BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0867 (612 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 125 6e-30 SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 123 2e-29 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 100 2e-22 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 82 7e-17 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 77 2e-15 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 33 0.043 SPBC16C6.05 |||translation initiation factor |Schizosaccharomyce... 31 0.099 SPAC23H4.15 |||ribosome biogenesis protein Tsr1 |Schizosaccharom... 31 0.17 SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosacch... 29 0.53 SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyc... 28 0.93 SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1 |Sc... 28 1.2 SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces po... 27 1.6 SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo... 27 2.1 SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch... 27 2.8 SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 27 2.8 SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 27 2.8 SPCC613.05c |rpl35||60S ribosomal protein L35|Schizosaccharomyce... 26 3.7 SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schi... 26 3.7 SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 26 4.9 SPBC146.06c |||human MTMR15 homolog|Schizosaccharomyces pombe|ch... 26 4.9 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 26 4.9 SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145 |Schizo... 25 6.5 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 6.5 SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 25 6.5 SPAC1071.05 |||S-adenosylmethionine-dependent methyltransferase ... 25 8.6 SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 25 8.6 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 125 bits (301), Expect = 6e-30 Identities = 57/85 (67%), Positives = 72/85 (84%) Frame = +3 Query: 3 EVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKE 182 EVTFD+DANGILNVSA+EK T K KITITNDKGRLSKEEI+RMV+EAEKY+ ED+ + Sbjct: 473 EVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEIDRMVSEAEKYKAEDEAETS 532 Query: 183 TIQAKNALESYCFSMKSTMEDEKLR 257 IQAKN LESY +S++++++D L+ Sbjct: 533 RIQAKNHLESYAYSLRNSLDDPNLK 557 Score = 66.9 bits (156), Expect = 2e-12 Identities = 29/54 (53%), Positives = 39/54 (72%) Frame = +2 Query: 254 QEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 415 ++K+ SDK+ I +TI+WLD N A K+EYE KQKELEG+ NPI+ K+YQ Sbjct: 557 KDKVDASDKEAIDKAVKETIEWLDHNTTAAKDEYEDKQKELEGVANPIMAKIYQ 610 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 123 bits (297), Expect = 2e-29 Identities = 57/85 (67%), Positives = 71/85 (83%) Frame = +3 Query: 3 EVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKE 182 EVTFD+DANGILNVSA+EK T K KITITNDKGRLSKEEI+RMV EAEKY+ ED+ + Sbjct: 473 EVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEIDRMVAEAEKYKAEDEAESG 532 Query: 183 TIQAKNALESYCFSMKSTMEDEKLR 257 IQAKN LESY +S++++++D L+ Sbjct: 533 RIQAKNHLESYAYSLRNSLDDPNLK 557 Score = 66.1 bits (154), Expect = 4e-12 Identities = 28/54 (51%), Positives = 40/54 (74%) Frame = +2 Query: 254 QEKISDSDKQTILDKCNDTIKWLDSNQLADKEEYEHKQKELEGIYNPIITKMYQ 415 ++K+ SDK+T+ +TI+WLDSN A K+E+E KQKELE + NPI+ K+YQ Sbjct: 557 KDKVDASDKETVDKAVKETIEWLDSNTTAAKDEFEAKQKELESVANPIMAKIYQ 