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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0896
         (503 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g67510.1 68418.m08513 60S ribosomal protein L26 (RPL26B)           127   4e-30
At3g49910.1 68416.m05456 60S ribosomal protein L26 (RPL26A) 60S ...   122   1e-28
At1g28070.1 68414.m03436 expressed protein ; expression supporte...    28   3.1  
At4g35380.1 68417.m05026 guanine nucleotide exchange family prot...    27   5.4  
At4g02110.1 68417.m00282 BRCT domain-containing protein contains...    27   9.5  

>At5g67510.1 68418.m08513 60S ribosomal protein L26 (RPL26B)
          Length = 146

 Score =  127 bits (307), Expect = 4e-30
 Identities = 61/79 (77%), Positives = 72/79 (91%)
 Frame = +2

Query: 20  MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 199
           MKFN +V+SSRRKNRK HF+APS +RRVLMSSPLSK+LR K NV+SMPIRKDDEVQVVRG
Sbjct: 1   MKFNPRVSSSRRKNRKAHFTAPSSVRRVLMSSPLSKDLRNKHNVRSMPIRKDDEVQVVRG 60

Query: 200 HYKGQQVGKVMQVYRKKFV 256
            +KG++ GKVMQVYR+K+V
Sbjct: 61  TFKGRE-GKVMQVYRRKWV 78



 Score = 66.1 bits (154), Expect = 1e-11
 Identities = 31/59 (52%), Positives = 45/59 (76%)
 Frame = +1

Query: 247 KVCVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRRAKGRLAALGKDKG 423
           K  ++IERI REK NG+T  VG++ S  +I KL+++KDRK++L+R+A GR AA  K+KG
Sbjct: 76  KWVIHIERITREKVNGSTVNVGVNASNVMITKLRLDKDRKSLLERKANGR-AAADKEKG 133


>At3g49910.1 68416.m05456 60S ribosomal protein L26 (RPL26A) 60S
           RIBOSOMAL PROTEIN L26, Brassica rapa, EMBL:BRD495
          Length = 146

 Score =  122 bits (294), Expect = 1e-28
 Identities = 58/79 (73%), Positives = 71/79 (89%)
 Frame = +2

Query: 20  MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 199
           MK+N +VTSSRRKNRK HF+A S  RRV+MSSPLS +LRQK+NV+SMPIRKDDEVQ+VRG
Sbjct: 1   MKYNPRVTSSRRKNRKAHFTASSSERRVIMSSPLSTDLRQKYNVRSMPIRKDDEVQIVRG 60

Query: 200 HYKGQQVGKVMQVYRKKFV 256
            YKG++ GKV+QVYR+K+V
Sbjct: 61  TYKGRE-GKVVQVYRRKWV 78



 Score = 74.1 bits (174), Expect = 5e-14
 Identities = 36/59 (61%), Positives = 46/59 (77%)
 Frame = +1

Query: 247 KVCVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRRAKGRLAALGKDKG 423
           K  ++IERI REK NG T  VGI PSK VI KL+++KDRK++L+R+AKGR AA  K+KG
Sbjct: 76  KWVIHIERITREKVNGTTVNVGIQPSKVVITKLRLDKDRKSLLERKAKGR-AAADKEKG 133


>At1g28070.1 68414.m03436 expressed protein ; expression supported
           by MPSS
          Length = 176

 Score = 28.3 bits (60), Expect = 3.1
 Identities = 17/61 (27%), Positives = 28/61 (45%)
 Frame = +2

Query: 44  SSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRGHYKGQQVG 223
           S R      HFS PSH+ + L     + E+  +     M +++D    + RG+Y  Q  G
Sbjct: 38  SDRFLELSSHFSVPSHLEQCL--DLKTGEIYYRSWNSGMRVKEDPRKSMSRGNYADQSSG 95

Query: 224 K 226
           +
Sbjct: 96  E 96


>At4g35380.1 68417.m05026 guanine nucleotide exchange family protein
            similar to guanine nucleotide exchange factor [Homo
            sapiens] GI:5456754; contains Pfam profile PF01369: Sec7
            domain
          Length = 1706

 Score = 27.5 bits (58), Expect = 5.4
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 45   PQEGKTGRGISVLLHI 92
            P +G TG GISVLLH+
Sbjct: 1408 PFQGSTGSGISVLLHL 1423


>At4g02110.1 68417.m00282 BRCT domain-containing protein contains
           Pfam domain, PF00533: BRCA1 C Terminus (BRCT) domain
          Length = 1293

 Score = 26.6 bits (56), Expect = 9.5
 Identities = 13/53 (24%), Positives = 29/53 (54%)
 Frame = +2

Query: 23  KFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDE 181
           K     ++S  ++ +RH S  ++ R+ L  SP +  L ++ + ++  +R DD+
Sbjct: 269 KMETDGSTSINRSIRRHSSLATYSRKTLQRSPETDTLGKESSGQNRSLRMDDK 321


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,883,975
Number of Sequences: 28952
Number of extensions: 236801
Number of successful extensions: 639
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 627
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 637
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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