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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0022.Seq
         (499 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo...    76   3e-15
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc...    60   2e-10
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc...    58   7e-10
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|...    51   8e-08
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha...    51   1e-07
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos...    41   9e-05
SPBC800.03 |clr3||histone deacetylase |Schizosaccharomyces pombe...    26   3.6  
SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces p...    25   6.3  
SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase Pmp1|...    25   6.3  
SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomy...    25   8.4  

>SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1055

 Score = 76.2 bits (179), Expect = 3e-15
 Identities = 39/73 (53%), Positives = 53/73 (72%), Gaps = 2/73 (2%)
 Frame = -2

Query: 468  FVQYRSXXRAXDVREQLVSLMERVEIEMVTSLSDDTN-IRKAIXSGYFYHIAKFSKGG-H 295
            F+QY+S  RA DVR+QL +L ERVEIE+VT+ S+  + I+KAI +GYF + A+  + G  
Sbjct: 921  FLQYKSLCRARDVRDQLANLCERVEIELVTNSSESLDPIKKAITAGYFSNAARLDRSGDS 980

Query: 294  YKTVKHNQXVMIH 256
            Y+TVK NQ V IH
Sbjct: 981  YRTVKSNQTVYIH 993



 Score = 66.1 bits (154), Expect = 3e-12
 Identities = 26/55 (47%), Positives = 42/55 (76%)
 Frame = -1

Query: 265  YDTSNSALFEDLPRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHYYKSKELED 101
            Y   +S++ E  P+ +IY ELV T+KE+ RQ+TEI+ +WLLE++PHY+K + +E+
Sbjct: 991  YIHPSSSVAEKKPKVIIYFELVLTTKEYCRQITEIQPEWLLEISPHYFKPENIEE 1045


>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
           Prp43|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 735

 Score = 60.1 bits (139), Expect = 2e-10
 Identities = 32/76 (42%), Positives = 49/76 (64%), Gaps = 5/76 (6%)
 Frame = -2

Query: 468 FVQYRSXXRAXDVREQLVSLMERVEIEMVTSLSDD----TNIRKAIXSGYFYHIAKFSKG 301
           F+ +R+   A +VR+QL   MER E+E++++  DD     NIR+A+ SG+F  +AK S  
Sbjct: 585 FLSHRALISADNVRKQLRRTMERQEVELISTPFDDKNYYVNIRRALVSGFFMQVAKKSAN 644

Query: 300 G-HYKTVKHNQXVMIH 256
           G +Y T+K NQ V +H
Sbjct: 645 GKNYVTMKDNQVVSLH 660



 Score = 58.4 bits (135), Expect = 6e-10
 Identities = 25/52 (48%), Positives = 36/52 (69%)
 Frame = -1

Query: 229 PRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHYYKSKELEDSTNKKMPKT 74
           P WV+Y+E V T+K F+R VT I  +WL+E+AP+YY   +L+D  N K  K+
Sbjct: 669 PEWVVYNEFVLTTKSFIRNVTAIRPEWLIELAPNYY---DLDDFDNNKEVKS 717


>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
            Prp22|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1168

 Score = 58.0 bits (134), Expect = 7e-10
 Identities = 29/66 (43%), Positives = 39/66 (59%), Gaps = 1/66 (1%)
 Frame = -1

Query: 265  YDTSNSALFEDLPRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHYYK-SKELEDSTNK 89
            Y   +  LF     WVIYHEL+ TSKE+M  V+ +  KWL+EVAP ++K +   + S  K
Sbjct: 1081 YMHPSGVLFGKAAEWVIYHELIQTSKEYMHTVSTVNPKWLVEVAPTFFKFANANQVSKTK 1140

Query: 88   KMPKTL 71
            K  K L
Sbjct: 1141 KNLKVL 1146



 Score = 51.6 bits (118), Expect = 6e-08
 Identities = 29/72 (40%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
 Frame = -2

Query: 468  FVQYRSXXRAXDVREQLVSLMERVEIEMVTSLSDDTNIRKAIXSGYFYHIAK-FSKGGHY 292
            ++Q R   RA DVR+QL+ LM+R    +V+       I +A+ SGYF ++AK  S  G Y
Sbjct: 1012 YIQARGMRRAEDVRKQLIRLMDRYRHPVVSCGRKRELILRALCSGYFTNVAKRDSHEGCY 1071

Query: 291  KTVKHNQXVMIH 256
            KT+  N  V +H
Sbjct: 1072 KTIVENAPVYMH 1083


>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
            Prp16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1173

 Score = 51.2 bits (117), Expect = 8e-08
 Identities = 23/41 (56%), Positives = 27/41 (65%)
 Frame = -1

Query: 232  LPRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHYYKSKE 110
            LP +VIYHELV TSKE+M  VT ++  WL E    YY  KE
Sbjct: 1072 LPDYVIYHELVLTSKEYMNIVTSVDPYWLAEFGGVYYSVKE 1112



