0
At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical ... 26 7.0
At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical ... 26 7.0
At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical ... 26 7.0
At2g40980.1 68415.m05062 expressed protein 26 7.0
>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 490
Score = 29.1 bits (62), Expect = 0.75
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +1
Query: 136 GVLKTCSDDNVALCLKEKALRYVENVSXSXELN-LIDGVSLS 258
GV+ C DDN ++ + E LR+ E + E + +ID V +S
Sbjct: 159 GVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEIS 200
>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 561
Score = 29.1 bits (62), Expect = 0.75
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +1
Query: 136 GVLKTCSDDNVALCLKEKALRYVENVSXSXELN-LIDGVSLS 258
GV+ C DDN ++ + E LR+ E + E + +ID V +S
Sbjct: 159 GVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEIS 200
>At3g06670.1 68416.m00786 expressed protein
Length = 865
Score = 27.9 bits (59), Expect = 1.7
Identities = 12/21 (57%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Frame = +1
Query: 109 DEDVFRSVMGVLKTCSD-DNV 168
D D FR++MGV K C D +NV
Sbjct: 167 DHDFFRNLMGVFKICEDLENV 187
>At5g39210.1 68418.m04749 expressed protein
Length = 156
Score = 26.2 bits (55), Expect = 5.3
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -1
Query: 227 SXEXETFSTYRKAFSLRHNATLSSEQVFST 138
S + + F+ K FS RHN +S EQV T
Sbjct: 4 SLQKQLFNNGDKLFSSRHNRRVSIEQVHVT 33
>At5g18530.1 68418.m02191 beige/BEACH domain-containing protein
contains 5 WD-40 repeats (PF00400); contains
Beige/BEACH domain (Pfam PF02138); FACTOR ASSOCIATED
WITH N-SMASE ACTIVATION (FAN) (SP:Q92636) Homo
sapiens;similar to Lipopolysaccharide-responsive and
beige-like anchor protein (CDC4-like protein)
(Beige-like protein) (SP:P50851) [Homo sapiens}
Length = 909
Score = 26.2 bits (55), Expect = 5.3
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -2
Query: 145 SALPSLIGRRLRHYSLRPQRILLTPPGASW 56
SAL L G ++ H +RP ILL+ SW
Sbjct: 270 SALAHLHGLKVSHGDIRPSNILLSDSLWSW 299
>At5g39460.1 68418.m04779 F-box family protein contains Pfam:PF00646
F-box domain ; similar to SKP1 interacting partner 2
(SKIP2) TIGR_Ath1:At5g67250
Length = 571
Score = 25.8 bits (54), Expect = 7.0
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +1
Query: 208 NVSXSXELNLIDGVSLSVKAXLDQPGPXSLYPT 306
++ S +NL+D V+ V +D+P +L+PT
Sbjct: 206 DLDFSDRMNLLDIVTKHVSLRVDEPLTGNLFPT 238
>At3g53570.3 68416.m05915 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 453
Score = 25.8 bits (54), Expect = 7.0
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -2
Query: 187 FP*DTMRRCHRSKFSALPSLIGRRLRHYSLRPQRILL 77
FP D +R R ++ + RL H L+P+ ILL
Sbjct: 198 FPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILL 234
>At3g53570.2 68416.m05916 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 467
Score = 25.8 bits (54), Expect = 7.0
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -2
Query: 187 FP*DTMRRCHRSKFSALPSLIGRRLRHYSLRPQRILL 77
FP D +R R ++ + RL H L+P+ ILL
Sbjct: 212 FPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILL 248
>At3g53570.1 68416.m05917 protein kinase (AFC1) (AME2) identical to
protein kinase AFC1 (EC 2.7.1.-) [Arabidopsis thaliana]
Length = 467
Score = 25.8 bits (54), Expect = 7.0
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -2
Query: 187 FP*DTMRRCHRSKFSALPSLIGRRLRHYSLRPQRILL 77
FP D +R R ++ + RL H L+P+ ILL
Sbjct: 212 FPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILL 248
>At2g40980.1 68415.m05062 expressed protein
Length = 617
Score = 25.8 bits (54), Expect = 7.0
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = -2
Query: 184 P*DTMRRCHRSKFSALPSLIGRRLRHYSLRPQRILLTPPG 65
P + +R CH SAL S +RH +RP+ ++ G
Sbjct: 445 PEEAIRCCHDC-LSALSSSSSAGIRHGDIRPENVVYVTSG 483
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.312 0.131 0.364
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,555,812
Number of Sequences: 28952
Number of extensions: 105051
Number of successful extensions: 155
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 380568784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.8 bits)
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