SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0887
         (581 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...   122   5e-29
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...   122   5e-29
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce...    97   1e-21
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma...    79   6e-16
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz...    41   2e-04
SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar...    27   2.0  
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    27   2.6  
SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy...    25   6.1  
SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31 |...    25   6.1  

>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score =  122 bits (293), Expect = 5e-29
 Identities = 56/88 (63%), Positives = 66/88 (75%)
 Frame = +2

Query: 2   GGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVA 181
           GGQIIPT RR +YA  L A P + EPV+L EIQ  E A+GGIY VLN++RGHVF E Q  
Sbjct: 702 GGQIIPTARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRV 761

Query: 182 GTPMFIVKAYLPVNESFGFTADLRSTPA 265
           GTP++ +KAYLPVNESFGFT +LR   A
Sbjct: 762 GTPLYNIKAYLPVNESFGFTGELRQATA 789



 Score = 62.1 bits (144), Expect = 6e-11
 Identities = 27/46 (58%), Positives = 31/46 (67%)
 Frame = +1

Query: 259 TGGQAFPQCVFDHWQVLPGDPCEPHSKPYNVVQVTRKRKRLKVGLP 396
           T GQAFPQ VFDHW  + GDP +P SKP  +V   RKRK LK  +P
Sbjct: 788 TAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLKENVP 833


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score =  122 bits (293), Expect = 5e-29
 Identities = 56/88 (63%), Positives = 66/88 (75%)
 Frame = +2

Query: 2   GGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVA 181
           GGQIIPT RR +YA  L A P + EPV+L EIQ  E A+GGIY VLN++RGHVF E Q  
Sbjct: 702 GGQIIPTARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRV 761

Query: 182 GTPMFIVKAYLPVNESFGFTADLRSTPA 265
           GTP++ +KAYLPVNESFGFT +LR   A
Sbjct: 762 GTPLYNIKAYLPVNESFGFTGELRQATA 789



 Score = 62.1 bits (144), Expect = 6e-11
 Identities = 27/46 (58%), Positives = 31/46 (67%)
 Frame = +1

Query: 259 TGGQAFPQCVFDHWQVLPGDPCEPHSKPYNVVQVTRKRKRLKVGLP 396
           T GQAFPQ VFDHW  + GDP +P SKP  +V   RKRK LK  +P
Sbjct: 788 TAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLKENVP 833


>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 983

 Score = 97.5 bits (232), Expect = 1e-21
 Identities = 44/84 (52%), Positives = 59/84 (70%)
 Frame = +2

Query: 2    GGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVA 181
            GGQIIPT RR  Y+  LTA PRLMEPVY+ E+  P  ++  IY +L RRRGHV ++    
Sbjct: 818  GGQIIPTARRVCYSSFLTASPRLMEPVYMVEVHAPADSLPIIYDLLTRRRGHVLQDIPRP 877

Query: 182  GTPMFIVKAYLPVNESFGFTADLR 253
            G+P+++V+A +PV +S GF  DLR
Sbjct: 878  GSPLYLVRALIPVIDSCGFETDLR 901



 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 18/29 (62%), Positives = 21/29 (72%)
 Frame = +1

Query: 256 NTGGQAFPQCVFDHWQVLPGDPCEPHSKP 342
           +T GQA  Q VFDHWQV+PGDP +   KP
Sbjct: 903 HTQGQAMCQMVFDHWQVVPGDPLDKSIKP 931


>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1000

 Score = 78.6 bits (185), Expect = 6e-16
 Identities = 35/82 (42%), Positives = 50/82 (60%)
 Frame = +2

Query: 5    GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 184
            GQ+I   +  +    L   PRLM  +Y C++Q     +G +YGV+++RRG V +E    G
Sbjct: 841  GQVISVVKESIRHGFLGWSPRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEG 900

Query: 185  TPMFIVKAYLPVNESFGFTADL 250
            TP FIVKA +PV ESFGF  ++
Sbjct: 901  TPFFIVKALIPVVESFGFAVEI 922


>SPBC1306.01c ||SPBC409.22c|translation elongation factor
           G|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 770

 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 19/69 (27%), Positives = 37/69 (53%)
 Frame = +2

Query: 50  LTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNES 229
           L A P ++EP+    I  P    GG+ G L++R+  + +         F ++A +P+N  
Sbjct: 668 LQANPMVLEPIMNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSM 725

Query: 230 FGFTADLRS 256
           F +++D+R+
Sbjct: 726 FSYSSDIRA 734


>SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit
           1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 232

 Score = 27.1 bits (57), Expect = 2.0
 Identities = 12/27 (44%), Positives = 15/27 (55%)
 Frame = +1

Query: 295 HWQVLPGDPCEPHSKPYNVVQVTRKRK 375
           H+Q L GDP    + PY  VQ+   RK
Sbjct: 78  HFQNLSGDPVSQMATPYPPVQIELMRK 104


>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = +2

Query: 239 TADLRSTPADRPSRSAYSTIGRSSLETRANLTASPTTL 352
           T+ + ST +   + SA STI +   +T  +++  PTT+
Sbjct: 352 TSSISSTSSSSSTSSAISTISQDHTKTVTSVSDEPTTI 389


>SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 542

 Score = 25.4 bits (53), Expect = 6.1
 Identities = 17/67 (25%), Positives = 29/67 (43%), Gaps = 1/67 (1%)
 Frame = +2

Query: 14  IPTTRRCLYACLLTAQPRLMEPVYL-CEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTP 190
           IP T RC+ +  + + P+L  P  L   + CP +    +  V+     H F    +   P
Sbjct: 386 IPNTERCILSAFICSPPQLPLPNPLRMYLPCPSLNSTEV-SVITLAPQHSFLNIVINLNP 444

Query: 191 MFIVKAY 211
              +K+Y
Sbjct: 445 ALALKSY 451


>SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1032

 Score = 25.4 bits (53), Expect = 6.1
 Identities = 15/43 (34%), Positives = 22/43 (51%)
 Frame = +2

Query: 206  AYLPVNESFGFTADLRSTPADRPSRSAYSTIGRSSLETRANLT 334
            A L +N  F F    ++TPAD  + ++ S    +S E   NLT
Sbjct: 924  ATLILNGIFAFHPPPKATPADSGTETSNSAAFSASEEETTNLT 966


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,399,869
Number of Sequences: 5004
Number of extensions: 49091
Number of successful extensions: 105
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 104
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 250133048
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -