BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0010.Seq
(409 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g32730.1 68415.m04005 26S proteasome regulatory subunit, puta... 36 0.008
At1g04810.1 68414.m00477 26S proteasome regulatory subunit, puta... 36 0.010
At3g42830.1 68416.m04485 ring-box protein Roc1/Rbx1/Hrt1, putati... 33 0.074
At5g44130.1 68418.m05401 fasciclin-like arabinogalactan-protein,... 31 0.39
At5g20570.1 68418.m02442 ring-box protein-related similar to rin... 29 1.2
At2g29000.1 68415.m03527 leucine-rich repeat family protein / pr... 28 2.1
At4g38570.1 68417.m05460 CDP-diacylglycerol--inositol 3-phosphat... 28 2.8
At1g35650.1 68414.m04431 Ulp1 protease family protein PF02902: U... 28 2.8
At5g45560.1 68418.m05595 pleckstrin homology (PH) domain-contain... 27 4.8
At4g02120.1 68417.m00283 CTP synthase, putative / UTP--ammonia l... 27 4.8
At2g47820.1 68415.m05968 expressed protein 27 4.8
At4g02920.2 68417.m00396 expressed protein 27 6.4
At4g02920.1 68417.m00395 expressed protein 27 6.4
At1g68000.1 68414.m07768 CDP-diacylglycerol--inositol 3-phosphat... 27 6.4
At3g28340.1 68416.m03540 galactinol synthase, putative 26 8.5
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 26 8.5
At2g26070.1 68415.m03130 expressed protein 26 8.5
At1g78980.1 68414.m09209 leucine-rich repeat transmembrane prote... 26 8.5
>At2g32730.1 68415.m04005 26S proteasome regulatory subunit, putative
contains similarity to 26S proteasome regulatory subunit
S1 SP:O88761, GI:3288594 from [Rattus norvegicus]
Length = 1004
Score = 36.3 bits (80), Expect = 0.008
Identities = 20/52 (38%), Positives = 31/52 (59%)
Frame = -3
Query: 407 FEILSNPARVMRQQLKNLTIVEGSGFTPLKDITIGGIVMLNHTGEGEQVLVS 252
FEIL NPARV+ Q K + +++ S + P+K + G V+L E E ++S
Sbjct: 911 FEILVNPARVVPAQEKYIKLLDDSRYVPVK-LAPSGFVLLKDLREHEPEVLS 961
>At1g04810.1 68414.m00477 26S proteasome regulatory subunit, putative
contains similarity to 26S proteasome regulatory subunit
S1 SP:O88761, GI:3288594 from [Rattus norvegicus]
Length = 1001
Score = 35.9 bits (79), Expect = 0.010
Identities = 20/52 (38%), Positives = 30/52 (57%)
Frame = -3
Query: 407 FEILSNPARVMRQQLKNLTIVEGSGFTPLKDITIGGIVMLNHTGEGEQVLVS 252
FEIL NPARV+ Q K + ++E S + P+K + G V+L E ++S
Sbjct: 904 FEILVNPARVVPSQEKYIKLMEDSRYVPMK-LAPSGFVLLRDLRPHEPEVLS 954
>At3g42830.1 68416.m04485 ring-box protein Roc1/Rbx1/Hrt1, putative
E3 ubiquitin ligase, SCF complex subunit; contains
similarity to ring-box protein 1 RBX1 GI:4769004 from
[Homo sapiens]
Length = 115
Score = 33.1 bits (72), Expect = 0.074
Identities = 17/52 (32%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = -3
Query: 272 GEQVLVSRLPHSARRQRKRRSLNLQSLLNIWMIDVLLSNCNCHRNRV-NICI 120
GE +S +P S+ + KR L S + +W D+++ NC RN + ++CI
Sbjct: 11 GESSSIS-VPSSSSKNSKRFELKKWSAVALWAWDIVVDNCAICRNHIMDLCI 61
>At5g44130.1 68418.m05401 fasciclin-like arabinogalactan-protein,
putative similar to gi_13377784_gb_AAK20861
Length = 247
Score = 30.7 bits (66), Expect = 0.39
Identities = 21/68 (30%), Positives = 33/68 (48%)
Frame = -3
Query: 365 LKNLTIVEGSGFTPLKDITIGGIVMLNHTGEGEQVLVSRLPHSARRQRKRRSLNLQSLLN 186
L++L V T +GG+ LN TG+G QV VS R SL + L
