BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0854 (558 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 29 0.35 SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3... 27 1.9 SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 2.5 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 26 4.3 SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 25 5.7 SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 25 5.7 SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharom... 25 7.5 SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|c... 25 7.5 SPBC21D10.10 |||bromodomain protein|Schizosaccharomyces pombe|ch... 25 10.0 SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosacc... 25 10.0 SPBC4F6.10 |vps901|vps9a|guanyl-nucleotide exchange factor Vps90... 25 10.0 >SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces pombe|chr 2|||Manual Length = 811 Score = 29.5 bits (63), Expect = 0.35 Identities = 26/80 (32%), Positives = 37/80 (46%), Gaps = 1/80 (1%) Frame = +1 Query: 259 ALENVVAEATASVSLADNIIVSDIGAAITIGDVKSNLQINLFGREVGPAVNNFLEKIPVY 438 A E +A S L N+ + AAIT K + + F +E G V E+I Sbjct: 74 AHEAFIASKIVSGDLKTNL---QVNAAITYCKDKDTIDESEFDKETGVGVVLTPEQIEQL 130 Query: 439 LTDYAAE-VSRVLEYVAQLV 495 + DY AE S++LE QL+ Sbjct: 131 VGDYVAENKSKILEQRYQLL 150 >SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 449 Score = 27.1 bits (57), Expect = 1.9 Identities = 14/40 (35%), Positives = 25/40 (62%), Gaps = 1/40 (2%) Frame = +1 Query: 337 AITIGDVKSNLQINLFGREVGPAVNNFL-EKIPVYLTDYA 453 AITIGD + L + +++GP V +F + +P +LT ++ Sbjct: 178 AITIGDNSTLLYTSWLFQKIGPPVLSFSDDDVPGFLTHFS 217 >SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2100 Score = 26.6 bits (56), Expect = 2.5 Identities = 18/82 (21%), Positives = 37/82 (45%), Gaps = 2/82 (2%) Frame = -1 Query: 333 SDVTDNDVVCEGDGSGSLSNDVLKGKSPEAKDLRTRLKMRISPSAEPTD--AETLGTART 160 S++ N +V + D L DVL + P+ D+ T+ + S TD A+ G + Sbjct: 1070 SEMYRNHLVQKADLEKELPEDVLNAEFPDGTDVITQYIVAASSQITVTDAVAQVFGKPKP 1129 Query: 159 KLSRVPT*LTRRKCRELKSSAL 94 + + + ++ E+ S+ + Sbjct: 1130 AIKNITQLIIQQSVLEIISAVV 1151 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 25.8 bits (54), Expect = 4.3 Identities = 28/86 (32%), Positives = 44/86 (51%), Gaps = 4/86 (4%) Frame = +1 Query: 247 FRRLALENVVAEATASVSLADNIIVSDIGAAITIGDVKSNLQINLFGREV----GPAVNN 414 +RR L+ V+A A S D VS + A+ D+ S L I L + V + N Sbjct: 962 YRRPLLDQVIATAVPESS--DPEAVSIVVKALMEVDLPSQL-IELLEKIVLQPSSFSENA 1018 Query: 415 FLEKIPVYLTDYAAEVSRVLEYVAQL 492 L+ + ++LT A+ SRV+EY+ +L Sbjct: 1019 NLQNL-LFLTAIKADKSRVMEYIDKL 1043 >SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1106 Score = 25.4 bits (53), Expect = 5.7 Identities = 10/30 (33%), Positives = 16/30 (53%) Frame = +1 Query: 424 KIPVYLTDYAAEVSRVLEYVAQLVINRLRK 513 K+P + DY EV R+++ A L + K Sbjct: 968 KVPTFYADYVNEVKRIIQRNANLEFEAIWK 997 >SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1935 Score = 25.4 bits (53), Expect = 5.7 Identities = 12/41 (29%), Positives = 24/41 (58%) Frame = +1 Query: 379 LFGREVGPAVNNFLEKIPVYLTDYAAEVSRVLEYVAQLVIN 501 + R++GP V F+ K+P L + ++ + + V +LV+N Sbjct: 972 IHNRKMGPTVKKFISKLP--LLNINVDLLPLTKNVLRLVLN 1010 >SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 500 Score = 25.0 bits (52), Expect = 7.5 Identities = 9/14 (64%), Positives = 11/14 (78%) Frame = -2 Query: 386 PKRLIWRLDLTSPI 345 PK LIWR LT+P+ Sbjct: 436 PKELIWRSRLTNPV 449 >SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 708 Score = 25.0 bits (52), Expect = 7.5 Identities = 10/36 (27%), Positives = 20/36 (55%) Frame = +1 Query: 211 NPHFQPRPEVFRFRRLALENVVAEATASVSLADNII 318 +P ++P ++ R+ +L V EAT S + + +I Sbjct: 276 DPAYEPHKQIARYEKLQQIFQVVEATVSPEVGERLI 311 >SPBC21D10.10 |||bromodomain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 299 Score = 24.6 bits (51), Expect = 10.0 Identities = 18/88 (20%), Positives = 41/88 (46%) Frame = -1 Query: 483 DVLQNTADLGCVVRQVDRDLL*EVVDGWSNFASEEIDLEVRFDIANSYCSSDVTDNDVVC 304 ++ TA+ ++ +++D L ++V+ W + +++ + S+ V+ ND Sbjct: 26 ELFNRTAESSLLLEPIEQDQLKDLVEEWMHDSNKS---------DSKLLSNQVSQNDNDA 76 Query: 303 EGDGSGSLSNDVLKGKSPEAKDLRTRLK 220 + L NDVLK + E + +L+ Sbjct: 77 RKNLRKRLRNDVLKDLAEEIQGCEKKLE 104 >SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 24.6 bits (51), Expect = 10.0 Identities = 10/24 (41%), Positives = 13/24 (54%) Frame = +2 Query: 479 TSPNSSSTDYVNYSNDTCESKINY 550 TSPN S T NY+ D + +Y Sbjct: 214 TSPNQSHTSLANYNPDLLDGPPDY 237 >SPBC4F6.10 |vps901|vps9a|guanyl-nucleotide exchange factor Vps901 |Schizosaccharomyces pombe|chr 2|||Manual Length = 537 Score = 24.6 bits (51), Expect = 10.0 Identities = 14/48 (29%), Positives = 25/48 (52%) Frame = +1 Query: 364 NLQINLFGREVGPAVNNFLEKIPVYLTDYAAEVSRVLEYVAQLVINRL 507 N Q+ L R+ +N +E+ + + AE+ E + +LV+NRL Sbjct: 149 NYQVKLI-RDFLKFINEKIEQYEPWASGSQAEIDNAKEGMEKLVLNRL 195 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,946,096 Number of Sequences: 5004 Number of extensions: 34586 Number of successful extensions: 124 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 121 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 123 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 233995432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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