Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0793
(674 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g46230.1 68415.m05749 expressed protein contains Pfam profil... 131 5e-31
At1g26530.1 68414.m03233 expressed protein contains Pfam profil... 73 1e-13
At2g34570.1 68415.m04247 expressed protein contains Pfam profil... 36 0.033
At1g22420.1 68414.m02803 hydroxyproline-rich glycoprotein family... 33 0.23
At4g36080.1 68417.m05136 FAT domain-containing protein / phospha... 29 2.8
At3g16620.1 68416.m02124 chloroplast outer membrane protein, put... 29 2.8
At1g72760.1 68414.m08413 protein kinase family protein contains ... 28 5.0
At1g57600.1 68414.m06536 membrane bound O-acyl transferase (MBOA... 28 5.0
At3g02960.1 68416.m00291 copper-binding protein-related low simi... 28 6.6
At1g67070.1 68414.m07627 phosphomannose isomerase, putative (DIN... 28 6.6
At3g53640.1 68416.m05925 protein kinase family protein contains ... 27 8.7
At1g17770.1 68414.m02199 SET domain-containing protein (SUVH7) c... 27 8.7
>At2g46230.1 68415.m05749 expressed protein contains Pfam profile:
PF04900 protein of unknown function, DUF652
Length = 196
Score = 131 bits (316), Expect = 5e-31
Identities = 58/84 (69%), Positives = 66/84 (78%)
Frame = +3
Query: 258 VPQTSSALFFQYNMQLGPPYHVLIDTNFINFSIKNKLDIIQNMMDCLYAKCIPYITDCVL 437
VP + LFF YN L PPY VL+DTNFINFSI+NK+D+ + M DCLYA C P ITDCV+
Sbjct: 44 VPSVPAGLFFSYNSTLVPPYRVLVDTNFINFSIQNKIDLEKGMRDCLYANCTPCITDCVM 103
Query: 438 GELEKLGRKYRVALRIIKDPRFER 509
ELEKLG+KYRVALRI KDP FER
Sbjct: 104 AELEKLGQKYRVALRIAKDPHFER 127
Score = 77.4 bits (182), Expect = 8e-15
Identities = 33/44 (75%), Positives = 37/44 (84%)
Frame = +2
Query: 494 PTI*EIACLHKGTYADDCLVQRVTQHKCYIVATNDKDLKRRIRK 625
P + C+HKGTYADDCLV RVTQHKC+IVAT D+DLKRRIRK
Sbjct: 123 PHFERLPCIHKGTYADDCLVDRVTQHKCFIVATCDRDLKRRIRK 166
Score = 32.3 bits (70), Expect = 0.30
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = +1
Query: 622 KIPGVPIMYVAEHKYTI 672
KIPGVPIMYV KY+I
Sbjct: 166 KIPGVPIMYVTNRKYSI 182
>At1g26530.1 68414.m03233 expressed protein contains Pfam profile:
PF04900 protein of unknown function, DUF652; expression
supported by MPSS
Length = 189
Score = 73.3 bits (172), Expect = 1e-13
Identities = 34/62 (54%), Positives = 44/62 (70%)
Frame = +2
Query: 440 RIGKTWKKV*SSFKDYQGPTI*EIACLHKGTYADDCLVQRVTQHKCYIVATNDKDLKRRI 619
++ K +K + + + P + C+ KGTYADDCLV RVTQHKC+IVAT D+DLKRRI
Sbjct: 79 KLEKLGQKYRVALRIAKDPRFERLPCVLKGTYADDCLVDRVTQHKCFIVATCDRDLKRRI 138
Query: 620 RK 625
RK
Sbjct: 139 RK 140
Score = 70.9 bits (166), Expect = 7e-13
Identities = 43/84 (51%), Positives = 48/84 (57%)
Frame = +3
Query: 258 VPQTSSALFFQYNMQLGPPYHVLIDTNFINFSIKNKLDIIQNMMDCLYAKCIPYITDCVL 437
VP S LFF +N L PPY VL+DTNFINFSI+NKL+
Sbjct: 44 VPSVPSGLFFSHNSSLVPPYRVLVDTNFINFSIQNKLE---------------------- 81
Query: 438 GELEKLGRKYRVALRIIKDPRFER 509
KLG+KYRVALRI KDPRFER
Sbjct: 82 ----KLGQKYRVALRIAKDPRFER 101
Score = 33.1 bits (72), Expect = 0.17
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = +1
Query: 622 KIPGVPIMYVAEHKYTI 672
KIPGVPIMYV + KY+I
Sbjct: 140 KIPGVPIMYVTQRKYSI 156
>At2g34570.1 68415.m04247 expressed protein contains Pfam profile:
PF04900 protein of unknown function, DUF652
Length = 281
Score = 35.5 bits (78), Expect = 0.033
Identities = 20/67 (29%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +3
Query: 282 FFQYNMQLGPPYHVLIDTNFINFSIKNKLDIIQNMMDCLYAKCIP-YITDCVLGELEKLG 458
FF PY VL D F++ + N++ + L + + T CV+ ELEKLG
Sbjct: 15 FFTVCYGFRQPYKVLCDGTFVHHLVTNEITPADTAVSELLGGPVKLFTTRCVIAELEKLG 74
Query: 459 RKYRVAL 479
+ + +L
Sbjct: 75 KDFAESL 81
>At1g22420.1 68414.m02803 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 480
Score = 32.7 bits (71), Expect = 0.23
Identities = 17/46 (36%), Positives = 30/46 (65%), Gaps = 1/46 (2%)
Frame = +1
Query: 166 INPSDSRIKNSERSEPKKKKPVDPHVIKIRKSH-KQVPHYSSNTTC 300
++P+ + +SERS+ K K+PV ++KI K H K++ H + +TC
Sbjct: 19 LDPNQTNNVSSERSKLKAKEPV---ILKIHKHHFKEMVHRLTGSTC 61