610 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 100 bits (239), Expect = 2e-22 Identities = 46/84 (54%), Positives = 64/84 (76%), Gaps = 1/84 (1%) Frame = +3 Query: 3 EVTFDIDANGILNVSAIEKS-TNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQK 179 EVTF++DANG+L VSA++KS K K+ I NDKGRLS+E+IERMV EAE++ ED K Sbjct: 502 EVTFEVDANGVLTVSAVDKSGKGKPEKLVIKNDKGRLSEEDIERMVKEAEEFAEEDKILK 561 Query: 180 ETIQAKNALESYCFSMKSTMEDEK 251 E I+A+N LE+Y +S+K +D++ Sbjct: 562 ERIEARNTLENYAYSLKGQFDDDE 585 Score = 46.4 bits (105), Expect = 3e-06 Identities = 19/52 (36%), Positives = 34/52 (65%), Gaps = 1/52 (1%) Frame = +2 Query: 260 KISDSDKQTILDKCNDTIKWLDSN-QLADKEEYEHKQKELEGIYNPIITKMY 412 K+ DKQ +LD D +WL+ + + A KEE+E ++++L+ + +PI K+Y Sbjct: 590 KVDPEDKQAVLDAVEDVAEWLEIHGEDASKEEFEDQRQKLDAVVHPITQKLY 641 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 81.8 bits (193), Expect = 7e-17 Identities = 40/83 (48%), Positives = 57/83 (68%) Frame = +3 Query: 3 EVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKE 182 EV+FD+DA+GI+NVSA +K+TNK++ IT+ G L+ EIE MV +AEKYR D +KE Sbjct: 516 EVSFDVDADGIINVSARDKATNKDSSITVAGSSG-LTDSEIEAMVADAEKYRASDMARKE 574 Query: 183 TIQAKNALESYCFSMKSTMEDEK 251 I+ N ES C ++S ++ K Sbjct: 575 AIENGNRAESVCTDIESNLDIHK 597 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 77.0 bits (181), Expect = 2e-15 Identities = 33/81 (40%), Positives = 56/81 (69%) Frame = +3 Query: 3 EVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKE 182 E TF++DANGIL V+A+EK+T + I ITN G LS +I+ M+ A+K++ +D + Sbjct: 478 EATFELDANGILKVTAVEKTTGRSAHIEITNSVGHLSSTKIQEMIENADKFKQQDKDFAK 537 Query: 183 TIQAKNALESYCFSMKSTMED 245 ++AK+ LESY ++++T+ + Sbjct: 538 KLEAKSQLESYISNIETTISE 558 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 32.7 bits (71), Expect = 0.043 Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 1/64 (1%) Frame = +3 Query: 57 KSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQ-KETIQAKNALESYCFSMKS 233 K K ++++ + RL E +E+ EAE DK ET+ KNALE Y + ++ Sbjct: 534 KKLVKVADLSVSVQEDRLPTEVLEKY-REAEHQMIATDKLVAETVDRKNALEEYIYDTRA 592 Query: 234 TMED 245 ++D Sbjct: 593 KLDD 596 >SPBC16C6.05 |||translation initiation factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 190 Score = 31.5 bits (68), Expect = 0.099 Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 2/63 (3%) Frame = +3 Query: 21 DANGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKE--TIQA 194 D LNVS + S +E +T ++ R+ +EE +RM ++ E K+K T+Q Sbjct: 58 DLENTLNVSGTKDSNAEEQPAKLTKEEKRVEREEAKRMASKVLIKTIERTKRKRVTTVQG 117 Query: 195 KNA 203 +A Sbjct: 118 LDA 120 >SPAC23H4.15 |||ribosome biogenesis protein Tsr1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 783 Score = 30.7 bits (66), Expect = 0.17 Identities = 16/44 (36%), Positives = 25/44 (56%), Gaps = 1/44 (2%) Frame = +2 Query: 251 AQEKISDSDKQTILD-KCNDTIKWLDSNQLADKEEYEHKQKELE 379 A+EKI +++TI D K + + ++ EEY KQKEL+ Sbjct: 426 AEEKIDSDEEETIDDAKSEMFVDLSEEEEVRQYEEYRKKQKELQ 469 >SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1362 Score = 29.1 bits (62), Expect = 0.53 Identities = 14/24 (58%), Positives = 16/24 (66%) Frame = +3 Query: 99 KGRLSKEEIERMVNEAEKYRNEDD 170 KG LSKEE+ER V +A K N D Sbjct: 525 KGTLSKEELERRVYDAAKLANAYD 548 >SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 682 Score = 28.3 bits (60), Expect = 0.93 Identities = 18/52 (34%), Positives = 26/52 (50%) Frame = +3 Query: 66 NKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNALESYCF 221 NK+ K T D SKEE+E +V E E D +Q + K+ L++ F Sbjct: 529 NKKGKHTQIEDPTAASKEELENLVREDE----NDSEQLDHFDMKSILKAEKF 576 >SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 474 Score = 27.9 bits (59), Expect = 1.2 Identities = 15/44 (34%), Positives = 22/44 (50%) Frame = +3 Query: 36 LNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNED 167 L VS ++S K + + K EE+E VNE E++R D Sbjct: 257 LCVSPEKRSLAKTREAVLAVTKWTTDDEELESFVNEEEEFRASD 300 >SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 27.5 bits (58), Expect = 1.6 Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 1/36 (2%) Frame = +2 Query: 29 RYPQRFRYREVHQQGEQDHHYQRQRSS-LQGRDRAY 133 R P R +YRE H + + H R QGR +Y Sbjct: 320 RDPDRTKYREYHSERRKQHRTDRYSDDYYQGRSYSY 355 >SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizosaccharomyces pombe|chr 2|||Manual Length = 872 Score = 27.1 bits (57), Expect = 2.1 Identities = 14/35 (40%), Positives = 22/35 (62%) Frame = +3 Query: 132 MVNEAEKYRNEDDKQKETIQAKNALESYCFSMKST 236 M+++ RN+ +KQ TI KNA++ Y S+K T Sbjct: 247 MLSKLVSSRNKPNKQ--TILTKNAIQDYLVSLKIT 279 >SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 509 Score = 26.6 bits (56), Expect = 2.8 Identities = 19/74 (25%), Positives = 33/74 (44%), Gaps = 1/74 (1%) Frame = +3 Query: 27 NGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAE-KYRNEDDKQKETIQAKNA 203 +GIL+ + IEK +K K+ + N+ SK+ + + + +E DK KN Sbjct: 154 DGILSFTVIEKVDDKNLKVRVNNNGKISSKKGVNLPKTDVDLPALSEKDKADLRFGVKNG 213 Query: 204 LESYCFSMKSTMED 245 ++ S ED Sbjct: 214 VDMIFASFIRRAED 227 >SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pombe|chr 2|||Manual Length = 1067 Score = 26.6 bits (56), Expect = 2.8 Identities = 13/54 (24%), Positives = 28/54 (51%) Frame = +3 Query: 42 VSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNA 203 V A+ KS +++ ++ G + EEI ++++ E E+D++ E N+ Sbjct: 597 VPALFKSIDQDESDILSERIGAPTAEEISQLLDSVETNEEENDEELEKAMGVNS 650 >SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizosaccharomyces pombe|chr 1|||Manual Length = 1290 Score = 26.6 bits (56), Expect = 2.8 Identities = 17/58 (29%), Positives = 34/58 (58%), Gaps = 1/58 (1%) Frame = +3 Query: 72 ENKITITNDKGRLSKEEIERM-VNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTME 242 ++++TIT++ S E++E++ N AEK + E + K + ++ LES S + +E Sbjct: 288 KSQMTITDE----SSEDLEKLHSNFAEKVKEEQELYKSLEKKRSDLESLLKSRRELLE 341 >SPCC613.05c |rpl35||60S ribosomal protein L35|Schizosaccharomyces pombe|chr 3|||Manual Length = 122 Score = 26.2 bits (55), Expect = 3.7 Identities = 21/69 (30%), Positives = 35/69 (50%), Gaps = 2/69 (2%) Frame = +3 Query: 57 KSTNKE--NKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMK 230 K+T K+ +T+ N+ RL+ E + +KY D +QK+T + AL Y S K Sbjct: 47 KTTRKDIARILTVINESNRLAAREAYKN----KKYIPLDLRQKKTRAIRRALTPYEQSRK 102 Query: 231 STMEDEKLR 257 + + +K R Sbjct: 103 TLKQIKKER 111 >SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schizosaccharomyces pombe|chr 1|||Manual Length = 695 Score = 26.2 bits (55), Expect = 3.7 Identities = 12/41 (29%), Positives = 25/41 (60%) Frame = +3 Query: 111 SKEEIERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKS 233 S+ E +++ E ++ ED+KQ E + K AL+ +C +++ Sbjct: 299 SETEQQQLFFEYKQKLLEDEKQLEKDRRKEALDDFCSLLRN 339 >SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 