BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0919 (532 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g55700.1 68416.m06188 UDP-glucoronosyl/UDP-glucosyl transfera... 31 0.48 At5g31412.1 68418.m03722 hypothetical protein contains Pfam doma... 31 0.64 At2g45000.1 68415.m05603 expressed protein contains Pfam profile... 31 0.64 At4g21450.2 68417.m03102 vesicle-associated membrane family prot... 30 1.1 At4g21450.1 68417.m03103 vesicle-associated membrane family prot... 30 1.1 At4g02510.1 68417.m00343 chloroplast outer membrane protein, put... 29 2.6 At3g16800.2 68416.m02145 protein phosphatase 2C, putative / PP2C... 27 6.0 At3g16800.1 68416.m02146 protein phosphatase 2C, putative / PP2C... 27 6.0 At2g36420.1 68415.m04471 expressed protein 27 6.0 At3g57660.1 68416.m06424 DNA-directed RNA polymerase family prot... 27 7.9 >At3g55700.1 68416.m06188 UDP-glucoronosyl/UDP-glucosyl transferase family protein glucuronosyl transferase homolog, Lycopersicon esculentum, PIR:S39507 ;contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl transferase Length = 460 Score = 31.1 bits (67), Expect = 0.48 Identities = 23/68 (33%), Positives = 36/68 (52%), Gaps = 1/68 (1%) Frame = +1 Query: 10 GSISWVEYPA-ELDEDGNGLVIVDLPIEAQPEDLEKAQLVDLPVENVAEPEDLSPVQVVN 186 G+ S+ + A L D L I D ++ +L ++ DLPV EPE+L +VVN Sbjct: 141 GASSFCAFAAFPLLRDKGYLPIQDSRLDEPVTELPPLKVKDLPVMETNEPEEL--YRVVN 198 Query: 187 PIVENSQS 210 +VE ++S Sbjct: 199 DMVEGAKS 206 >At5g31412.1 68418.m03722 hypothetical protein contains Pfam domain, PF04937: Protein of unknown function (DUF 659) Length = 433 Score = 30.7 bits (66), Expect = 0.64 Identities = 17/51 (33%), Positives = 26/51 (50%) Frame = +1 Query: 28 EYPAELDEDGNGLVIVDLPIEAQPEDLEKAQLVDLPVENVAEPEDLSPVQV 180 E P+E DE+ NG + P E EDLE + D+ ++ E ++ V V Sbjct: 375 EGPSERDEEPNGEPVYQTPNEKPREDLESHEEHDITIDIEFESDNEQVVNV 425 >At2g45000.1 68415.m05603 expressed protein contains Pfam profile: PF05064 Nsp1-like C-terminal region Length = 739 Score = 30.7 bits (66), Expect = 0.64 Identities = 23/55 (41%), Positives = 30/55 (54%), Gaps = 2/55 (3%) Frame = -3 Query: 161 SSGSATFSTGRSTSW--AFSKSSGCASIGRSTITSPFPSSSNSAGYSTQLIEPTS 3 SS S +TG S S+ A S S SI +T +SPF SS+SAG + L +S Sbjct: 243 SSSSIFGATGSSPSFSVASSASGSSPSIFGATGSSPFFGSSSSAGSTPSLFASSS 297 >At4g21450.2 68417.m03102 vesicle-associated membrane family protein / VAMP family protein similar to VAP27 GI:6688926 [Nicotiana plumbaginifolia] Length = 212 Score = 29.9 bits (64), Expect = 1.1 Identities = 14/32 (43%), Positives = 19/32 (59%) Frame = -3 Query: 113 FSKSSGCASIGRSTITSPFPSSSNSAGYSTQL 18 F SSG + S TSPFPS ++S+ S+ L Sbjct: 23 FRNSSGHRNAASSAATSPFPSGASSSSTSSHL 54 >At4g21450.1 68417.m03103 vesicle-associated membrane family protein / VAMP family protein similar to VAP27 GI:6688926 [Nicotiana plumbaginifolia] Length = 295 Score = 29.9 bits (64), Expect = 1.1 Identities = 14/32 (43%), Positives = 