Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0931
(400 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family pr... 37 0.006
At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolas... 31 0.28
At3g60510.1 68416.m06768 enoyl-CoA hydratase/isomerase family pr... 30 0.49
At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolas... 28 2.6
At2g38185.4 68415.m04692 zinc finger (C3HC4-type RING finger) fa... 27 3.5
At2g38185.3 68415.m04691 zinc finger (C3HC4-type RING finger) fa... 27 3.5
At2g38185.2 68415.m04690 zinc finger (C3HC4-type RING finger) fa... 27 3.5
At2g38185.1 68415.m04689 zinc finger (C3HC4-type RING finger) fa... 27 3.5
At1g06550.1 68414.m00694 enoyl-CoA hydratase/isomerase family pr... 27 3.5
At5g56110.1 68418.m07000 myb family transcription factor contain... 27 4.6
At1g29940.1 68414.m03658 DNA-directed RNA polymerase family prot... 27 4.6
At5g05970.1 68418.m00661 transducin family protein / WD-40 repea... 27 6.1
At3g42460.1 68416.m04398 hypothetical protein several hypothetic... 27 6.1
At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolas... 27 6.1
At5g42120.1 68418.m05128 lectin protein kinase family protein co... 26 8.0
At5g33280.1 68418.m03944 chloride channel-like (CLC) protein, pu... 26 8.0
At4g31810.1 68417.m04521 enoyl-CoA hydratase/isomerase family pr... 26 8.0
At3g24360.1 68416.m03058 enoyl-CoA hydratase/isomerase family pr... 26 8.0
>At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family
protein similar to 3-hydroxybutyryl-CoA dehydratase
(Crotonase) from Clostridium acetobutylicum [SP|P52046],
FadB1x (enoyl-CoA hydratase) from Pseudomonas putida
[GI:13310130]; contains Pfam profile PF00378 enoyl-CoA
hydratase/isomerase family protein
Length = 265
Score = 36.7 bits (81), Expect = 0.006
Identities = 13/56 (23%), Positives = 30/56 (53%)
Frame = +3
Query: 195 SVKPPKGTECSMQTIVCRTRKAVNDFDADSNIAAIIITGNEKAFAAGADIKEMQTI 362
++ PK + ++ KA D D+D ++ +I TG+ ++F +G D+ +++
Sbjct: 23 TINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGSGRSFCSGVDLTAAESV 78
Score = 31.1 bits (67), Expect = 0.28
Identities = 13/38 (34%), Positives = 25/38 (65%)
Frame = +1
Query: 145 ENIKVEVVGSKKNVGLIQLNRPKALNALCKPLFVELGR 258
EN+ ++V + +I +NRPK+LN+L + + V+L +
Sbjct: 7 ENL-IQVKKESGGIAVITINRPKSLNSLTRAMMVDLAK 43
>At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 378
Score = 31.1 bits (67), Expect = 0.28
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +1
Query: 163 VVGSKKNVGLIQLNRPKALNALC 231
+V K +V ++ LNRPK LNALC
Sbjct: 8 LVEEKSSVRILTLNRPKQLNALC 30
Score = 28.3 bits (60), Expect = 2.0
Identities = 12/53 (22%), Positives = 25/53 (47%)
Frame = +3
Query: 186 RTHSVKPPKGTECSMQTIVCRTRKAVNDFDADSNIAAIIITGNEKAFAAGADI 344
R ++ PK ++ R + ++ D ++ +I+ G +AF AG D+
Sbjct: 16 RILTLNRPKQLNALCFNMISRLLQLFRAYEEDPSVKLVILKGQGRAFCAGGDV 68
>At3g60510.1 68416.m06768 enoyl-CoA hydratase/isomerase family
protein similar to enoyl-CoA-hydratase, Avicennia
marina, EMBL:AF190450 [GI:6014701], CoA-thioester
hydrolase CHY1 from Arabidopsis thaliana [GI:8572760];
contains Pfam profile PF00378 enoyl-CoA
hydratase/isomerase family protein
Length = 401
Score = 30.3 bits (65), Expect = 0.49
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +3
Query: 246 RTRKAVNDFDADSNIAAIIITGNEKAFAAGADI 344
R +K +++ D NI +++ G+ +AF AG DI
Sbjct: 68 RLQKLYKNWEEDPNIGFVMMKGSGRAFCAGGDI 100