764 Score = 25.8 bits (54), Expect = 4.9 Identities = 12/37 (32%), Positives = 17/37 (45%) Frame = +3 Query: 99 KGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNALE 209 +G L E E NEA++ NE+ E +N E Sbjct: 89 EGELKLENFENQENEADEAENEETSYSEQNHTENTEE 125 >SPBC146.06c |||human MTMR15 homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 703 Score = 25.8 bits (54), Expect = 4.9 Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 2/56 (3%) Frame = +3 Query: 60 STNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQK--ETIQAKNALESYCF 221 +TNK N + N L EEI V + E + DD+ + E +A ++ E Y F Sbjct: 46 NTNKHNVYSTKNSMYLLGFEEILNTVLDCEPHIFTDDELRVIENFKALDSDERYLF 101 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 25.8 bits (54), Expect = 4.9 Identities = 14/50 (28%), Positives = 24/50 (48%) Frame = +3 Query: 99 KGRLSKEEIERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTMEDE 248 +GR + + N+ E + DDKQ + KN+LE+ M+ + E Sbjct: 1259 RGRQKTSIVAKYRNKRELPEDSDDKQDTASKDKNSLETIDEKMEDASKIE 1308 >SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145 |Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 25.4 bits (53), Expect = 6.5 Identities = 14/43 (32%), Positives = 23/43 (53%) Frame = +3 Query: 57 KSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKET 185 K NK+++ + +K +L +E+ AEK N DDK + T Sbjct: 39 KQKNKKSRNQVRREKKKLLREKTNSGAKLAEK--NSDDKDQLT 79 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 25.4 bits (53), Expect = 6.5 Identities = 14/47 (29%), Positives = 23/47 (48%) Frame = +3 Query: 27 NGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNED 167 N + N S +KS+NKE + + RLS + + K ++ED Sbjct: 409 NTVQNESKSQKSSNKEGRSSRGGFFSRLSFSRSSSRMKKGSKAKHED 455 >SPCC162.08c |nup211||nuclear pore complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1837 Score = 25.4 bits (53), Expect = 6.5 Identities = 14/50 (28%), Positives = 29/50 (58%) Frame = +3 Query: 111 SKEEIERMVNEAEKYRNEDDKQKETIQAKNALESYCFSMKSTMEDEKLRK 260 +KEE+E +NE + E ++Q + ++N ++ +K +E EKL++ Sbjct: 1606 TKEELENQLNEKSQRIKELEEQAQKNSSENTHDNIDDMIKQQVE-EKLKE 1654 >SPAC1071.05 |||S-adenosylmethionine-dependent methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 339 Score = 25.0 bits (52), Expect = 8.6 Identities = 9/22 (40%), Positives = 12/22 (54%) Frame = +2 Query: 248 EAQEKISDSDKQTILDKCNDTI 313 E + I DK +LD CND + Sbjct: 74 EQDDSIESDDKLKVLDGCNDLV 95 >SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccharomyces pombe|chr 3|||Manual Length = 1284 Score = 25.0 bits (52), Expect = 8.6 Identities = 23/73 (31%), Positives = 32/73 (43%), Gaps = 10/73 (13%) Frame = +3 Query: 15 DIDANGILNVSAIEKSTNKENKIT----ITNDKGRLSKEEIER-----MVNEAE-KYRNE 164 D+D VS IEK+T + +T N G+L K EIE ++N+ E Sbjct: 1209 DLDTEASPVVSTIEKTTKGDVSVTEEQQSANIDGQLEKPEIEESKKPDVLNQVSLSIEEE 1268 Query: 165 DDKQKETIQAKNA 203 K KE+ NA Sbjct: 1269 KPKNKESEVDNNA 1281 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,076,258 Number of Sequences: 5004 Number of extensions: 35672 Number of successful extensions: 201 Number of sequences better than 10.0: 26 Number of HSP's better than 10.0 without gapping: 187 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 198 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 267622334 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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