BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0992
(656 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|... 38 0.001
SPAC31G5.11 |pac2||cAMP-independent regulatory protein Pac2 |Sch... 29 0.45
SPCC1442.09 |trp3||anthranilate synthase component I|Schizosacch... 26 4.2
SPBC2F12.14c |gua1||IMP dehydrogenase Gua1 |Schizosaccharomyces ... 26 4.2
SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom... 25 7.3
SPBC4.06 |||acid phosphatase |Schizosaccharomyces pombe|chr 2|||... 25 9.6
SPAC19G12.14 |its3||1-phosphatidylinositol-4-phosphate 5-kinase ... 25 9.6
>SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 532
Score = 38.3 bits (85), Expect = 0.001
Identities = 23/75 (30%), Positives = 36/75 (48%)
Frame = +2
Query: 257 MFLGDGMSVATLTAARTLLGQRRGQTGEESRLSFEHFPTVGLSKTYCLDAQVADSACSAS 436
M + DGM +L+ R+ + + G L EH +G S+T + + DSA A+
Sbjct: 64 MMVSDGMGPGSLSMTRSFVETLNDKEGYRLPLD-EHL--IGSSRTRSSSSLITDSAAGAT 120
Query: 437 AYLCGAKANLGTIGV 481
A+ C K G +GV
Sbjct: 121 AFSCANKTYNGAVGV 135
Score = 31.9 bits (69), Expect = 0.084
Identities = 17/36 (47%), Positives = 22/36 (61%)
Frame = +1
Query: 541 SIASWALDADRDAGIVTTTRVTHASPAGAYAHTADR 648
+I A +A GIV T+RVT A+PA AH A+R
Sbjct: 144 TILEAAKEAGYLTGIVVTSRVTDATPASFSAHAANR 179
>SPAC31G5.11 |pac2||cAMP-independent regulatory protein Pac2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 235
Score = 29.5 bits (63), Expect = 0.45
Identities = 19/51 (37%), Positives = 21/51 (41%)
Frame = +3
Query: 195 QSELGERAWYDGSSGYARNVSCSSETACPWPHSRPPARCSASAGDRLERSP 347
+ E G R W DG S A VS S T R P S L+RSP
Sbjct: 51 EREAGMRRWTDGKSWSASRVSGSFLTYREMEGKRKPYHHGLSTDGSLKRSP 101
>SPCC1442.09 |trp3||anthranilate synthase component
I|Schizosaccharomyces pombe|chr 3|||Manual
Length = 489
Score = 26.2 bits (55), Expect = 4.2
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +3
Query: 126 RRSLQTPSTPAPELESEYWSRDAQSELGERAWYDGSSGYARNV 254
++ L++P P PE + + +A+S +GE DG + N+
Sbjct: 184 KKKLESPEIPLPEQKKVHLGYEAKSNVGE----DGYKAFVSNL 222
>SPBC2F12.14c |gua1||IMP dehydrogenase Gua1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 524
Score = 26.2 bits (55), Expect = 4.2
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = -1
Query: 620 AGEACVTRVVVTIPASLSASRAQEAMEASWCAASVAADSGV 498
+G AC+T+ V+ + + AQ A AS V AD G+
Sbjct: 333 SGSACITQEVMACGRPQATAIAQVAEFASQFGIGVIADGGI 373
>SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 204
Score = 25.4 bits (53), Expect = 7.3
Identities = 15/41 (36%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -3
Query: 210 PPAHSGRRATSTPTPARGPALRASAGSASTPRHPG-GNRPY 91
PP++S A +TP + A A A +AS R G +PY
Sbjct: 63 PPSYSNSAAPATPAASASSAAPAPAPAASQNRAYGAAPQPY 103
>SPBC4.06 |||acid phosphatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 462
Score = 25.0 bits (52), Expect = 9.6
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +3
Query: 159 PELESEYWSRDAQSELGERAWYDG 230
P L E W RDA++ + E W+ G
Sbjct: 262 PNLRDEQWLRDAETAVVEE-WFGG 284
>SPAC19G12.14 |its3||1-phosphatidylinositol-4-phosphate 5-kinase
Its3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 742
Score = 25.0 bits (52), Expect = 9.6
Identities = 11/41 (26%), Positives = 21/41 (51%)
Frame = -3
Query: 210 PPAHSGRRATSTPTPARGPALRASAGSASTPRHPGGNRPYL 88
P + + S+P P+R P R+S+ ++ + P +R L
Sbjct: 137 PQLQNLKHQLSSPQPSRAPIDRSSSNPVTSSQQPPNDRSTL 177
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,263,412
Number of Sequences: 5004
Number of extensions: 40819
Number of successful extensions: 135
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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