BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0838
(631 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces... 111 6e-26
SPCC1919.03c |||AMP-activated protein kinase beta subunit |Schiz... 26 3.9
SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces... 26 3.9
SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo... 25 6.8
SPAC3A12.15 |vps53||GARP complex subunit Vps53 |Schizosaccharomy... 25 6.8
SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr... 25 9.0
SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|... 25 9.0
>SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 195
Score = 111 bits (268), Expect = 6e-26
Identities = 53/88 (60%), Positives = 66/88 (75%)
Frame = +3
Query: 255 ELEKKFSGKHVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVYNAILEDLVFPAEIVG 434
ELEKKF+ +HV+F+ R+ILPKP K+RV QKRPRSRTLT+V+NAILED+VFP EI+G
Sbjct: 82 ELEKKFADRHVIFIAQRRILPKPGRKSRVT--QKRPRSRTLTAVHNAILEDIVFPTEIIG 139
Query: 435 KRIRVKLDGSQLIKVHLDKNQQTTMNIK 518
KR R DG + IKV LD T++ K
Sbjct: 140 KRTRQATDGRKTIKVFLDNRDANTVDYK 167
Score = 60.1 bits (139), Expect = 3e-10
Identities = 30/71 (42%), Positives = 48/71 (67%), Gaps = 2/71 (2%)
Frame = +1
Query: 34 KIIKASGAEADSFETSISQALVELETNS-DLKAQLRELYITKAKEIELHN-KKSIIIYVP 207
KI+K S ++ + ++Q L +LE++S D+ +LR L IT A+E+E+ KK+I+++VP
Sbjct: 6 KIVKRSSSQPTETDLLVAQCLYDLESSSKDMAKELRPLQITSAREVEVGGGKKAIVVFVP 65
Query: 208 MPKLKAFQKIQ 240
P LKAF K Q
Sbjct: 66 QPLLKAFHKCQ 76
Score = 38.7 bits (86), Expect = 7e-04
Identities = 16/33 (48%), Positives = 23/33 (69%)
Frame = +2
Query: 482 LRQKPTDYYEHKVDTFQSVYKKLTGREVTFEFP 580
L + + ++K+ +F SVY KLTG+ VTFEFP
Sbjct: 156 LDNRDANTVDYKLGSFSSVYHKLTGKNVTFEFP 188
>SPCC1919.03c |||AMP-activated protein kinase beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 298
Score = 26.2 bits (55), Expect = 3.9
Identities = 10/31 (32%), Positives = 15/31 (48%)
Frame = +3
Query: 237 PDQACPELEKKFSGKHVVFVGDRKILPKPSH 329
P P LEK + + D+ +LP P+H
Sbjct: 229 PPSLPPHLEKCILNSNTAYKEDQSVLPNPNH 259
>SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 404
Score = 26.2 bits (55), Expect = 3.9
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = +2
Query: 353 KEATLKDIDLCVQCYPRGLGLPC 421
++AT++++D C C RGL + C
Sbjct: 110 RKATIRNVDYCSACGGRGLFICC 132
>SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3
Rfp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 254
Score = 25.4 bits (53), Expect = 6.8
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +3
Query: 315 PKPSHKTRVANKQKRPRSRTLTSVYNAILEDLVFPAEIVGKR 440
P S + R N+++ RSR S + + LED+++ V R
Sbjct: 49 PVLSPRRRRMNRRRNERSRNFPSNHLSYLEDMIYLGPQVSTR 90
>SPAC3A12.15 |vps53||GARP complex subunit Vps53 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 756
Score = 25.4 bits (53), Expect = 6.8
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +2
Query: 503 YYEHKVDTFQSVYKKLTG 556
+YE DTFQS +KK +G
Sbjct: 188 FYEQVFDTFQSQFKKESG 205
>SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 703
Score = 25.0 bits (52), Expect = 9.0
Identities = 11/26 (42%), Positives = 19/26 (73%)
Frame = -3
Query: 371 P*AWPLLFVSNTSFVAGLRQDLTVSN 294
P A +LF+S TSF++G+ Q + ++N
Sbjct: 373 PPAAMILFISCTSFISGILQLVLLNN 398
>SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 532
Score = 25.0 bits (52), Expect = 9.0
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -1
Query: 502 VCWFLSKCTLMSCEPSNLTLMR 437
+C FL K T SC NL L++
Sbjct: 207 LCSFLPKSTYRSCRSDNLDLLK 228
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,392,868
Number of Sequences: 5004
Number of extensions: 46080
Number of successful extensions: 146
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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