Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0827
         (760 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical...    67   1e-11
At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) ...    66   2e-11
At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) ...    54   1e-07
At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) ...    44   1e-04
At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 ...    39   0.003

>At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to
           Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis
           thaliana]; identical to cDNA SMT3 protein GI:1707371
          Length = 100

 Score = 66.9 bits (156), Expect = 1e-11
 Identities = 32/58 (55%), Positives = 40/58 (68%), Gaps = 1/58 (1%)
 Frame = +3

Query: 78  DEKKGEN-EHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQ 248
           D+K G+   HINLKV GQD   V F+IK+ T L+KLMNAYCDR  + M  + F FDG+
Sbjct: 8   DKKPGDGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNSIAFLFDGR 65



 Score = 30.3 bits (65), Expect = 1.5
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = +2

Query: 266 TPTSLEMEEGDTIEVYQQQTGG 331
           TP  L+ME+GD I+    QTGG
Sbjct: 72  TPDELDMEDGDEIDAMLHQTGG 93


>At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852 from
           [Arabidopsis thaliana]; identical to cDNA small
           ubiquitin-like modifier 2 (SUMO)  GI:22652843; contains
           Pfam profile PF00240: Ubiquitin family
          Length = 103

 Score = 66.5 bits (155), Expect = 2e-11
 Identities = 30/57 (52%), Positives = 38/57 (66%)
 Frame = +3

Query: 78  DEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQ 248
           D+K  +  HINLKV GQD   V F+IK+ T L+KLMNAYCDR  +    + F FDG+
Sbjct: 8   DKKPDQGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDFNSIAFLFDGR 64



 Score = 31.5 bits (68), Expect = 0.63
 Identities = 13/22 (59%), Positives = 15/22 (68%)
 Frame = +2

Query: 266 TPTSLEMEEGDTIEVYQQQTGG 331
           TP  LEME+GD I+    QTGG
Sbjct: 71  TPDELEMEDGDEIDAMLHQTGG 92


>At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO)
           similar to SP|O13351 Ubiquitin-like protein smt3/pmt3
           {Schizosaccharomyces pombe}; identical to cDNA small
           ubiquitin-like modifier 3 (SUMO) GI:22652845
          Length = 111

 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 26/54 (48%), Positives = 31/54 (57%)
 Frame = +3

Query: 93  ENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPI 254
           +  H+ LKV  QD   V FK KK  PL+KLM  YCDR GL +    F F+G  I
Sbjct: 14  QEAHVILKVKSQDGDEVLFKNKKSAPLKKLMYVYCDRRGLKLDAFAFIFNGARI 67



 Score = 28.7 bits (61), Expect = 4.4
 Identities = 10/24 (41%), Positives = 17/24 (70%)
 Frame = +2

Query: 263 DTPTSLEMEEGDTIEVYQQQTGGV 334
           +TP  L+ME+GD I+  +  +GG+
Sbjct: 71  ETPDELDMEDGDVIDACRAMSGGL 94


>At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO)
           similar to ubiquitin-like protein SMT3 SP:P55852
           [Arabidopsis thaliana]; contains INTERPRO:IPR000626
           ubiquitin domain; contains Pfam profile PF00240:
           Ubiquitin family; contains Pfam profile PF00240:
           Ubiquitin family;  identical to cDNA small
           ubiquitin-like modifier 5 (SUMO) mRNA GI:22652847
          Length = 108

 Score = 44.0 bits (99), Expect = 1e-04
 Identities = 22/65 (33%), Positives = 34/65 (52%)
 Frame = +3

Query: 60  INLKMADEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRF 239
           ++ K    +   +  + LKV  Q  A   +KI  H  L+KLM+AYC +  L    VRF +
Sbjct: 13  VSKKSRSPETSPHMKVTLKVKNQQGAEDLYKIGTHAHLKKLMSAYCTKRNLDYSSVRFVY 72

Query: 240 DGQPI 254
           +G+ I
Sbjct: 73  NGREI 77


>At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351
           Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces
           pombe}
          Length = 114

 Score = 39.1 bits (87), Expect = 0.003
 Identities = 22/67 (32%), Positives = 34/67 (50%), Gaps = 4/67 (5%)
 Frame = +3

Query: 66  LKMADEKK---GENEHINLKVLGQDNAIVQ-FKIKKHTPLRKLMNAYCDRAGLSMQVVRF 233
           +KM  +K+    +  H+ LKV GQD    + F ++++  L K+M  Y    G+     RF
Sbjct: 12  VKMEGQKRKVVSDPTHVTLKVKGQDEEDFRVFWVRRNAKLLKMMELYTKMRGIEWNTFRF 71

Query: 234 RFDGQPI 254
            FDG  I
Sbjct: 72  LFDGSRI 78


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,294,000
Number of Sequences: 28952
Number of extensions: 298105
Number of successful extensions: 600
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 580
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 600
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1692519896
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)