>At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolase /
CoA-thioester hydrolase (CHY1) identical to gi:8572760;
contains Pfam profile PF00388 enoyl-CoA
hydratase/isomerase family protein
Length = 378
Score = 27.9 bits (59), Expect = 2.6
Identities = 13/53 (24%), Positives = 26/53 (49%)
Frame = +3
Query: 186 RTHSVKPPKGTECSMQTIVCRTRKAVNDFDADSNIAAIIITGNEKAFAAGADI 344
R ++ PK ++ R + F+ D ++ +I+ G+ +AF AG D+
Sbjct: 20 RILTLNRPKQLNALSFHMISRLLQLFLAFEEDPSVKLVILKGHGRAFCAGGDV 72
Score = 27.5 bits (58), Expect = 3.5
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +1
Query: 163 VVGSKKNVGLIQLNRPKALNAL 228
+V K +V ++ LNRPK LNAL
Sbjct: 12 LVEEKSSVRILTLNRPKQLNAL 33
>At2g38185.4 68415.m04692 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 872
Score = 27.5 bits (58), Expect = 3.5
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = -3
Query: 386 NLFVCC*MYGLHLLNISTSRKRLLVTSDDDGSNV 285
N F C G HL ++R RLL DDDGS++
Sbjct: 327 NRFQCSGGEG-HLTEDDSARTRLLADKDDDGSSM 359
>At2g38185.3 68415.m04691 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 346
Score = 27.5 bits (58), Expect = 3.5
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = -3
Query: 386 NLFVCC*MYGLHLLNISTSRKRLLVTSDDDGSNV 285
N F C G HL ++R RLL DDDGS++
Sbjct: 231 NRFQCSGGEG-HLTEDDSARTRLLADKDDDGSSM 263
>At2g38185.2 68415.m04690 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 441
Score = 27.5 bits (58), Expect = 3.5
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = -3
Query: 386 NLFVCC*MYGLHLLNISTSRKRLLVTSDDDGSNV 285
N F C G HL ++R RLL DDDGS++
Sbjct: 326 NRFQCSGGEG-HLTEDDSARTRLLADKDDDGSSM 358
>At2g38185.1 68415.m04689 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 441
Score = 27.5 bits (58), Expect = 3.5
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = -3
Query: 386 NLFVCC*MYGLHLLNISTSRKRLLVTSDDDGSNV 285
N F C G HL ++R RLL DDDGS++
Sbjct: 326 NRFQCSGGEG-HLTEDDSARTRLLADKDDDGSSM 358
>At1g06550.1 68414.m00694 enoyl-CoA hydratase/isomerase family
protein similar to CHY1 [gi:8572760]; contains Pfam
profile PF00388 enoyl-CoA hydratase/isomerase family
protein
Length = 387
Score = 27.5 bits (58), Expect = 3.5
Identities = 14/61 (22%), Positives = 28/61 (45%)
Frame = +3
Query: 165 GRLQEECRTHSVKPPKGTECSMQTIVCRTRKAVNDFDADSNIAAIIITGNEKAFAAGADI 344
G + R ++ P+ +V + + + ++ D I+I G +AF+AG D+
Sbjct: 15 GEEKGSVRLTTLNRPRQLNVISPEVVFKLAEYLELWEKDDQTKLILIKGTGRAFSAGGDL 74
Query: 345 K 347
K
Sbjct: 75 K 75
>At5g56110.1 68418.m07000 myb family transcription factor contains
PFAM profile: Myb DNA binding domain PF00249
Length = 320
Score = 27.1 bits (57), Expect = 4.6
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 59 CWERMY*TSAKWYPQQAKHP*SFIAQHHTRTSRL 160
C E+ +W P++ S+IAQH TR RL
Sbjct: 6 CCEKENVKRGQWTPEEDNKLASYIAQHGTRNWRL 39
>At1g29940.1 68414.m03658 DNA-directed RNA polymerase family protein
similar to SP|P22138 DNA-directed RNA polymerase I 135
kDa polypeptide (EC 2.7.7.6) (RNA polymerase I subunit
2) {Saccharomyces cerevisiae}; contains Pfam profiles
PF04563; RNA polymerase beta subunit, PF04560: RNA
polymerase Rpb2 domain 7, PF04561: RNA polymerase Rpb2
domain 2, PF04565: RNA polymerase Rpb2 domain 3,
PF00562: RNA polymerase Rpb2 domain 6
Length = 1114
Score = 27.1 bits (57), Expect = 4.6
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = +3
Query: 198 VKPPKGTECSMQTIVCRTRKAVNDFDADSNI 290
V P GT C + + RT + + FD+ NI
