Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0788
(781 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 153 2e-38
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 153 2e-38
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 153 2e-38
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 79 7e-16
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 75 9e-15
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 51 2e-07
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 30 0.32
SPAC23D3.08 |usp108||U1 snRNP-associated protein Usp108|Schizosa... 28 1.3
SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces po... 27 3.0
SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 27 3.0
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.0
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 5.3
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 25 9.2
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 9.2
SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding... 25 9.2
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 153 bits (372), Expect = 2e-38
Identities = 67/87 (77%), Positives = 76/87 (87%)
Frame = +3
Query: 504 PRNITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFT 683
P +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FT
Sbjct: 280 PAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFT 339
Query: 684 AQVIVLNHPGQISNGYTPVLDCHTAHM 764
AQVI+LNHPGQIS GY+PVLDCHTAH+
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHI 366
Score = 150 bits (363), Expect = 3e-37
Identities = 66/85 (77%), Positives = 74/85 (87%)
Frame = +1
Query: 256 DNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGG 435
DNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGG
Sbjct: 197 DNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGG 256
Query: 436 IGTVPVGRVETGVLKPGTIVVFAPA 510
IGTVPVGRVETGV+KPG IV FAPA
Sbjct: 257 IGTVPVGRVETGVIKPGMIVTFAPA 281
Score = 124 bits (299), Expect = 1e-29
Identities = 59/83 (71%), Positives = 71/83 (85%)
Frame = +2
Query: 2 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 181
LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE
Sbjct: 114 LIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKE 171
Query: 182 VSSYIKKIGYNPAAVAFVPISGW 250
S++IKK+G+NP V FVP+SG+
Sbjct: 172 TSNFIKKVGFNPKTVPFVPVSGF 194
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 153 bits (372), Expect = 2e-38
Identities = 67/87 (77%), Positives = 76/87 (87%)
Frame = +3
Query: 504 PRNITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFT 683
P +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FT
Sbjct: 280 PAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFT 339
Query: 684 AQVIVLNHPGQISNGYTPVLDCHTAHM 764
AQVI+LNHPGQIS GY+PVLDCHTAH+
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHI 366
Score = 150 bits (363), Expect = 3e-37
Identities = 66/85 (77%), Positives = 74/85 (87%)
Frame = +1
Query: 256 DNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGG 435
DNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGG
Sbjct: 197 DNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGG 256
Query: 436 IGTVPVGRVETGVLKPGTIVVFAPA 510
IGTVPVGRVETGV+KPG IV FAPA
Sbjct: 257 IGTVPVGRVETGVIKPGMIVTFAPA 281
Score = 124 bits (299), Expect = 1e-29
Identities = 59/83 (71%), Positives = 71/83 (85%)
Frame = +2
Query: 2 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 181
LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE
Sbjct: 114 LIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKE 171
Query: 182 VSSYIKKIGYNPAAVAFVPISGW 250
S++IKK+G+NP V FVP+SG+
Sbjct: 172 TSNFIKKVGFNPKTVPFVPVSGF 194
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 153 bits (372), Expect = 2e-38
Identities = 67/87 (77%), Positives = 76/87 (87%)
Frame = +3
Query: 504 PRNITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFT 683
P +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FT
Sbjct: 280 PAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFT 339
Query: 684 AQVIVLNHPGQISNGYTPVLDCHTAHM 764
AQVI+LNHPGQIS GY+PVLDCHTAH+
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHI 366
Score = 150 bits (363), Expect = 3e-37
Identities = 66/85 (77%), Positives = 74/85 (87%)
Frame = +1
Query: 256 DNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGG 435
DNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGG
Sbjct: 197 DNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGG 256
Query: 436 IGTVPVGRVETGVLKPGTIVVFAPA 510
IGTVPVGRVETGV+KPG IV FAPA
Sbjct: 257 IGTVPVGRVETGVIKPGMIVTFAPA 281
Score = 124 bits (299), Expect = 1e-29
Identities = 59/83 (71%), Positives = 71/83 (85%)
Frame = +2
Query: 2 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 181
LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE
Sbjct: 114 LIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKE 171
Query: 182 VSSYIKKIGYNPAAVAFVPISGW 250
S++IKK+G+NP V FVP+SG+
Sbjct: 172 TSNFIKKVGFNPKTVPFVPVSGF 194
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 79.0 bits (186), Expect = 7e-16
Identities = 36/85 (42%), Positives = 58/85 (68%), Gaps = 2/85 (2%)
Frame = +2
Query: 2 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 181
L+++A GEFEAG + GQTREHA+LA T G+ L+V +NKMD +SE R++E +
Sbjct: 345 LVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDK 404
Query: 182 VSSYIKKI-GYNPAA-VAFVPISGW 250
+S +++++ GYN V ++P+S +
Sbjct: 405 LSMFLRRVAGYNSKTDVKYMPVSAY 429
Score = 44.8 bits (101), Expect = 1e-05
Identities = 27/86 (31%), Positives = 44/86 (51%), Gaps = 1/86 (1%)
Frame = +3
Query: 504 PRNITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADF 680
P N T EV ++ + E + ++ GD V V+ +++ GYV +KN P F
Sbjct: 503 PINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRF 560
Query: 681 TAQVIVLNHPGQISNGYTPVLDCHTA 758
AQ+ +L P ++ GY+ V+ HTA
Sbjct: 561 IAQIAILELPSILTTGYSCVMHIHTA 586
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 75.4 bits (177), Expect = 9e-15
Identities = 39/82 (47%), Positives = 53/82 (64%), Gaps = 1/82 (1%)
Frame = +2
Query: 2 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 181
L+V + FE G +NGQTREHA L LG+ +++V VNK+D +SE RF+EIK
Sbjct: 284 LVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQEIKNI 341
Query: 182 VSSY-IKKIGYNPAAVAFVPIS 244
VS + IK +G+ + V FVPIS
Sbjct: 342 VSDFLIKMVGFKTSNVHFVPIS 363
Score = 45.2 bits (102), Expect = 1e-05
Identities = 23/46 (50%), Positives = 29/46 (63%)
Frame = +1
Query: 334 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETG 471
G L+ ALD ++PP +P KPLRL + DVY+ TV GRVE G
Sbjct: 384 GPTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAG 428
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 51.2 bits (117), Expect = 2e-07
Identities = 27/65 (41%), Positives = 39/65 (60%), Gaps = 1/65 (1%)
Frame = +1
Query: 343 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPATSL 519
L+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G + S
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSH 293
Query: 520 LKSSL 534
LK+++
Sbjct: 294 LKTTV 298
Score = 45.2 bits (102), Expect = 1e-05
Identities = 22/46 (47%), Positives = 31/46 (67%)
Frame = +2
Query: 56 QTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 193
QTREH LLA +GVKQ++V +NK+D EP E E+++ +S Y
Sbjct: 156 QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEMEMRELLSEY 201
Score = 34.7 bits (76), Expect = 0.015
Identities = 14/42 (33%), Positives = 26/42 (61%)
Frame = +3
Query: 510 NITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 635
++ T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 293 HLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.3 bits (65), Expect = 0.32
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +1
Query: 283 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 462
+P K V K GK + L E + I PP + PLR L D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 463 ETGVLKPG 486
G +K G
Sbjct: 266 MEGFIKKG 273
>SPAC23D3.08 |usp108||U1 snRNP-associated protein
Usp108|Schizosaccharomyces pombe|chr 1|||Manual
Length = 382
Score = 28.3 bits (60), Expect = 1.3
Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 3/76 (3%)
Frame = -3
Query: 623 TQFLDGHVLYVET---YIVSRYSFLESFVVHLHRLDFSSDVAGAKTTMVPGFNTPVSTLP 453
T +D V+ +ET YI+SR SF+ V + D + + G+ +T + P
Sbjct: 162 TNEIDASVV-IETLLKYILSRISFIPRVSVVPYIPDAAYGIYGSPSTFTQFNHMSSMVTP 220
Query: 452 TGTVPIPPILYTSCRG 405
IPPI TS G
Sbjct: 221 ENPYGIPPIALTSAGG 236
>SPBC18H10.20c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 361
Score = 27.1 bits (57), Expect = 3.0
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = -1
Query: 583 TLSPGTASWRASWCISTDLTSVVMLRGQRQQWYLVST 473
T PGT W SW L + V R + ++YL +T
Sbjct: 101 TYRPGTQHWPFSWLFPGSLPASVSNRYIKLEYYLEAT 137
>SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1208
Score = 27.1 bits (57), Expect = 3.0
Identities = 19/52 (36%), Positives = 26/52 (50%)
Frame = -3
Query: 353 ASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSRAIQKWARKRQQLGCSQSS 198
A +RH S L H LN+GI E +N+ A++K R +GC SS
Sbjct: 774 AQIRHLESDKMELDKLVHHLNRGI--EEANIEENAVKK--RLCLLMGCDYSS 821
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -3
Query: 620 QFLDGHVLYVETYIVSRYSFLESFV 546
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 5.3
Identities = 22/72 (30%), Positives = 25/72 (34%), Gaps = 5/72 (6%)
Frame = -3
Query: 485 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 321
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Query: 320 SLRSTCHPLNQG 285
P G
Sbjct: 374 PADGVPKPTPDG 385
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 25.4 bits (53), Expect = 9.2
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +3
Query: 147 TVSPDLRKSRRKYPHTSRRLATTQLLSLSC 236
+ +PD+ ++PHT +R +T + L + C
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRC 174
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 9.2
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 476 NTPVSTLPTGTVPIPPIL 423
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding
protein Ssb2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 279
Score = 25.4 bits (53), Expect = 9.2
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -3
Query: 569 YSFLESFVVHLHRLDFSSDVAGAKTTMVPGFNT 471
+ FLE+ VHLH ++ V GA PG+ T
Sbjct: 158 FHFLEAIAVHLHFTQKANAVNGAN---APGYGT 187
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,686,436
Number of Sequences: 5004
Number of extensions: 84418
Number of successful extensions: 266
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 239
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 261
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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