Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0814
(470 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 125 2e-29
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 124 2e-29
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 124 2e-29
At1g67870.1 68414.m07750 glycine-rich protein contains non-conse... 33 0.098
At1g31817.1 68414.m03907 chloroplast 30S ribosomal protein S11, ... 31 0.30
At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3 d... 29 2.1
At1g68670.1 68414.m07846 myb family transcription factor contain... 29 2.1
At1g14210.1 68414.m01682 ribonuclease T2 family protein contains... 29 2.1
At2g26730.1 68415.m03206 leucine-rich repeat transmembrane prote... 28 3.7
At3g45570.1 68416.m04922 zinc finger (C3HC4-type RING finger) fa... 27 4.8
At2g38570.1 68415.m04738 expressed protein ; expression supporte... 27 4.8
At5g60250.1 68418.m07552 zinc finger (C3HC4-type RING finger) fa... 27 6.4
At3g45580.1 68416.m04923 zinc finger (C3HC4-type RING finger) fa... 27 6.4
At3g22330.1 68416.m02820 DEAD box RNA helicase, putative similar... 27 6.4
At3g63100.1 68416.m07087 glycine-rich protein 27 8.5
At3g45555.1 68416.m04920 zinc finger (C3HC4-type RING finger) fa... 27 8.5
At2g40760.1 68415.m05028 rhodanese-like domain-containing protei... 27 8.5
At2g28130.1 68415.m03417 expressed protein 27 8.5
At1g62730.1 68414.m07080 expressed protein 27 8.5
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 125 bits (301), Expect = 2e-29
Identities = 54/84 (64%), Positives = 69/84 (82%)
Frame = +2
Query: 2 EAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFES 181
EAK+KLCKV+ + G K +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+
Sbjct: 118 EAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEANKIVEFIKFDV 177
Query: 182 GNLCMITGGRNLGRVGTIVSRERH 253
GN+ M+TGGRN GRVG I +RE+H
Sbjct: 178 GNVVMVTGGRNRGRVGVIKNREKH 201
Score = 88.2 bits (209), Expect = 2e-18
Identities = 38/57 (66%), Positives = 49/57 (85%)
Frame = +1
Query: 256 GSFDIVHIKDSTGHTFATRLNNVFIIGKGTKAYISLPRGKGIRLTIAEERDKRIAAK 426
GSF+ +HI+DSTGH FATRL NV+ IGKGTK ++SLP+GKGI+LTI EE KR+A++
Sbjct: 203 GSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKGIKLTIIEEARKRLASQ 259
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 124 bits (300), Expect = 2e-29
Identities = 54/84 (64%), Positives = 69/84 (82%)
Frame = +2
Query: 2 EAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFES 181
EAK+KLCKV+ + G K +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+
Sbjct: 118 EAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDV 177
Query: 182 GNLCMITGGRNLGRVGTIVSRERH 253
GN+ M+TGGRN GRVG I +RE+H
Sbjct: 178 GNVVMVTGGRNRGRVGVIKNREKH 201
Score = 88.2 bits (209), Expect = 2e-18
Identities = 38/57 (66%), Positives = 49/57 (85%)
Frame = +1
Query: 256 GSFDIVHIKDSTGHTFATRLNNVFIIGKGTKAYISLPRGKGIRLTIAEERDKRIAAK 426
GSF+ +HI+DSTGH FATRL NV+ IGKGTK ++SLP+GKGI+LTI EE KR+A++
Sbjct: 203 GSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKGIKLTIIEEARKRLASQ 259
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 124 bits (300), Expect = 2e-29
Identities = 54/84 (64%), Positives = 69/84 (82%)
Frame = +2
Query: 2 EAKYKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFES 181
EAK+KLCKV+ + G K +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+
Sbjct: 118 EAKFKLCKVRSIQFGQKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDV 177
Query: 182 GNLCMITGGRNLGRVGTIVSRERH 253
GN+ M+TGGRN GRVG I +RE+H
Sbjct: 178 GNVVMVTGGRNRGRVGVIKNREKH 201
Score = 88.2 bits (209), Expect = 2e-18
Identities = 38/57 (66%), Positives = 49/57 (85%)
Frame = +1
Query: 256 GSFDIVHIKDSTGHTFATRLNNVFIIGKGTKAYISLPRGKGIRLTIAEERDKRIAAK 426
GSF+ +HI+DSTGH FATRL NV+ IGKGTK ++SLP+GKGI+LTI EE KR++A+
Sbjct: 203 GSFETIHIQDSTGHEFATRLGNVYTIGKGTKPWVSLPKGKGIKLTIIEEARKRLSAQ 259
>At1g67870.1 68414.m07750 glycine-rich protein contains
non-consensus GG donor splice site at exon2; modeled to
est match.
