Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0020.Seq
         (370 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g35630.1 68417.m05060 phosphoserine aminotransferase, chlorop...    49   8e-07
At2g17630.1 68415.m02039 phosphoserine aminotransferase, putativ...    48   2e-06
At3g59250.1 68416.m06605 F-box family protein contains F-box dom...    27   2.9  
At1g14790.1 68414.m01768 RNA-dependent RNA polymerase, putative ...    27   5.2  
At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearl...    26   9.0  
At2g45890.1 68415.m05707 expressed protein contains Pfam profile...    26   9.0  

>At4g35630.1 68417.m05060 phosphoserine aminotransferase,
           chloroplast (PSAT) identical to Phosphoserine
           aminotransferase, chloroplast precursor (PSAT)
           (SP:Q96255)[Arabidopsis thaliana]; contains TIGRFAM
           TIGR01364: phosphoserine aminotransferase; contains Pfam
           PF00266: aminotransferase, class V
          Length = 430

 Score = 49.2 bits (112), Expect = 8e-07
 Identities = 22/50 (44%), Positives = 32/50 (64%)
 Frame = +2

Query: 110 KVFNFGAGPAKLPEEVYEIIKNELTNFENSGISLLETSHRSSTYMKLNAK 259
           +VFNF AGPA LPE V    + +L N+  SG+S++E SHR   ++ +  K
Sbjct: 72  RVFNFAAGPATLPENVLLKAQADLYNWRGSGMSVMEMSHRGKEFLSIIQK 121



 Score = 31.9 bits (69), Expect = 0.14
 Identities = 14/31 (45%), Positives = 18/31 (58%)
 Frame = +1

Query: 271 VRNLLDVPDNYKVXXXXXXXXXQFAAVPLNL 363
           +R LL++P  Y V         QFAA+PLNL
Sbjct: 126 LRQLLEIPQEYSVLFLQGGATTQFAALPLNL 156


>At2g17630.1 68415.m02039 phosphoserine aminotransferase, putative
           similar to Phosphoserine aminotransferase, chloroplast
           precursor (PSAT) (SP:Q96255) [Arabidopsis thaliana];
           contains TIGRFAM TIGR01364: phosphoserine
           aminotransferase; contains Pfam PF00266:
           aminotransferase, class V
          Length = 422

 Score = 48.0 bits (109), Expect = 2e-06
 Identities = 21/50 (42%), Positives = 32/50 (64%)
 Frame = +2

Query: 110 KVFNFGAGPAKLPEEVYEIIKNELTNFENSGISLLETSHRSSTYMKLNAK 259
           +V NF AGPA LPE V    +++L N+  SG+S++E SHR   ++ +  K
Sbjct: 62  RVINFAAGPAALPENVLLKAQSDLYNWRGSGMSVMEMSHRGKEFLSIIQK 111



 Score = 31.9 bits (69), Expect = 0.14
 Identities = 14/31 (45%), Positives = 18/31 (58%)
 Frame = +1

Query: 271 VRNLLDVPDNYKVXXXXXXXXXQFAAVPLNL 363
           +R LL++P  Y V         QFAA+PLNL
Sbjct: 116 LRQLLEIPSEYSVLFLQGGATTQFAALPLNL 146


>At3g59250.1 68416.m06605 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 425

 Score = 27.5 bits (58), Expect = 2.9
 Identities = 18/67 (26%), Positives = 27/67 (40%)
 Frame = -1

Query: 220 CFQ*TNSRIFKICQFILNNFIDFFWQFSRSSAKIKHFRHFHLFIYLINSYTTLFYQTTLS 41
           CF+   S    + + +LNN     W  S SS  +K    +    Y  N ++ LF    L 
Sbjct: 145 CFEKLTSGCHVLEELVLNNVYSNVWNRSVSSKTLKRLTLWCTESYDKNPHSVLFDTPNLV 204

Query: 40  FSDVSQY 20
           +   S Y
Sbjct: 205 YLKYSDY 211


>At1g14790.1 68414.m01768 RNA-dependent RNA polymerase, putative
           similar to RNA-directed RNA polymerase GB:CAA09697
           GI:4138282 [Nicotiana tabacum]
          Length = 1107

 Score = 26.6 bits (56), Expect = 5.2
 Identities = 9/20 (45%), Positives = 17/20 (85%)
 Frame = -2

Query: 219 VSNKLIPEFSKFVNSFLIIS 160
           VSN+++  +SK++N+FL +S
Sbjct: 386 VSNRVLRHYSKYINNFLRVS 405


>At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearly
           identical to acyl-activating enzyme 17 [Arabidopsis
           thaliana] GI:29893266; similar to acetyl-CoA synthetase
           [SP|P27095] from Methanothrix soehngenii; contains Pfam
           AMP-binding enzyme domain PF00501; identical to cDNA
           acyl-activating enzyme 17 (At5g23050) GI:29893265
          Length = 721

 Score = 25.8 bits (54), Expect = 9.0
 Identities = 13/45 (28%), Positives = 23/45 (51%)
 Frame = +2

Query: 134 PAKLPEEVYEIIKNELTNFENSGISLLETSHRSSTYMKLNAKFRM 268
           P+  PE++   +  +   F N  ++LL+ S  S    KLN  F++
Sbjct: 642 PSGGPEQLVIAVVFKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKV 686


>At2g45890.1 68415.m05707 expressed protein contains Pfam profile
           PF03759: Domain of unknown function (DUF315)
          Length = 463

 Score = 25.8 bits (54), Expect = 9.0
 Identities = 10/22 (45%), Positives = 14/22 (63%)
 Frame = +1

Query: 238 IHEAKCEIQDVVRNLLDVPDNY 303
           IH+A   I D   N +D+PD+Y
Sbjct: 279 IHKAALAINDSTLNDMDIPDSY 300


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,628,941
Number of Sequences: 28952
Number of extensions: 109805
Number of successful extensions: 271
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 267
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 271
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 487896136
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)