BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0896
(503 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC29B5.03c |rpl26||60S ribosomal protein L26|Schizosaccharomyc... 111 4e-26
SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase... 27 2.1
SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-... 25 4.9
SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces pom... 25 6.5
>SPBC29B5.03c |rpl26||60S ribosomal protein L26|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 126
Score = 111 bits (268), Expect = 4e-26
Identities = 51/89 (57%), Positives = 72/89 (80%), Gaps = 1/89 (1%)
Frame = +2
Query: 20 MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 199
MKF++ VTSSRRK RK HF APS +RRVLMS+PLSKELR+++ ++S+P+R+DD++ V+RG
Sbjct: 1 MKFSRDVTSSRRKQRKAHFGAPSSVRRVLMSAPLSKELREQYKIRSLPVRRDDQITVIRG 60
Query: 200 HYKGQQVGKVMQVYRKKFVYTL-RGFKEK 283
KG++ GK+ VYRKKF+ + R +EK
Sbjct: 61 SNKGRE-GKITSVYRKKFLLLIERVTREK 88
Score = 53.2 bits (122), Expect = 2e-08
Identities = 26/46 (56%), Positives = 34/46 (73%)
Frame = +1
Query: 247 KVCVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRR 384
K + IER+ REKANGA+A VGI SK VI KL ++KDRK ++ R+
Sbjct: 76 KFLLLIERVTREKANGASAPVGIDASKVVITKLHLDKDRKDLIVRK 121
>SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4
desaturase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 362
Score = 26.6 bits (56), Expect = 2.1
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 8/63 (12%)
Frame = -2
Query: 328 TLKGECRHML----LLHWPFL----FESSQCIHKLFTIHLHHFANLLAFVVSTYNLNFIV 173
+L+ C ++L LL W F F + C LF + +H ++ L F + YN + +
Sbjct: 71 SLQFTCAYLLSQSSLLSWKFFLTAYFIGAFCNQNLF-LAIHELSHNLGFKKTLYNRAYCL 129
Query: 172 FAN 164
FAN
Sbjct: 130 FAN 132
>SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase
[UDP-forming]|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 25.4 bits (53), Expect = 4.9
Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = +1
Query: 307 VGIHPSKCV-IVKLKMNKDRKAILDRRAKGRLAALGKDKGKY 429
+GI P K +K + KDR A ++RR +G +G D+ Y
Sbjct: 256 IGIDPEKFSDALKSDVVKDRIASIERRLQGVKVIVGVDRLDY 297
>SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 986
Score = 25.0 bits (52), Expect = 6.5
Identities = 11/27 (40%), Positives = 13/27 (48%)
Frame = -3
Query: 429 VFTLVFAKCSQSALCSAIEDCFAVFIH 349
+F LV S +C IED F IH
Sbjct: 588 LFNLVATNASDPYICGIIEDTFEDIIH 614
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,071,356
Number of Sequences: 5004
Number of extensions: 44224
Number of successful extensions: 133
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 200198394
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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