Length = 279
Score = 33.1 bits (72), Expect = 0.098
Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 2/50 (4%)
Frame = +3
Query: 276 HQGLHGTHLR--HEVEQRVHNRQGHEGVHLAAARQGHPPHHRRGAGQAHR 419
HQG+HG + H ++ + + H+G+H + GH H+ G G H+
Sbjct: 154 HQGMHGMQHQGGHGMQHQGGHGMQHQGMHGMQHQGGHGMEHQGGHGMQHQ 203
Score = 31.9 bits (69), Expect = 0.23
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 2/50 (4%)
Frame = +3
Query: 276 HQGLHGT--HLRHEVEQRVHNRQGHEGVHLAAARQGHPPHHRRGAGQAHR 419
HQG HG H H ++ + + H+G+H + GH H+ G G H+
Sbjct: 130 HQGGHGIQHHDIHGMQHQGGHGMQHQGMHGMQHQGGHGMQHQGGHGMQHQ 179
Score = 28.3 bits (60), Expect = 2.8
Identities = 14/49 (28%), Positives = 23/49 (46%), Gaps = 2/49 (4%)
Frame = +3
Query: 276 HQGLHGTHL--RHEVEQRVHNRQGHEGVHLAAARQGHPPHHRRGAGQAH 416
HQ +HG RH ++ + + H+G+H + GH H+ G H
Sbjct: 202 HQDMHGMQHQGRHGMQHQGGHEMQHQGMHGMQHQGGHRIQHQGMHGMQH 250
Score = 27.5 bits (58), Expect = 4.8
Identities = 20/55 (36%), Positives = 27/55 (49%)
Frame = +3
Query: 276 HQGLHGTHLRHEVEQRVHNRQGHEGVHLAAARQGHPPHHRRGAGQAHRSQGRRAV 440
HQG HG ++H Q +H Q H+G H + GH H+ G H QGR +
Sbjct: 170 HQGGHG--MQH---QGMHGMQ-HQGGHGMEHQGGHGMQHQDMHGMQH--QGRHGM 216
Score = 27.1 bits (57), Expect = 6.4
Identities = 18/52 (34%), Positives = 25/52 (48%)
Frame = +3
Query: 276 HQGLHGTHLRHEVEQRVHNRQGHEGVHLAAARQGHPPHHRRGAGQAHRSQGR 431
HQG HG ++H Q +H Q H+G H + GH H+ G H+ R
Sbjct: 194 HQGGHG--MQH---QDMHGMQ-HQGRHGMQHQGGHEMQHQGMHGMQHQGGHR 239
>At1g31817.1 68414.m03907 chloroplast 30S ribosomal protein S11,
putative contains Pfam profile: PF00411: Ribosomal
protein S11
Length = 314
Score = 31.5 bits (68), Expect = 0.30
Identities = 17/44 (38%), Positives = 24/44 (54%)
Frame = +1
Query: 178 VRELVYDHGRP*LGARGHHRVPRETSGSFDIVHIKDSTGHTFAT 309
VRE++ D GR G H + P + + DI+HIK +TF T
Sbjct: 166 VREVIEDEGRRTAGIFSHFQRPNLETNA-DIIHIKMLRNNTFVT 208
>At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3
domain-containing protein similar to eukaryotic protein
synthesis initiation factor [Homo sapiens] GI:3941724;
contains Pfam profiles PF02854: MIF4G domain, PF02847:
MA3 domain
Length = 1544
Score = 28.7 bits (61), Expect = 2.1
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = +3
Query: 372 QGHPPHHRRGAGQAHRSQG 428
QGHPPH G H++QG
Sbjct: 132 QGHPPHPMHHQGMMHQAQG 150
>At1g68670.1 68414.m07846 myb family transcription factor contains
Pfam domain, PF00249: Myb-like DNA-binding domain
Length = 354
Score = 28.7 bits (61), Expect = 2.1
Identities = 20/48 (41%), Positives = 23/48 (47%)
Frame = -2
Query: 304 RRCVPWSP*CAQCRRSRMSLAGHDGAHAPQVTASRDHTQVPGLKLDEV 161
RRC WSP RR +L G+H RDH +V GL DEV
Sbjct: 217 RRC--WSP--ELHRRFLNALQQLGGSHVATPKQIRDHMKVDGLTNDEV 260
>At1g14210.1 68414.m01682 ribonuclease T2 family protein contains
similarity to RNase GI:7768564 from [Nicotiana tabacum];
contains Pfam profile PF00445: Ribonuclease T2 family
Length = 247
Score = 28.7 bits (61), Expect = 2.1
Identities = 17/60 (28%), Positives = 27/60 (45%)
Frame = +3
Query: 156 LWTSSSLSPGTCV*SREAVTWGAWAPSCPARDIRLLRHCAHQGLHGTHLRHEVEQRVHNR 335
L+ S +S C ++ WG WA CP+ + L H HGT ++ +Q + R
Sbjct: 81 LFDISKISDLVCQMEKKWTEWGVWA--CPSNETNLWEH--EWNKHGTCVQSIFDQHSYFR 136
>At2g26730.1 68415.m03206 leucine-rich repeat transmembrane protein
kinase, putative
Length = 658
Score = 27.9 bits (59), Expect = 3.7
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +2
Query: 74 HDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITG 205
H+ + +P ++N+ I+LDI++ I F NL +TG
Sbjct: 124 HNEFSGEFPTSFTQLNNLIRLDISSNNFTGSIPFSVNNLTHLTG 167
>At3g45570.1 68416.m04922 zinc finger (C3HC4-type RING finger)
family protein contains a zinc finger, C3HC4 type (RING
finger), signature, PROSITE:PS00518
Length = 312
Score = 27.5 bits (58), Expect = 4.8
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -1
Query: 461 KVYCP*PYCAATLAAMRLSRSS 396
+VYCP P C++ ++ +LS S+
Sbjct: 247 RVYCPNPLCSSLMSVTKLSNST 268
>At2g38570.1 68415.m04738 expressed protein ; expression supported
by MPSS
Length = 302
Score = 27.5 bits (58), Expect = 4.8
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +2
Query: 149 TKIMDFIKFESGNLCMITGGRNLGRVGT 232
T I+ +IK G + GGRN+G+V T
Sbjct: 196 TYILVYIKISVGYRVVTPGGRNIGKVST 223
>At5g60250.1 68418.m07552 zinc finger (C3HC4-type RING finger)
family protein contains a Prosite:PS00518 Zinc finger,
C3HC4 type (RING finger), signature and Pfam domain
PF01485: IBR domain
Length = 655
Score = 27.1 bits (57), Expect = 6.4
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -1
Query: 461 KVYCP*PYCAATLAAMRLSRSS 396
+VYCP P C+A ++ ++S S+
Sbjct: 384 RVYCPYPRCSALMSKTKISESA 405
>At3g45580.1 68416.m04923 zinc finger (C3HC4-type RING finger)
family protein contains a Prosite:PS00518 Zinc finger,
C3HC4 type (RING finger), signature
Length = 408
Score = 27.1 bits (57), Expect = 6.4
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -1
Query: 461 KVYCP*PYCAATLAAMRLSRSS 396
+VYCP P C++ ++ +LS S+
Sbjct: 257 RVYCPNPRCSSLMSVTKLSNST 278
>At3g22330.1 68416.m02820 DEAD box RNA helicase, putative similar to
RNA helicases GI:3775995, GI:3775987 from [Arabidopsis
thaliana]; contains Pfam profiles PF00270: DEAD/DEAH box
helicase, PF00271: Helicase conserved C-terminal domain
Length = 616
Score = 27.1 bits (57), Expect = 6.4
Identities = 16/61 (26%), Positives = 29/61 (47%)
Frame = -3
Query: 297 VSRGVLDVHNVEGAGCLSRDTMVPTRPKLRPPVIIHKFPDSNLMKSIIFVVAMSNWMESL 118
+ RG L++ V+ D M+ +I+ K P+ +S++F M +W+ SL
Sbjct: 241 MKRGALNLSEVQFVVLDEADQMLQVGFAEDVEIILEKLPEKR--QSMMFSATMPSWIRSL 298
Query: 117 T 115
T
Sbjct: 299 T 299
>At3g63100.1 68416.m07087 glycine-rich protein
Length = 199
Score = 26.6 bits (56), Expect = 8.5
Identities = 19/55 (34%), Positives = 20/55 (36%)
Frame = +3
Query: 267 HCAHQGLHGTHLRHEVEQRVHNRQGHEGVHLAAARQGHPPHHRRGAGQAHRSQGR 431
HC H HG H H GH+ H GH H G G HR GR
Sbjct: 70 HCDH--CHGHGYGHG-----HREHGHDRGH------GHGRGHGHGHGHGHRRHGR 111
>At3g45555.1 68416.m04920 zinc finger (C3HC4-type RING finger)
family protein low similarity to UbcM4-interacting
protein 4 [Mus musculus] GI:13991706; contains Pfam
profiles PF00097: Zinc finger C3HC4 type (RING finger),
PF01485: IBR (In Between Ring fingers) domain
Length = 213
Score = 26.6 bits (56), Expect = 8.5
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -1
Query: 461 KVYCP*PYCAATLAAMRLSRSS 396
+ YCP P C+A ++ + LS+S+
Sbjct: 101 RFYCPNPRCSALMSKIELSKST 122
>At2g40760.1 68415.m05028 rhodanese-like domain-containing protein
contains rhodanese-like domain PF00581
Length = 474
Score = 26.6 bits (56), Expect = 8.5
Identities = 31/110 (28%), Positives = 45/110 (40%), Gaps = 2/110 (1%)
Frame = -3
Query: 363 QRDVRLRALADYEHVVQPRGEGVSRGVLDVHNVEGAGCLSRDTMVPTRPKLRPPVIIHKF 184
QRDVRL L E V P E + G + AG + R KL+ ++
Sbjct: 164 QRDVRLNGLRQVETPVSPEQEAIHHGHSSSSPL-AAGEDAPFRWDHVRVKLKKEIVTLGI 222
Query: 183 PDSNLMKSIIFVVAMSNWMESLTLISGSG*RM--VRPSCVTKYGTFLGPV 40
P + ++ + V+ W E LIS + VR + T+ G F G V
Sbjct: 223 PSVSPIERVGTYVSPEEWNE---LISDPETVVIDVRNTYETRIGKFKGAV 269
>At2g28130.1 68415.m03417 expressed protein
Length = 458
Score = 26.6 bits (56), Expect = 8.5
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -3
Query: 174 NLMKSIIFVVAMSNWMESLTLI 109
NL K IF+V NW+ S TL+
Sbjct: 390 NLFKMYIFLVLAENWLFSSTLV 411
>At1g62730.1 68414.m07080 expressed protein
Length = 304
Score = 26.6 bits (56), Expect = 8.5
Identities = 14/49 (28%), Positives = 23/49 (46%)
Frame = -3
Query: 303 EGVSRGVLDVHNVEGAGCLSRDTMVPTRPKLRPPVIIHKFPDSNLMKSI 157
EG+S V ++ +V A L + P PV++H P L+ S+
Sbjct: 219 EGLSNVVFEIASVANAHLLKARELAGKVPAEAKPVLLHSVPVQVLLDSL 267
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,165,739
Number of Sequences: 28952
Number of extensions: 244208
Number of successful extensions: 782
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 782
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 801831960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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