BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0968 (693 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 150 2e-37 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 147 1e-36 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 81 2e-16 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 55 1e-08 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.21 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.5 SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 27 3.4 SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces pombe... 26 4.5 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 4.5 SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom... 26 4.5 SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.5 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 4.5 SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.9 SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 26 5.9 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 7.9 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 150 bits (364), Expect = 2e-37 Identities = 69/85 (81%), Positives = 76/85 (89%), Gaps = 1/85 (1%) Frame = +1 Query: 259 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEIT 438 Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE SV EIT Sbjct: 233 QVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEIT 292 Query: 439 NACFEPANQMVKCDPRHGKYMA-CL 510 N CFEP NQMVKCDPR G+YMA CL Sbjct: 293 NQCFEPYNQMVKCDPRAGRYMATCL 317 Score = 141 bits (341), Expect = 1e-34 Identities = 61/82 (74%), Positives = 75/82 (91%) Frame = +2 Query: 2 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 181 SGF +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDN Sbjct: 147 SGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDN 206 Query: 182 EAIYDICRRNLDIERPTYTNLN 247 E+ YDICRRNLDIERP+Y NLN Sbjct: 207 ESCYDICRRNLDIERPSYENLN 228 Score = 100 bits (240), Expect = 2e-22 Identities = 42/61 (68%), Positives = 51/61 (83%) Frame = +3 Query: 510 MLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVC 689 +LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++AKV RAVC Sbjct: 317 LLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVC 376 Query: 690 M 692 M Sbjct: 377 M 377 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 147 bits (357), Expect = 1e-36 Identities = 68/85 (80%), Positives = 75/85 (88%), Gaps = 1/85 (1%) Frame = +1 Query: 259 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEIT 438 Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE SV EIT Sbjct: 237 QVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEIT 296 Query: 439 NACFEPANQMVKCDPRHGKYMA-CL 510 N CFEP NQMVKCDPR G+YMA CL Sbjct: 297 NQCFEPYNQMVKCDPRTGRYMATCL 321 Score = 145 bits (352), Expect = 5e-36 Identities = 63/82 (76%), Positives = 75/82 (91%) Frame = +2 Query: 2 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 181 SG +LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDN Sbjct: 151 SGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDN 210 Query: 182 EAIYDICRRNLDIERPTYTNLN 247 EA YDICRRNLDIERPTY NLN Sbjct: 211 EACYDICRRNLDIERPTYENLN 232 Score = 105 bits (251), Expect = 8e-24 Identities = 43/61 (70%), Positives = 53/61 (86%) Frame = +3 Query: 510 MLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVC 689 +LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV RAVC Sbjct: 321 LLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVC 380 Query: 690 M 692 M Sbjct: 381 M 381 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 80.6 bits (190), Expect = 2e-16 Identities = 33/80 (41%), Positives = 52/80 (65%) Frame = +1 Query: 262 IVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITN 441 +++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ + + + +SV E+T Sbjct: 232 VMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQ 291 Query: 442 ACFEPANQMVKCDPRHGKYM 501 F+ N MV DPRHG+Y+ Sbjct: 292 QMFDANNMMVAADPRHGRYL 311 Score = 72.5 bits (170), Expect = 5e-14 Identities = 33/82 (40%), Positives = 51/82 (62%) Frame = +2 Query: 2 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 181 SG +LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DN Sbjct: 145 SGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDN 204 Query: 182 EAIYDICRRNLDIERPTYTNLN 247 EA+ I L I+ P+Y +LN Sbjct: 205 EALSSIFANTLKIKSPSYDDLN 226 Score = 35.1 bits (77), Expect = 0.010 Identities = 15/43 (34%), Positives = 24/43 (55%) Frame = +3 Query: 513 LYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 641 L+RG V K+V+ I +++TK + FV+W P + PP Sbjct: 316 LFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPP 358 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 54.8 bits (126), Expect = 1e-08 Identities = 30/83 (36%), Positives = 45/83 (54%), Gaps = 1/83 (1%) Frame = +2 Query: 2 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVD 178 SG S L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D ++D Sbjct: 147 SGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLD 206 Query: 179 NEAIYDICRRNLDIERPTYTNLN 247 N A+ I L + PT+ N Sbjct: 207 NAALAHIAADRLHTQNPTFHQQN 229 Score = 54.0 bits (124), Expect = 2e-08 Identities = 28/89 (31%), Positives = 43/89 (48%), Gaps = 3/89 (3%) Frame = +1 Query: 226 PNLHQPESSHGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---A 396 P HQ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P + Sbjct: 223 PTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVE 282 Query: 397 EKAYHEQLSVAEITNACFEPANQMVKCDP 483 E + +V ++ P NQMV +P Sbjct: 283 EAKAIRKTTVLDVMRRLLLPKNQMVSVNP 311 Score = 33.5 bits (73), Expect = 0.030 Identities = 12/48 (25%), Positives = 27/48 (56%) Frame = +3 Query: 504 LLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTV 647 +L + +G+ P DV+ ++ I+ +R F+ W P +V ++ + P + Sbjct: 320 ILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYI 367 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 30.7 bits (66), Expect = 0.21 Identities = 16/45 (35%), Positives = 21/45 (46%) Frame = +1 Query: 520 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 654 VV P + RP++P P LS V PV+ V + PP P Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.9 bits (59), Expect = 1.5 Identities = 17/49 (34%), Positives = 26/49 (53%) Frame = +2 Query: 320 PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 466 P+++ P S + T +SS+P P N+ PSP S + AS+ P R Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279 >SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 793 Score = 26.6 bits (56), Expect = 3.4 Identities = 18/64 (28%), Positives = 24/64 (37%) Frame = +3 Query: 489 WQVHGLLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLA 668 W+ Y + KDVN + TI +W PTG ++ Y V D Sbjct: 141 WRHSSFAQYYLYNTETKDVNM-LGQDNEHWTISLAEWSPTGHQLSFVYNNDLYVRKND-G 198 Query: 669 KVQR 680 VQR Sbjct: 199 NVQR 202 >SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 455 Score = 26.2 bits (55), Expect = 4.5 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = +3 Query: 186 PSMTSAAVISTLSAQPTPT*IVSRTDCLL 272 P T A V TL +P I++R+DC L Sbjct: 33 PKRTYALVCETLKYKPVLDEIIARSDCFL 61 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +2 Query: 383 QSSLPRRPTMNSFPSPRSQTHASSPPTR 466 + SLPRRP+ +P S T ++ PP + Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765 >SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 429 Score = 26.2 bits (55), Expect = 4.5 Identities = 7/17 (41%), Positives = 13/17 (76%) Frame = -1 Query: 687 RRHVEPWPSRLRAPRWV 637 +R+ + WPS++ P+WV Sbjct: 153 QRYSDSWPSKITLPKWV 169 >SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual Length = 1339 Score = 26.2 bits (55), Expect = 4.5 Identities = 14/47 (29%), Positives = 27/47 (57%) Frame = +3 Query: 495 VHGLLMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQ 635 V G + L R ++ + N AIA+I +I+F ++ P GF ++++ Sbjct: 294 VRGAVRLMRFLMLQEVKNDAIASITLSSSIEFTEF-PLGFNENVDFE 339 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 26.2 bits (55), Expect = 4.5 Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%) Frame = +1 Query: 556 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 642 +LP KPS+ +++S V+P S STTS+P Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36 >SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 751 Score = 25.8 bits (54), Expect = 5.9 Identities = 16/47 (34%), Positives = 26/47 (55%) Frame = +2 Query: 317 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 457 SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664 >SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 25.8 bits (54), Expect = 5.9 Identities = 16/51 (31%), Positives = 31/51 (60%) Frame = +2 Query: 344 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 496 LTP + S +S + +S +R ++N+ PS + +H+SS T + PV+++ Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = +1 Query: 553 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 648 P PS+P+++SN ST G+Q V V + W Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,012,471 Number of Sequences: 5004 Number of extensions: 64006 Number of successful extensions: 238 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 214 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 237 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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