 Score = 43.2 bits (97), Expect = 2e-05
 Identities = 25/75 (33%), Positives = 43/75 (57%), Gaps = 1/75 (1%)
 Frame = -2

Query: 474  KPFVQYRSXXRAXDVREQLVSLMERVEIEMVTSLSDDTNIRKAIXSGYFYHIAKFSKG-G 298
            K F+  ++  RA D+R+QLV +M + +I +  S+SD   +R+ + S YF H A  +KG G
Sbjct: 990  KHFLHSKTLKRARDIRQQLVEIMSKQKISL-ESVSDWDIVRRVLCSAYF-HQAACAKGIG 1047

Query: 297  HYKTVKHNQXVMIHL 253
             Y  ++      +H+
Sbjct: 1048 EYVHLRSGMPCHLHV 1062


>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
           Prh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 719

 Score = 50.8 bits (116), Expect = 1e-07
 Identities = 22/43 (51%), Positives = 30/43 (69%)
 Frame = -1

Query: 253 NSALFEDLPRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHY 125
           +S+LF      ++YHELVFT+K ++R V+ I S WL  VAPHY
Sbjct: 671 SSSLFGKKVEAIMYHELVFTTKSYVRGVSSIRSNWLNAVAPHY 713



 Score = 34.7 bits (76), Expect = 0.008
 Identities = 23/82 (28%), Positives = 39/82 (47%), Gaps = 2/82 (2%)
 Frame = -2

Query: 495 RVQWXLXKPFVQYRSXXRAXDVREQLVS--LMERVEIEMVTSLSDDTNIRKAIXSGYFYH 322
           R QW   + F+  R+     D+R+QL    L +  E+     ++ + N+  +  SGY  +
Sbjct: 590 RKQWC-SQNFINRRALKTILDIRKQLREHCLKDGWELNSSPEVNSE-NLLLSFLSGYITN 647

Query: 321 IAKFSKGGHYKTVKHNQXVMIH 256
            A     G Y+T+  NQ + IH
Sbjct: 648 TALLHPDGSYRTIIGNQTISIH 669


>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 647

 Score = 41.1 bits (92), Expect = 9e-05
 Identities = 25/88 (28%), Positives = 42/88 (47%), Gaps = 2/88 (2%)
 Frame = -2

Query: 492 VQWXLXKPFVQYRSXXRAXDVREQLVSLMERVEIEMVTSL--SDDTNIRKAIXSGYFYHI 319
           +QW   K ++ Y++  +A D+R  LV  + +  I     L  SD + I K +  G+  ++
Sbjct: 523 LQWC-RKNYLNYQTLRQALDIRTHLVRFLNKFSIPTAQRLPSSDCSKILKCLLDGFVRNV 581

Query: 318 AKFSKGGHYKTVKHNQXVMIHLIVLYLK 235
           A     G YKT+   Q  +    VL+ K
Sbjct: 582 AHLQNDGSYKTIGGKQVWLDSSSVLHEK 609


>SPBC800.03 |clr3||histone deacetylase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 687

 Score = 25.8 bits (54), Expect = 3.6
 Identities = 10/16 (62%), Positives = 10/16 (62%)
 Frame = -1

Query: 238 EDLPRWVIYHELVFTS 191
           EDLP W   H LVF S
Sbjct: 618 EDLPTWYYRHSLVFVS 633


>SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1033

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 17/53 (32%), Positives = 30/53 (56%)
 Frame = -1

Query: 235  DLPRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHYYKSKELEDSTNKKMPK 77
            +LP   +   LV  SK+ M+ + EIE+K   ++      +K+ E S+N K+P+
Sbjct: 888  NLPSNQLLAMLVKLSKKIMKCIDEIETK---DIEEELGSNKKTE-SSNSKLPE 936


>SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase
           Pmp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 278

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 11/36 (30%), Positives = 19/36 (52%), Gaps = 2/36 (5%)
 Frame = -2

Query: 303 GGHYKTVKHNQXVMI--HLIVLYLKTFHDG*FTMSL 202
           G HY+  KHN  + +  H+  +++   HD  F + L
Sbjct: 101 GRHYRDSKHNLDIQVFDHIEYVHIHWDHDTQFALEL 136


>SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 370

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 9/31 (29%), Positives = 15/31 (48%)
 Frame = +1

Query: 1   NGESGNFX*IYCNQXFTNFLQISPMFWAFSY 93
           +G  G+    YC Q   + ++  P FW  +Y
Sbjct: 63  DGHGGDRVAKYCRQHLPDIIKSQPSFWKGNY 93


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,798,832
Number of Sequences: 5004
Number of extensions: 31915
Number of successful extensions: 66
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 58
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 63
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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