>At4g36080.1 68417.m05136 FAT domain-containing protein /
phosphatidylinositol 3- and 4-kinase family protein
contains Pfam profiles PF00454: Phosphatidylinositol 3-
and 4-kinase, PF02259: FAT domain, PF02260: FATC domain
Length = 3839
Score = 29.1 bits (62), Expect = 2.8
Identities = 18/65 (27%), Positives = 29/65 (44%)
Frame = +3
Query: 258 VPQTSSALFFQYNMQLGPPYHVLIDTNFINFSIKNKLDIIQNMMDCLYAKCIPYITDCVL 437
+ T F +Q Y D INF + +KLD+++N D K I ++ C+
Sbjct: 582 IDNTQLVQIFATLLQAPKVYKPFADV-LINFLVSSKLDVLKN-PDSAATKLILHLFRCLF 639
Query: 438 GELEK 452
G + K
Sbjct: 640 GAVSK 644
>At3g16620.1 68416.m02124 chloroplast outer membrane protein,
putative similar to chloroplast protein import component
Toc159 [Pisum sativum] GI:8489806, chloroplast outer
envelope protein 86 [Pisum sativum] GI:599958,
GTP-binding protein [Pisum sativum] GI:576509
Length = 1089
Score = 29.1 bits (62), Expect = 2.8
Identities = 15/37 (40%), Positives = 24/37 (64%)
Frame = +1
Query: 103 MGKQRKTRKIVEKRFAKMKKMINPSDSRIKNSERSEP 213
M ++RK RK+++K A++K M N ++ ERSEP
Sbjct: 776 MKEERKRRKLLKKFAAEIKDMPNGYSENVE-EERSEP 811
>At1g72760.1 68414.m08413 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 697
Score = 28.3 bits (60), Expect = 5.0
Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 2/46 (4%)
Frame = +3
Query: 57 IEVKVFGIQIFINHYNGQ--TTKNEENSREKICKNEKNDQPQRLSD 188
+E +V +++ + +N TT +ENS+E +EK + P+ LSD
Sbjct: 274 LEAEVRRLRLELKQFNKDKDTTNQKENSQETPWSDEKIELPRALSD 319
>At1g57600.1 68414.m06536 membrane bound O-acyl transferase (MBOAT)
family protein low similarity to skinny hedgehog
[Drosophila melanogaster] GI:15420842; contains Pfam
profile PF03062: MBOAT family
Length = 533
Score = 28.3 bits (60), Expect = 5.0
Identities = 9/26 (34%), Positives = 19/26 (73%)
Frame = -2
Query: 628 VFSNSSFQIFVIGSYNITFMLCNSLY 551
VF+ +S+ +++ ++NI F+ CN +Y
Sbjct: 137 VFARTSYFPYMLWTFNIFFLFCNRIY 162
>At3g02960.1 68416.m00291 copper-binding protein-related low
similarity to copper homeostasis factor gi:3168840 from
Arabidopsis thaliana; contains Pfam profile PF00403:
Heavy-metal-associated domain
Length = 246
Score = 27.9 bits (59), Expect = 6.6
Identities = 15/53 (28%), Positives = 30/53 (56%), Gaps = 3/53 (5%)
Frame = +1
Query: 94 IIIMGKQRKTRKI---VEKRFAKMKKMINPSDSRIKNSERSEPKKKKPVDPHV 243
+++ GK KI V+K+F++ +MI+P + ++ EP++KK P +
Sbjct: 75 VVVSGKFDDPLKILRRVQKKFSRNAEMISPKHN--PKQDQKEPQQKKESAPEI 125
>At1g67070.1 68414.m07627 phosphomannose isomerase, putative (DIN9)
contains Pfam profile: PF01238 phosphomannose isomerase
type I ;similar to phosphomannose isomerase GI:10834550
from [Arabidopsis thaliana]; identical to cDNA
phosphomannose isomerase (din9) partial cds GI:10834549
Length = 441
Score = 27.9 bits (59), Expect = 6.6
Identities = 13/21 (61%), Positives = 17/21 (80%)
Frame = -1
Query: 515 MLSLKSWVLDNP*SYSILSSK 453
M++LKSWVLDNP ++L SK
Sbjct: 89 MVTLKSWVLDNP---NLLGSK 106
>At3g53640.1 68416.m05925 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 642
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +1
Query: 127 KIVEKRFAKMKKMINPSDSRIKNSERSEPKKKKPVDPHVIKIRKSHKQVPHYSSN 291
KIVE K ++ +PSD +K+S+R + K H + + K V + N
Sbjct: 5 KIVESSHRKHRRSYSPSDEVVKSSKRHKHHHHKHRHSHHLDEDGNEKNVYPFLGN 59
>At1g17770.1 68414.m02199 SET domain-containing protein (SUVH7)
contains Pfam profiles: PF05033: Pre-SET motif, PF00856
SET domain; identical to cDNA SUVH7 (SUVH7) GI:13517754
Length = 693
Score = 27.5 bits (58), Expect = 8.7
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1
Query: 190 KNSERSEPKKKKPVDPHVIKI 252
KNS +EPKK K DP+ +K+
Sbjct: 139 KNSTPTEPKKPKVYDPNSLKV 159
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,236,555
Number of Sequences: 28952
Number of extensions: 288668
Number of successful extensions: 800
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 774
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 798
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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