19/32 (59%) Frame = -3 Query: 113 FSKSSGCASIGRSTITSPFPSSSNSAGYSTQL 18 F SSG + S TSPFPS ++S+ S+ L Sbjct: 23 FRNSSGHRNAASSAATSPFPSGASSSSTSSHL 54 >At4g02510.1 68417.m00343 chloroplast outer membrane protein, putative similar to chloroplast protein import component Toc159 [Pisum sativum] GI:8489806, chloroplast outer envelope protein 86 [Pisum sativum] GI:599958, GTP-binding protein [Pisum sativum] GI:576509 Length = 1503 Score = 28.7 bits (61), Expect = 2.6 Identities = 14/44 (31%), Positives = 26/44 (59%) Frame = +1 Query: 37 AELDEDGNGLVIVDLPIEAQPEDLEKAQLVDLPVENVAEPEDLS 168 A+++ G+V+V EA ++ +K VD + N+ EP+DL+ Sbjct: 477 ADINVAEPGVVVVGAAKEAVIKEDDKDDEVDKTISNIEEPDDLT 520 >At3g16800.2 68416.m02145 protein phosphatase 2C, putative / PP2C, putative similar to protein phosphatase-2C GB:AAC36699 from [Mesembryanthemum crystallinum] Length = 351 Score = 27.5 bits (58), Expect = 6.0 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +1 Query: 37 AELDEDGNGLVIVDLPIEAQPEDLEKAQLV 126 A +DGNGLV V L ++ +P E+A+ + Sbjct: 196 ATTSDDGNGLVPVQLSVDFKPNIPEEAERI 225 >At3g16800.1 68416.m02146 protein phosphatase 2C, putative / PP2C, putative similar to protein phosphatase-2C GB:AAC36699 from [Mesembryanthemum crystallinum] Length = 351 Score = 27.5 bits (58), Expect = 6.0 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +1 Query: 37 AELDEDGNGLVIVDLPIEAQPEDLEKAQLV 126 A +DGNGLV V L ++ +P E+A+ + Sbjct: 196 ATTSDDGNGLVPVQLSVDFKPNIPEEAERI 225 >At2g36420.1 68415.m04471 expressed protein Length = 439 Score = 27.5 bits (58), Expect = 6.0 Identities = 14/48 (29%), Positives = 24/48 (50%) Frame = +1 Query: 94 QPEDLEKAQLVDLPVENVAEPEDLSPVQVVNPIVENSQSEYPGKRYPD 237 + E LEK + + + + E SPV V++P+ E + E + PD Sbjct: 227 ETESLEKVRGQEEEDKEEEDKEQCSPVSVLDPLEEEEEDEDHHQHEPD 274 >At3g57660.1 68416.m06424 DNA-directed RNA polymerase family protein similar to SP|O35134 DNA-directed RNA polymerase I largest subunit (EC 2.7.7.6) (RNA polymerase I 194 kDa subunit) (RPA194) {Mus musculus}; contains InterPro accession IPR000722: RNA polymerase, alpha subunit Length = 1670 Score = 27.1 bits (57), Expect = 7.9 Identities = 19/78 (24%), Positives = 35/78 (44%), Gaps = 3/78 (3%) Frame = +1 Query: 13 SISWVEYPAELDEDGNGLVIVDLPIEAQPEDLEKAQLVDL---PVENVAEPEDLSPVQVV 183 S+S + + D+DG G + DL +AQ + ++ +D + EP +S V+ Sbjct: 1331 SVSGKQNEDDGDDDGEGTEVDDLGSDAQKQKKQETDEMDYEENSEDETNEPSSISGVEDP 1390 Query: 184 NPIVENSQSEYPGKRYPD 237 EN +E + P+ Sbjct: 1391 EMDSENEDTEVSKEDTPE 1408 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,071,294 Number of Sequences: 28952 Number of extensions: 185637 Number of successful extensions: 564 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 551 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 564 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 987020800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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