BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0913 (555 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma... 27 2.5 SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 26 3.2 SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz... 26 4.3 SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 25 5.7 SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosa... 25 5.7 SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces ... 25 7.5 SPBC16H5.15 |||conserved fungal protein|Schizosaccharomyces pomb... 25 7.5 SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 25 9.9 SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7 |Schizos... 25 9.9 SPBC29A10.11c |vps902|vps9b|guanyl-nucleotide exchange factor Vp... 25 9.9 >SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Manual Length = 918 Score = 26.6 bits (56), Expect = 2.5 Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 3/50 (6%) Frame = -2 Query: 539 FIECNVLIMYFVVWVRFLVVYSFHLLLLKPF---SLFAFYLQVFLYLQFE 399 F + NV+ + V F ++YSF L +P SLF+ +LQ+ L +Q + Sbjct: 712 FAKINVIERSYFV---FALLYSFLSFLQRPLGHLSLFSCFLQILLLIQLK 758 >SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizosaccharomyces pombe|chr 1|||Manual Length = 1290 Score = 26.2 bits (55), Expect = 3.2 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = +3 Query: 150 TKPSLQASLTDNIENAINKTQEKDVAAQKELFILWKKQGS 269 TK + Q + + +E INK+ E +K+L + KQGS Sbjct: 866 TKLNFQVNELEQLEKDINKSSEDCDLQKKKLLEVSSKQGS 905 >SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schizosaccharomyces pombe|chr 2|||Manual Length = 689 Score = 25.8 bits (54), Expect = 4.3 Identities = 18/69 (26%), Positives = 33/69 (47%) Frame = +3 Query: 150 TKPSLQASLTDNIENAINKTQEKDVAAQKELFILWKKQGS*NLKSNHIVLTE*NVYRLLK 329 TK ASL +EN N + K++ ++E L +K + + N + L V + L+ Sbjct: 52 TKNPKLASLEFQLENLKNDLKRKELEFEREQIELQRKLAEEHEQKNSLQLRLTLVEKQLE 111 Query: 330 RNRDNYKKK 356 +Y+K+ Sbjct: 112 EQSTSYQKE 120 >SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 25.4 bits (53), Expect = 5.7 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = +2 Query: 407 EDKEKLVDKTQTKKKVLTAIDENYIPPEILP 499 E EKL+ +T + + A D I PE+LP Sbjct: 279 EKNEKLLQNVKTSEIPINAADLEGINPELLP 309 >SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 660 Score = 25.4 bits (53), Expect = 5.7 Identities = 13/47 (27%), Positives = 20/47 (42%) Frame = +2 Query: 380 NEEKIDLQIEDKEKLVDKTQTKKKVLTAIDENYIPPEILPKRRNTLL 520 N+E+I + K TKK++ D +P E +P N L Sbjct: 59 NDEQISELVNGVNSTTIKENTKKEISNPNDSTRLPLEQIPGSNNLFL 105 >SPBC16D10.08c |||heat shock protein Hsp104 |Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 25.0 bits (52), Expect = 7.5 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = +2 Query: 245 YSLEKTRQLKLEEQSHRLDRVK 310 Y LEK+R +L++ RLD +K Sbjct: 466 YELEKSRGSELQDAKRRLDELK 487 >SPBC16H5.15 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 25.0 bits (52), Expect = 7.5 Identities = 13/45 (28%), Positives = 25/45 (55%) Frame = +2 Query: 368 WFFENEEKIDLQIEDKEKLVDKTQTKKKVLTAIDENYIPPEILPK 502 WF E+E++ + ++KE+L+ + + ++K L PE PK Sbjct: 68 WFRESEKRELMWKKEKEQLLKEKELRQKALDKERMVSSKPETNPK 112 >SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr 3|||Manual Length = 923 Score = 24.6 bits (51), Expect = 9.9 Identities = 9/16 (56%), Positives = 10/16 (62%) Frame = -3 Query: 277 KFQLPCFFQRINNSFC 230 KF P +FQR SFC Sbjct: 906 KFTQPSYFQRYGGSFC 921 >SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 758 Score = 24.6 bits (51), Expect = 9.9 Identities = 13/32 (40%), Positives = 18/32 (56%), Gaps = 1/32 (3%) Frame = -2 Query: 449 FSLFA-FYLQVFLYLQFEGQFFPHSQRTTAFA 357 F+ F F+LQ+F + F FFPH +T A Sbjct: 3 FAFFGIFWLQIFGSILFLLGFFPHKNDSTGKA 34 >SPBC29A10.11c |vps902|vps9b|guanyl-nucleotide exchange factor Vps902 |Schizosaccharomyces pombe|chr 2|||Manual Length = 402 Score = 24.6 bits (51), Expect = 9.9 Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 4/42 (9%) Frame = +2 Query: 371 FFENEEKIDL----QIEDKEKLVDKTQTKKKVLTAIDENYIP 484 F +EEK +L I D EKL ++ + A+DE + P Sbjct: 148 FIASEEKKELINCIPIPDDEKLTNRLHEVSEAFFALDEQHTP 189 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,788,523 Number of Sequences: 5004 Number of extensions: 30187 Number of successful extensions: 117 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 114 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 117 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 231978230 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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