Sbjct: 115 LEDLLSVSNPVRTQASGRDVGGVYGLNFTGQGNQVNVST---GVVETRLSTSLRQERPLA 171
Query: 185 IWMIDVLL 162
++++D++L
Sbjct: 172 VYVVDMVL 179
>At5g20570.1 68418.m02442 ring-box protein-related similar to
ring-box protein 1 GI:4769004 from [Homo sapiens]
Length = 118
Score = 29.1 bits (62), Expect = 1.2
Identities = 12/41 (29%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = -3
Query: 239 SARRQRKRRSLNLQSLLNIWMIDVLLSNCNCHRNRV-NICI 120
S+ ++ KR + S + +W D+++ NC RN + ++CI
Sbjct: 24 SSNKKAKRFEIKKWSAVALWAWDIVVDNCAICRNHIMDLCI 64
>At2g29000.1 68415.m03527 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 872
Score = 28.3 bits (60), Expect = 2.1
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = -3
Query: 302 GIVMLNHTGEGEQVLVSRLPHSARRQRKRRSLNLQSLLNIWMIDV--LLSNCN 150
GIV H + EQV V L HS+ + K+ ++ LL + ++ L+ CN
Sbjct: 577 GIVYHGHLNDTEQVAVKLLSHSSTQGYKQFKAEVELLLRVHHTNLVNLVGYCN 629
>At4g38570.1 68417.m05460 CDP-diacylglycerol--inositol
3-phosphatidyltransferase, putative /
phosphatidylinositol synthase, putative similar to
phosphatidylinositol synthase (PIS1) - Arabidopsis
thaliana, PID:e1313354 [gi:3367632]
Length = 225
Score = 27.9 bits (59), Expect = 2.8
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = -3
Query: 65 YLRVILSLTKFIVCISNKT 9
Y+RV+L+ F VC SNKT
Sbjct: 21 YMRVLLNCIAFSVCFSNKT 39
>At1g35650.1 68414.m04431 Ulp1 protease family protein PF02902: Ulp1
protease family, C-terminal catalytic domain; similar to
At1g21020, At3g26530, At1g08760, At1g08740, At2g29240
Length = 736
Score = 27.9 bits (59), Expect = 2.8
Identities = 14/69 (20%), Positives = 36/69 (52%)
Frame = +3
Query: 174 NHPDIQKALEVQAPSLPLPSGRMRQPANQDLFSFTGVVQHDDTSNSDIL*RSET*TLDNS 353
++ ++ + L + PSLP P+ + P ++ + F ++ D +N+ I +D++
Sbjct: 506 DNANLSQMLMISEPSLPAPTNDIVDPDDKVIDIFINSIRIDWRANNIIEKHQAVSFIDST 565
Query: 354 *ILQLLSHH 380
+++L H+
Sbjct: 566 FVVELCKHY 574
>At5g45560.1 68418.m05595 pleckstrin homology (PH) domain-containing
protein / lipid-binding START domain-containing protein
weak similarity to SP|P79245 Steroidogenic acute
regulatory protein, mitochondrial precursor (StAR) {Ovis
aries}; contains Pfam profiles PF01852: START domain,
PF00169: PH domain
Length = 719
Score = 27.1 bits (57), Expect = 4.8
Identities = 14/45 (31%), Positives = 22/45 (48%), Gaps = 1/45 (2%)
Frame = +3
Query: 180 PDIQKALEVQAPSLPLPSGRMRQPA-NQDLFSFTGVVQHDDTSNS 311
PD + E + + +PA N DL F+G ++HDD N+
Sbjct: 455 PDSEPEPETSKQDQETDAKKTEEPALNIDLSCFSGNLRHDDNENA 499
>At4g02120.1 68417.m00283 CTP synthase, putative / UTP--ammonia
ligase, putative similar to SP|P17812 CTP synthase (EC
6.3.4.2) (UTP--ammonia ligase) {Homo sapiens}; contains
Pfam profile PF00117: glutamine amidotransferase class-I
Length = 556
Score = 27.1 bits (57), Expect = 4.8
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = -3
Query: 209 LNLQSLLNIWMIDVLLSNCNCHRN 138
LN+ + NIW + +LL N N H +
Sbjct: 242 LNIHDVPNIWHVPLLLRNQNAHHS 265
>At2g47820.1 68415.m05968 expressed protein
Length = 805
Score = 27.1 bits (57), Expect = 4.8
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -3
Query: 233 RRQRKRRSLNLQSLLNIWMIDVLLSNCNCH 144
R+ R RRS+ Q LL+ W LLS + H
Sbjct: 207 RKSRSRRSVQGQKLLSGWRQQELLSRISSH 236
>At4g02920.2 68417.m00396 expressed protein
Length = 419
Score = 26.6 bits (56), Expect = 6.4
Identities = 22/83 (26%), Positives = 36/83 (43%)
Frame = -3
Query: 344 EGSGFTPLKDITIGGIVMLNHTGEGEQVLVSRLPHSARRQRKRRSLNLQSLLNIWMIDVL 165
E FTP K + + L + L S H+ +R+ K R L ++ L+ + +
Sbjct: 230 ESDVFTPAKLKAVTVLAPLKSPEKSR--LKSPRKHNTKRKAKERDLYKRNHLHAYESLLS 287
Query: 164 LSNCNCHRNRVNICIVLNKQCIE 96
L N HR++ + L K C E
Sbjct: 288 LMIGNDHRHKHTTVLSLQKSCGE 310
>At4g02920.1 68417.m00395 expressed protein
Length = 418
Score = 26.6 bits (56), Expect = 6.4
Identities = 22/83 (26%), Positives = 36/83 (43%)
Frame = -3
Query: 344 EGSGFTPLKDITIGGIVMLNHTGEGEQVLVSRLPHSARRQRKRRSLNLQSLLNIWMIDVL 165
E FTP K + + L + L S H+ +R+ K R L ++ L+ + +
Sbjct: 229 ESDVFTPAKLKAVTVLAPLKSPEKSR--LKSPRKHNTKRKAKERDLYKRNHLHAYESLLS 286
Query: 164 LSNCNCHRNRVNICIVLNKQCIE 96
L N HR++ + L K C E
Sbjct: 287 LMIGNDHRHKHTTVLSLQKSCGE 309
>At1g68000.1 68414.m07768 CDP-diacylglycerol--inositol
3-phosphatidyltransferase / phosphatidylinositol
synthase (PIS1) identical to phosphatidylinositol
synthase (PIS1) GB:AJ000539 [gi:3367632]
Length = 227
Score = 26.6 bits (56), Expect = 6.4
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -3
Query: 65 YLRVILSLTKFIVCISNK 12
Y+RV+L+ F VC SNK
Sbjct: 24 YMRVLLNCVAFAVCFSNK 41
>At3g28340.1 68416.m03540 galactinol synthase, putative
Length = 365
Score = 26.2 bits (55), Expect = 8.5
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +1
Query: 247 NRLTRTCSPSPVWFNMTIPPIVISFKGVKPEPSTIVKFFNC 369
NRL C PS V MT+ P + +G +I+K +C
Sbjct: 60 NRLLLACDPSAVHIAMTLDPAYL--RGTVSAVHSILKHTSC 98
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 26.2 bits (55), Expect = 8.5
Identities = 9/35 (25%), Positives = 19/35 (54%)
Frame = -3
Query: 380 VMRQQLKNLTIVEGSGFTPLKDITIGGIVMLNHTG 276
+ +L +L ++ G G TP+ ++ G +V + G
Sbjct: 476 IQEAELHSLYLMMGQGLTPVTEVKAGNVVAIRGLG 510
>At2g26070.1 68415.m03130 expressed protein
Length = 250
Score = 26.2 bits (55), Expect = 8.5
Identities = 15/53 (28%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = +1
Query: 247 NRLTRTCSPSPVWFNMTIPPIVISFKGVKPEPSTIVKFF-NCCLITLAGLLKI 402
N L R C + +NM I++ KG S++V+ F C ++T G++ +
Sbjct: 167 NCLNRLCYGGSMEWNMVNVAILLMIKGKWINGSSVVRSFLPCAVVTSLGVVLV 219
>At1g78980.1 68414.m09209 leucine-rich repeat transmembrane protein
kinase, putative similar to leucine-rich repeat
transmembrane protein kinase 2 GI:3360291 from [Zea
mays]
Length = 693
Score = 26.2 bits (55), Expect = 8.5
Identities = 18/58 (31%), Positives = 27/58 (46%)
Frame = -3
Query: 338 SGFTPLKDITIGGIVMLNHTGEGEQVLVSRLPHSARRQRKRRSLNLQSLLNIWMIDVL 165
S +TP D+ G+VML ++L R+P + R RSL + + ID L
Sbjct: 571 SAYTPKSDVYSFGVVML-------ELLTGRVPFDGSKPRPERSLVRWATPQLHDIDAL 621
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,624,039
Number of Sequences: 28952
Number of extensions: 134907
Number of successful extensions: 414
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 412
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 414
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 605614832
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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