BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0949
(668 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1||... 27 1.8
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 27 3.2
SPAC23C11.11 |cka1|orb5|serine/threonine protein kinase Cka1|Sch... 26 5.6
SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos... 26 5.6
SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces po... 26 5.6
SPAC8E11.05c |||conserved fungal protein|Schizosaccharomyces pom... 25 9.9
>SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 753
Score = 27.5 bits (58), Expect = 1.8
Identities = 18/55 (32%), Positives = 25/55 (45%)
Frame = +2
Query: 20 LLCSVAYGSNLTFPPGFKFGAASASYQVEGAWNVSDKGESIWDRLVHTKPEAIMD 184
LL + G N P K G+ SAS++ + AW++ K S R T E D
Sbjct: 496 LLQELISGINQKQKPSHKRGSLSASHKRDAAWSLLYKATSNKSRPTTTSTELKTD 550
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 26.6 bits (56), Expect = 3.2
Identities = 12/48 (25%), Positives = 27/48 (56%), Gaps = 3/48 (6%)
Frame = +1
Query: 343 YYNNLIDGLLDKGIEPLVTIFHWD---LPQSLQDLGGWMNPLIVDWFE 477
YY+ DG +D I+P + F +D + +++++L ++ + WF+
Sbjct: 4665 YYDTKEDGTMDLKIQPYIDEFAFDYYLVVRNIEELPQLLSSALRQWFQ 4712
>SPAC23C11.11 |cka1|orb5|serine/threonine protein kinase
Cka1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 332
Score = 25.8 bits (54), Expect = 5.6
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = -3
Query: 480 VLKPVDYQRIHPATKILQTL 421
VLKPV Y++I KILQ L
Sbjct: 73 VLKPVKYKKIKREIKILQNL 92
>SPBC13G1.03c |pex14||peroxisomal membrane anchor
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 25.8 bits (54), Expect = 5.6
Identities = 12/36 (33%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
Frame = -3
Query: 189 VRSIIASGLVWTSRSHMLSPLSLTFQAPS-TWYEAD 85
+ +I++G W++ S + ++ F+APS YEAD
Sbjct: 74 IMGVISTGFAWSAYSLVKKYIAPMFRAPSQNAYEAD 109
>SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 507
Score = 25.8 bits (54), Expect = 5.6
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = +2
Query: 29 SVAYGSNLTFPPGFKFGAASASYQVEGAWNVSDKGESIW 145
S +G++L+F AS+S +E SDK SIW
Sbjct: 298 SSCFGNSLSFAASNGVDGASSSQVLENILVASDKEFSIW 336
>SPAC8E11.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 338
Score = 25.0 bits (52), Expect = 9.9
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +1
Query: 466 DWFEDYARVVFSLFGIELKPGSL*TSCIDLRLCL*HWR 579
DW +D+ V S+FG+ P ++ S +D L W+
Sbjct: 144 DWQDDFQDAVNSVFGLPETPKTIEESSLDPDRILSLWQ 181
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,930,377
Number of Sequences: 5004
Number of extensions: 61587
Number of successful extensions: 147
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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