BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0022.Seq (499 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 76 3e-15 SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 60 2e-10 SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 58 7e-10 SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 51 8e-08 SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 51 1e-07 SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 41 9e-05 SPBC800.03 |clr3||histone deacetylase |Schizosaccharomyces pombe... 26 3.6 SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces p... 25 6.3 SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase Pmp1|... 25 6.3 SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomy... 25 8.4 >SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1055 Score = 76.2 bits (179), Expect = 3e-15 Identities = 39/73 (53%), Positives = 53/73 (72%), Gaps = 2/73 (2%) Frame = -2 Query: 468 FVQYRSXXRAXDVREQLVSLMERVEIEMVTSLSDDTN-IRKAIXSGYFYHIAKFSKGG-H 295 F+QY+S RA DVR+QL +L ERVEIE+VT+ S+ + I+KAI +GYF + A+ + G Sbjct: 921 FLQYKSLCRARDVRDQLANLCERVEIELVTNSSESLDPIKKAITAGYFSNAARLDRSGDS 980 Query: 294 YKTVKHNQXVMIH 256 Y+TVK NQ V IH Sbjct: 981 YRTVKSNQTVYIH 993 Score = 66.1 bits (154), Expect = 3e-12 Identities = 26/55 (47%), Positives = 42/55 (76%) Frame = -1 Query: 265 YDTSNSALFEDLPRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHYYKSKELED 101 Y +S++ E P+ +IY ELV T+KE+ RQ+TEI+ +WLLE++PHY+K + +E+ Sbjct: 991 YIHPSSSVAEKKPKVIIYFELVLTTKEYCRQITEIQPEWLLEISPHYFKPENIEE 1045 >SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 60.1 bits (139), Expect = 2e-10 Identities = 32/76 (42%), Positives = 49/76 (64%), Gaps = 5/76 (6%) Frame = -2 Query: 468 FVQYRSXXRAXDVREQLVSLMERVEIEMVTSLSDD----TNIRKAIXSGYFYHIAKFSKG 301 F+ +R+ A +VR+QL MER E+E++++ DD NIR+A+ SG+F +AK S Sbjct: 585 FLSHRALISADNVRKQLRRTMERQEVELISTPFDDKNYYVNIRRALVSGFFMQVAKKSAN 644 Query: 300 G-HYKTVKHNQXVMIH 256 G +Y T+K NQ V +H Sbjct: 645 GKNYVTMKDNQVVSLH 660 Score = 58.4 bits (135), Expect = 6e-10 Identities = 25/52 (48%), Positives = 36/52 (69%) Frame = -1 Query: 229 PRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHYYKSKELEDSTNKKMPKT 74 P WV+Y+E V T+K F+R VT I +WL+E+AP+YY +L+D N K K+ Sbjct: 669 PEWVVYNEFVLTTKSFIRNVTAIRPEWLIELAPNYY---DLDDFDNNKEVKS 717 >SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosaccharomyces pombe|chr 1|||Manual Length = 1168 Score = 58.0 bits (134), Expect = 7e-10 Identities = 29/66 (43%), Positives = 39/66 (59%), Gaps = 1/66 (1%) Frame = -1 Query: 265 YDTSNSALFEDLPRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHYYK-SKELEDSTNK 89 Y + LF WVIYHEL+ TSKE+M V+ + KWL+EVAP ++K + + S K Sbjct: 1081 YMHPSGVLFGKAAEWVIYHELIQTSKEYMHTVSTVNPKWLVEVAPTFFKFANANQVSKTK 1140 Query: 88 KMPKTL 71 K K L Sbjct: 1141 KNLKVL 1146 Score = 51.6 bits (118), Expect = 6e-08 Identities = 29/72 (40%), Positives = 42/72 (58%), Gaps = 1/72 (1%) Frame = -2 Query: 468 FVQYRSXXRAXDVREQLVSLMERVEIEMVTSLSDDTNIRKAIXSGYFYHIAK-FSKGGHY 292 ++Q R RA DVR+QL+ LM+R +V+ I +A+ SGYF ++AK S G Y Sbjct: 1012 YIQARGMRRAEDVRKQLIRLMDRYRHPVVSCGRKRELILRALCSGYFTNVAKRDSHEGCY 1071 Query: 291 KTVKHNQXVMIH 256 KT+ N V +H Sbjct: 1072 KTIVENAPVYMH 1083 >SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|Schizosaccharomyces pombe|chr 2|||Manual Length = 1173 Score = 51.2 bits (117), Expect = 8e-08 Identities = 23/41 (56%), Positives = 27/41 (65%) Frame = -1 Query: 232 LPRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHYYKSKE 110 LP +VIYHELV TSKE+M VT ++ WL E YY KE Sbjct: 1072 LPDYVIYHELVLTSKEYMNIVTSVDPYWLAEFGGVYYSVKE 1112 Score = 43.2 bits (97), Expect = 2e-05 Identities = 25/75 (33%), Positives = 43/75 (57%), Gaps = 1/75 (1%) Frame = -2 Query: 474 KPFVQYRSXXRAXDVREQLVSLMERVEIEMVTSLSDDTNIRKAIXSGYFYHIAKFSKG-G 298 K F+ ++ RA D+R+QLV +M + +I + S+SD +R+ + S YF H A +KG G Sbjct: 990 KHFLHSKTLKRARDIRQQLVEIMSKQKISL-ESVSDWDIVRRVLCSAYF-HQAACAKGIG 1047 Query: 297 HYKTVKHNQXVMIHL 253 Y ++ +H+ Sbjct: 1048 EYVHLRSGMPCHLHV 1062 >SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 719 Score = 50.8 bits (116), Expect = 1e-07 Identities = 22/43 (51%), Positives = 30/43 (69%) Frame = -1 Query: 253 NSALFEDLPRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHY 125 +S+LF ++YHELVFT+K ++R V+ I S WL VAPHY Sbjct: 671 SSSLFGKKVEAIMYHELVFTTKSYVRGVSSIRSNWLNAVAPHY 713 Score = 34.7 bits (76), Expect = 0.008 Identities = 23/82 (28%), Positives = 39/82 (47%), Gaps = 2/82 (2%) Frame = -2 Query: 495 RVQWXLXKPFVQYRSXXRAXDVREQLVS--LMERVEIEMVTSLSDDTNIRKAIXSGYFYH 322 R QW + F+ R+ D+R+QL L + E+ ++ + N+ + SGY + Sbjct: 590 RKQWC-SQNFINRRALKTILDIRKQLREHCLKDGWELNSSPEVNSE-NLLLSFLSGYITN 647 Query: 321 IAKFSKGGHYKTVKHNQXVMIH 256 A G Y+T+ NQ + IH Sbjct: 648 TALLHPDGSYRTIIGNQTISIH 669 >SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizosaccharomyces pombe|chr 1|||Manual Length = 647 Score = 41.1 bits (92), Expect = 9e-05 Identities = 25/88 (28%), Positives = 42/88 (47%), Gaps = 2/88 (2%) Frame = -2 Query: 492 VQWXLXKPFVQYRSXXRAXDVREQLVSLMERVEIEMVTSL--SDDTNIRKAIXSGYFYHI 319 +QW K ++ Y++ +A D+R LV + + I L SD + I K + G+ ++ Sbjct: 523 LQWC-RKNYLNYQTLRQALDIRTHLVRFLNKFSIPTAQRLPSSDCSKILKCLLDGFVRNV 581 Query: 318 AKFSKGGHYKTVKHNQXVMIHLIVLYLK 235 A G YKT+ Q + VL+ K Sbjct: 582 AHLQNDGSYKTIGGKQVWLDSSSVLHEK 609 >SPBC800.03 |clr3||histone deacetylase |Schizosaccharomyces pombe|chr 2|||Manual Length = 687 Score = 25.8 bits (54), Expect = 3.6 Identities = 10/16 (62%), Positives = 10/16 (62%) Frame = -1 Query: 238 EDLPRWVIYHELVFTS 191 EDLP W H LVF S Sbjct: 618 EDLPTWYYRHSLVFVS 633 >SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1033 Score = 25.0 bits (52), Expect = 6.3 Identities = 17/53 (32%), Positives = 30/53 (56%) Frame = -1 Query: 235 DLPRWVIYHELVFTSKEFMRQVTEIESKWLLEVAPHYYKSKELEDSTNKKMPK 77 +LP + LV SK+ M+ + EIE+K ++ +K+ E S+N K+P+ Sbjct: 888 NLPSNQLLAMLVKLSKKIMKCIDEIETK---DIEEELGSNKKTE-SSNSKLPE 936 >SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase Pmp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 278 Score = 25.0 bits (52), Expect = 6.3 Identities = 11/36 (30%), Positives = 19/36 (52%), Gaps = 2/36 (5%) Frame = -2 Query: 303 GGHYKTVKHNQXVMI--HLIVLYLKTFHDG*FTMSL 202 G HY+ KHN + + H+ +++ HD F + L Sbjct: 101 GRHYRDSKHNLDIQVFDHIEYVHIHWDHDTQFALEL 136 >SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 370 Score = 24.6 bits (51), Expect = 8.4 Identities = 9/31 (29%), Positives = 15/31 (48%) Frame = +1 Query: 1 NGESGNFX*IYCNQXFTNFLQISPMFWAFSY 93 +G G+ YC Q + ++ P FW +Y Sbjct: 63 DGHGGDRVAKYCRQHLPDIIKSQPSFWKGNY 93 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,798,832 Number of Sequences: 5004 Number of extensions: 31915 Number of successful extensions: 66 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 58 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 63 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 196153982 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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