Sbjct: 444 VHTPDGTPCGLLNHMTRTSRITSQFDSKGNI 474
>At5g05970.1 68418.m00661 transducin family protein / WD-40 repeat
family protein contains similarity to regulatory protein
Nedd1; contains Pfam PF00400: WD domain, G-beta repeat
(6 copies, 2 weak)|19804256|gb|AV785466.1|AV785466
Length = 781
Score = 26.6 bits (56), Expect = 6.1
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = -3
Query: 356 LHLLNISTSRKRLLVTSDDDGS 291
L LL+ S S + LLVT+ DDG+
Sbjct: 181 LRLLDYSRSSRHLLVTAGDDGT 202
>At3g42460.1 68416.m04398 hypothetical protein several hypothetical
proteins - Arabidopsis thaliana
Length = 202
Score = 26.6 bits (56), Expect = 6.1
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = +1
Query: 49 TRALLGKNVLNKCKVVSATSQASIKFYSTASYENIKVEVVGSKKNVGLIQL 201
T A G N+++K V S IKF + S++NI + G+ +N L+ L
Sbjct: 61 TSAEYGINLMDKTVVTSGDPNTPIKFNNFTSFDNI---LEGTFENDQLVDL 108
>At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 422
Score = 26.6 bits (56), Expect = 6.1
Identities = 12/46 (26%), Positives = 23/46 (50%)
Frame = +3
Query: 207 PKGTECSMQTIVCRTRKAVNDFDADSNIAAIIITGNEKAFAAGADI 344
PK +V R + ++ D ++ +++ G +AF+AG DI
Sbjct: 67 PKQLNALSFHMVSRLLQLFLAYEEDPSVKLVVLKGQGRAFSAGGDI 112
>At5g42120.1 68418.m05128 lectin protein kinase family protein
contains Pfam domains PF00139: Legume lectins beta
domain and PF00069: Protein kinase domain
Length = 691
Score = 26.2 bits (55), Expect = 8.0
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = -3
Query: 260 CLPSSTNNGLHRAFSAFGRFN*MSPTFFLEPTTST 156
C PS T + S GR + P FLEP+T+T
Sbjct: 56 CFPSVTTPPSKPSSSGIGRALYVYPIKFLEPSTNT 90
>At5g33280.1 68418.m03944 chloride channel-like (CLC) protein,
putative similar to CLC-c, At5g49890 [Arabidopsis
thaliana] and chloride channel protein ClC-1 - Nicotiana
tabacum, PIR:T02939
Length = 763
Score = 26.2 bits (55), Expect = 8.0
Identities = 17/46 (36%), Positives = 20/46 (43%), Gaps = 1/46 (2%)
Frame = +1
Query: 10 WFIFLKMASVATVTRALLGKNVLNKCKVVSATSQASIKFYS-TASY 144
W IF A VA V RAL+ + KC + YS ASY
Sbjct: 263 WRIFFSTAVVAIVLRALIDVCLSGKCGLFGKGGLIMFDVYSENASY 308
>At4g31810.1 68417.m04521 enoyl-CoA hydratase/isomerase family
protein similar to CHY1 [gi:8572760]; contains Pfam
profile PF00388 enoyl-CoA hydratase/isomerase family
protein
Length = 409
Score = 26.2 bits (55), Expect = 8.0
Identities = 18/43 (41%), Positives = 24/43 (55%)
Frame = +1
Query: 130 STASYENIKVEVVGSKKNVGLIQLNRPKALNALCKPLFVELGR 258
S A +E+ +V V G K+ I LN P +LNAL P+ L R
Sbjct: 35 SDADFED-QVLVEGKAKSRAAI-LNNPSSLNALSAPMVGRLKR 75
>At3g24360.1 68416.m03058 enoyl-CoA hydratase/isomerase family
protein similar to CHY1 [gi:8572760]; contains Pfam
profile PF00388 enoyl-CoA hydratase/isomerase family
protein
Length = 418
Score = 26.2 bits (55), Expect = 8.0
Identities = 16/45 (35%), Positives = 25/45 (55%)
Frame = +1
Query: 94 VSATSQASIKFYSTASYENIKVEVVGSKKNVGLIQLNRPKALNAL 228
+S + + ++ S E +K V + V LI L+RPKALNA+
Sbjct: 28 LSVSHRRKFSVMASGSDEFVKGNVYPN--GVALITLDRPKALNAM 70
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,849,120
Number of Sequences: 28952
Number of extensions: 165585
Number of successful extensions: 442
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 433
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 442
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 575830496
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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