BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0887 (581 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 127 6e-30 At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 103 1e-22 At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 103 1e-22 At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 100 6e-22 At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 74 6e-14 At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 48 3e-06 At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 48 3e-06 At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 45 4e-05 At5g13650.2 68418.m01585 elongation factor family protein contai... 33 0.11 At5g13650.1 68418.m01584 elongation factor family protein contai... 33 0.11 At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634... 29 2.3 At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 6.9 At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, p... 27 6.9 At2g40070.1 68415.m04923 expressed protein 27 9.1 >At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, putative similar to ELONGATION FACTOR 2 GB:O14460 from [Schizosaccharomyces pombe] Length = 843 Score = 127 bits (306), Expect = 6e-30 Identities = 56/85 (65%), Positives = 71/85 (83%) Frame = +2 Query: 2 GGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVA 181 GGQ+IPT RR +YA +TA+PRL+EPVY+ EIQ PE A+GGIY VLN++RGHVFEE Q Sbjct: 703 GGQVIPTARRVIYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRP 762 Query: 182 GTPMFIVKAYLPVNESFGFTADLRS 256 GTP++ +KAYLPV ESFGF++ LR+ Sbjct: 763 GTPLYNIKAYLPVVESFGFSSQLRA 787 Score = 56.8 bits (131), Expect = 1e-08 Identities = 24/42 (57%), Positives = 30/42 (71%) Frame = +1 Query: 259 TGGQAFPQCVFDHWQVLPGDPCEPHSKPYNVVQVTRKRKRLK 384 T GQAFPQCVFDHW+++ DP EP ++ +V RKRK LK Sbjct: 789 TSGQAFPQCVFDHWEMMSSDPLEPGTQASVLVADIRKRKGLK 830 >At1g06220.2 68414.m00656 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 103 bits (246), Expect = 1e-22 Identities = 51/83 (61%), Positives = 57/83 (68%) Frame = +2 Query: 5 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 184 GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV + G Sbjct: 820 GQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPG 879 Query: 185 TPMFIVKAYLPVNESFGFTADLR 253 TP +IVKA+LPV ESFGF DLR Sbjct: 880 TPAYIVKAFLPVIESFGFETDLR 902 Score = 39.1 bits (87), Expect = 0.002 Identities = 14/25 (56%), Positives = 19/25 (76%) Frame = +1 Query: 253 FNTGGQAFPQCVFDHWQVLPGDPCE 327 ++T GQAF VFDHW ++PGDP + Sbjct: 903 YHTQGQAFCLSVFDHWAIVPGDPLD 927 >At1g06220.1 68414.m00655 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 103 bits (246), Expect = 1e-22 Identities = 51/83 (61%), Positives = 57/83 (68%) Frame = +2 Query: 5 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 184 GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV + G Sbjct: 820 GQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPG 879 Query: 185 TPMFIVKAYLPVNESFGFTADLR 253 TP +IVKA+LPV ESFGF DLR Sbjct: 880 TPAYIVKAFLPVIESFGFETDLR 902 Score = 39.1 bits (87), Expect = 0.002 Identities = 14/25 (56%), Positives = 19/25 (76%) Frame = +1 Query: 253 FNTGGQAFPQCVFDHWQVLPGDPCE 327 ++T GQAF VFDHW ++PGDP + Sbjct: 903 YHTQGQAFCLSVFDHWAIVPGDPLD 927 >At5g25230.1 68418.m02991 elongation factor Tu family protein translation Elongation Factor 2, Schizosaccharomyces pombe, PIR:T39902 Length = 973 Score = 100 bits (240), Expect = 6e-22 Identities = 50/83 (60%), Positives = 57/83 (68%) Frame = +2 Query: 5 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 184 GQ+IPT RR Y+ L A PRLMEPVY EIQ P V IY VL+RRRG+V + G Sbjct: 806 GQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPG 865 Query: 185 TPMFIVKAYLPVNESFGFTADLR 253 TP +IVKA+LPV ESFGF DLR Sbjct: 866 TPAYIVKAFLPVIESFGFETDLR 888 Score = 39.1 bits (87), Expect = 0.002 Identities = 14/25 (56%), Positives = 19/25 (76%) Frame = +1 Query: 253 FNTGGQAFPQCVFDHWQVLPGDPCE 327 ++T GQAF VFDHW ++PGDP + Sbjct: 889 YHTQGQAFCLSVFDHWAIVPGDPLD 913 >At3g22980.1 68416.m02898 elongation factor Tu family protein similar to eukaryotic translation elongation factor 2 GB:NP_001952 [Homo sapiens] Length = 1015 Score = 74.1 bits (174), Expect = 6e-14 Identities = 32/83 (38%), Positives = 50/83 (60%) Frame = +2 Query: 5 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 184 GQ++ + A +L PR++E +Y CE+ +G +Y VL+RRR + +E G Sbjct: 853 GQVMTAVKDACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEG 912 Query: 185 TPMFIVKAYLPVNESFGFTADLR 253 + +F V AY+PV+ESFGF +LR Sbjct: 913 SSLFTVHAYVPVSESFGFADELR 935 >At2g45030.1 68415.m05606 mitochondrial elongation factor, putative similar to SP|P25039 Elongation factor G 1, mitochondrial precursor (mEF-G-1) {Saccharomyces cerevisiae}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03764: Elongation factor G domain IV, PF00679: Elongation factor G C-terminus Length = 754 Score = 48.4 bits (110), Expect = 3e-06 Identities = 23/71 (32%), Positives = 39/71 (54%) Frame = +2 Query: 44 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 223 C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706 Query: 224 ESFGFTADLRS 256 FG++ LRS Sbjct: 707 NMFGYSTSLRS 717 >At1g45332.1 68414.m05195 mitochondrial elongation factor, putative similar to mitochondrial elongation factor GI:3917 from [Saccharomyces cerevisiae] Length = 754 Score = 48.4 bits (110), Expect = 3e-06 Identities = 23/71 (32%), Positives = 39/71 (54%) Frame = +2 Query: 44 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 223 C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706 Query: 224 ESFGFTADLRS 256 FG++ LRS Sbjct: 707 NMFGYSTSLRS 717 >At1g62750.1 68414.m07082 elongation factor Tu family protein similar to elongation factor G SP:P34811 [Glycine max (Soybean)] Length = 783 Score = 44.8 bits (101), Expect = 4e-05 Identities = 24/74 (32%), Positives = 38/74 (51%) Frame = +2 Query: 56 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 235 A PR++EP+ E+ PE +G + G LN RRG + G + +V + +P+ E F Sbjct: 683 AGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQ 741 Query: 236 FTADLRSTPADRPS 277 + + LR R S Sbjct: 742 YVSTLRGMTKGRAS 755 >At5g13650.2 68418.m01585 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 676 Score = 33.5 bits (73), Expect = 0.11 Identities = 14/57 (24%), Positives = 28/57 (49%) Frame = +2 Query: 65 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 235 +L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLG 529 >At5g13650.1 68418.m01584 elongation factor family protein contains Pfam profiles: PF00009 elongation factor Tu GTP binding domain,PF00679 elongation factor G C-terminus, PF03144 elongation factor Tu domain 2 Length = 675 Score = 33.5 bits (73), Expect = 0.11 Identities = 14/57 (24%), Positives = 28/57 (49%) Frame = +2 Query: 65 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 235 +L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLG 528 >At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634: Arabidopsis thaliana protein of unknown function (DUF794) Length = 340 Score = 29.1 bits (62), Expect = 2.3 Identities = 11/18 (61%), Positives = 12/18 (66%) Frame = -2 Query: 361 LPVQRCRACCEVRTGLQG 308 +PV RCR C EV G QG Sbjct: 61 VPVHRCRLCAEVHIGKQG 78 >At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein weak similarity to polygalacturonase PG1 [Glycine max] GI:5669846; contains PF00295: Glycosyl hydrolases family 28 Length = 444 Score = 27.5 bits (58), Expect = 6.9 Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%) Frame = +1 Query: 148 SWSRFRRVPGGRY-TYVHCEGL 210 S+ R R +PGGRY +++H +GL Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144 >At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, putative similar to plastid-lipid associated protein PAP3 [Brassica rapa] GI:14248552; contains Pfam profile PF04755: PAP_fibrillin Length = 376 Score = 27.5 bits (58), Expect = 6.9 Identities = 12/22 (54%), Positives = 13/22 (59%) Frame = +1 Query: 286 VFDHWQVLPGDPCEPHSKPYNV 351 V D W PGD EP S+P NV Sbjct: 71 VTDEWGEKPGDANEPDSQPDNV 92 >At2g40070.1 68415.m04923 expressed protein Length = 607 Score = 27.1 bits (57), Expect = 9.1 Identities = 16/35 (45%), Positives = 20/35 (57%), Gaps = 3/35 (8%) Frame = +2 Query: 254 STPADRPSRSAYSTIGRSSLETR---ANLTASPTT 349 STP RP+ TI RSS TR A+ +A+ TT Sbjct: 296 STPTSRPTLPPSKTISRSSTPTRRPIASASAATTT 330 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,618,573 Number of Sequences: 28952 Number of extensions: 261252 Number of successful extensions: 604 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 588 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 602 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1141585696 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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