Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0971
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase Dld1|Schi... 111 8e-26
SPBC17A3.07 |pgr1||glutathione reductase|Schizosaccharomyces pom... 38 0.001
SPBC3F6.03 |trr1|caf4|thioredoxin reductase Trr1|Schizosaccharom... 31 0.19
SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyc... 30 0.25
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 29 0.44
SPAC20G8.04c |||mitochondrial electron transfer flavoprotein-ubi... 28 1.0
SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modifica... 27 2.4
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 27 2.4
SPBC26H8.01 |thi2|nmt2|thiazole biosynthetic enzyme|Schizosaccha... 27 3.1
SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase Gut2|Schi... 26 4.1
SPBC776.03 |||homoserine dehydrogenase |Schizosaccharomyces pomb... 26 4.1
SPBC29A3.05 |||chromatin remodeling complex subunit|Schizosaccha... 25 9.5
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 25 9.5
>SPAC1002.09c |dld1|dldh|dihydrolipoamide dehydrogenase
Dld1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 511
Score = 111 bits (267), Expect = 8e-26
Identities = 50/85 (58%), Positives = 63/85 (74%)
Frame = +1
Query: 253 KDPTLGGTCLNVGCIPSKALLHNSHLYHMAKHDFKQRGIETGEVTFDFKKMMEYKANAVK 432
K TLGGTCLNVGCIPSKALL+NSH+YH KHD K+RGI+ V+ + +MM+ K ++VK
Sbjct: 76 KRGTLGGTCLNVGCIPSKALLNNSHIYHTVKHDTKRRGIDVSGVSVNLSQMMKAKDDSVK 135
Query: 433 GLTGGIAMLFQKNKVNLVKGVGTIV 507
LT GI LF+KNKV KG G+ +
Sbjct: 136 SLTSGIEYLFKKNKVEYAKGTGSFI 160
Score = 59.7 bits (138), Expect = 4e-10
Identities = 28/50 (56%), Positives = 36/50 (72%), Gaps = 2/50 (4%)
Frame = +3
Query: 510 PNKVEVHGEKGV--ETVNTKNILIASGSEVTPFPGVTFDEKQIITSTGAL 653
P + V G G +T+ KN +IA+GSEV PFPGVT DEK+I++STGAL
Sbjct: 162 PQTLSVKGIDGAADQTIKAKNFIIATGSEVKPFPGVTIDEKKIVSSTGAL 211
Score = 50.8 bits (116), Expect = 2e-07
Identities = 29/81 (35%), Positives = 47/81 (58%), Gaps = 1/81 (1%)
Frame = +2
Query: 128 RIATRQYATTHDADLVVIGSGPGGYVAAIKAAQLGMKVVSVE-RTLL*EVLVSMLDVYHQ 304
R+ + + + + DL VIG GPGGYVAAI+ AQLG+K + VE R L +++ + +
Sbjct: 34 RLYSAKASGNGEYDLCVIGGGPGGYVAAIRGAQLGLKTICVEKRGTLGGTCLNVGCIPSK 93
Query: 305 KLYCTTHIFTIWPNMTSSKGV 367
L +HI+ + T +G+
Sbjct: 94 ALLNNSHIYHTVKHDTKRRGI 114
>SPBC17A3.07 |pgr1||glutathione reductase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 464
Score = 37.9 bits (84), Expect = 0.001
Identities = 23/63 (36%), Positives = 35/63 (55%), Gaps = 1/63 (1%)
Frame = +1
Query: 265 LGGTCLNVGCIPSKALLHNSHLYHMAKHDFKQRGIETGEV-TFDFKKMMEYKANAVKGLT 441
LGGTC+N GC+P K + + + L K KQ G ++ +FD+ M++ K +A G
Sbjct: 42 LGGTCVNYGCVPKKIMWNIADLVAKMK-TAKQNGFPNSQLGSFDW-GMIKRKRDAYIGRL 99
Query: 442 GGI 450
GI
Sbjct: 100 NGI 102
>SPBC3F6.03 |trr1|caf4|thioredoxin reductase
Trr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 322
Score = 30.7 bits (66), Expect = 0.19
Identities = 17/36 (47%), Positives = 23/36 (63%)
Frame = +2
Query: 155 THDADLVVIGSGPGGYVAAIKAAQLGMKVVSVERTL 262
TH+ +V+IGSGP G+ AAI A+ +K V E L
Sbjct: 2 THNK-VVIIGSGPAGHTAAIYLARGELKPVMYEGML 36
>SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 457
Score = 30.3 bits (65), Expect = 0.25
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +2
Query: 155 THDADLVVIGSGPGGYVAAIKAAQLGMKVVSVERTL 262
T DAD+++IG+G G + G KV+ +ER +
Sbjct: 3 TQDADIIIIGAGITGCALGAALGRQGRKVLVLERDM 38
>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 536
Score = 29.5 bits (63), Expect = 0.44
Identities = 16/48 (33%), Positives = 29/48 (60%)
Frame = -3
Query: 297 YTSNIETSTS*SRVLSTETTFMPSWAALIAATYPPGPEPITTRSASCV 154
++S++ +S+S S T+ + S A+++++TY GP IT S S V
Sbjct: 146 HSSSVPSSSSSYHSSSMTTSGLSSSASIVSSTYRDGPSIITLVSTSYV 193
>SPAC20G8.04c |||mitochondrial electron transfer
flavoprotein-ubiquinone
oxidoreductase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 632
Score = 28.3 bits (60), Expect = 1.0
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +2
Query: 161 DADLVVIGSGPGGYVAAIKAAQLGMK 238
D D+ ++G+GP G AAI+ Q K
Sbjct: 90 DVDVCIVGAGPAGLSAAIRIKQQAAK 115
>SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl
modification enzyme|Schizosaccharomyces pombe|chr
2|||Manual
Length = 666
Score = 27.1 bits (57), Expect = 2.4
Identities = 15/48 (31%), Positives = 26/48 (54%), Gaps = 2/48 (4%)
Frame = +2
Query: 122 LVRIATRQYATTHDA--DLVVIGSGPGGYVAAIKAAQLGMKVVSVERT 259
+ R R + T+ + ++VVIG G G AA A++LG K + ++
Sbjct: 1 MFRTTKRYFCTSFNRRKNVVVIGGGHAGVEAAAAASRLGAKTTLLTKS 48
>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2111
Score = 27.1 bits (57), Expect = 2.4
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +2
Query: 170 LVVIGSGPGGYVAAIKAAQLGMKVVSVER 256
+ +IGSGP G AA + + G VV ER
Sbjct: 1758 VAIIGSGPAGLAAADQLNRAGHHVVIYER 1786
>SPBC26H8.01 |thi2|nmt2|thiazole biosynthetic
enzyme|Schizosaccharomyces pombe|chr 2|||Manual
Length = 328
Score = 26.6 bits (56), Expect = 3.1
Identities = 16/57 (28%), Positives = 31/57 (54%), Gaps = 6/57 (10%)
Frame = +2
Query: 110 RSGSLVRIATRQYATTHD----ADLVVIGSGPGGYVAA--IKAAQLGMKVVSVERTL 262
R ++ R TR+Y + D +D+V++G+G G AA I + +K+ +E ++
Sbjct: 55 RESTVSRAMTRRYFSDLDKYAESDIVIVGAGSAGLTAAYYIGTRRPDLKIAIIEASV 111
>SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase
Gut2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 649
Score = 26.2 bits (55), Expect = 4.1
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = +2
Query: 167 DLVVIGSGPGGYVAAIKAAQLGMKVVSVERT 259
D+++IG G G A+ A+ G+ V +E+T
Sbjct: 69 DVLIIGGGATGTGVAVDASTRGLNVCLLEKT 99
>SPBC776.03 |||homoserine dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 376
Score = 26.2 bits (55), Expect = 4.1
Identities = 16/48 (33%), Positives = 25/48 (52%)
Frame = +2
Query: 113 SGSLVRIATRQYATTHDADLVVIGSGPGGYVAAIKAAQLGMKVVSVER 256
S +++ T++Y H LVVIG+G G V A +K++S R
Sbjct: 325 SDNIISFTTKRY---HTRPLVVIGAGAGAAVTAAGVLGDMIKIMSQVR 369
>SPBC29A3.05 |||chromatin remodeling complex
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 139
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/36 (33%), Positives = 16/36 (44%), Gaps = 1/36 (2%)
Frame = +3
Query: 432 RPHRRYCNALSEEQG*SCQRCRNYSCPNKVEV-HGE 536
+P R++CN +CQ C C EV H E
Sbjct: 96 KPPRKFCNVCGYWGKYACQNCGTSYCSKGCEVIHSE 131
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = -3
Query: 465 LKEHCNTSCEAFNSISFIFHHLFEVKCNFTSFNTPLLE 352
+K+ + ++ SIS ++H L + N S N LLE
Sbjct: 778 MKQSFTSLVNSYQSISNLYHELRDDHVNMQSQNNTLLE 815
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,763,333
Number of Sequences: 5004
Number of extensions: 57785
Number of successful extensions: 189
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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