Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0780
         (779 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc...   118   1e-27
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac...    58   1e-09
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa...    28   1.7  

>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 426

 Score =  118 bits (283), Expect = 1e-27
 Identities = 52/90 (57%), Positives = 71/90 (78%)
 Frame = +1

Query: 1   DGEFELMSYRLNTHVKPLIWIESVIERHAHSRVEYMIKAKSQFKRRSTANNVEIIIPVPA 180
           DGEF+LMSYR++++V+PLIW+E     H+ SR+E+M+KAK+QFK+R  ANNV+IIIPVP 
Sbjct: 259 DGEFDLMSYRMSSNVRPLIWVECESIVHSGSRIEFMVKAKAQFKKRCIANNVQIIIPVPE 318

Query: 181 DADSPKFKTTIGSVKYTPEQNAITCQSNHF 270
           DADSP+F+T+ G V+Y PEQ A+      F
Sbjct: 319 DADSPRFQTSNGHVQYAPEQAAMVWNIKKF 348



 Score =  107 bits (257), Expect = 2e-24
 Identities = 53/82 (64%), Positives = 63/82 (76%), Gaps = 4/82 (4%)
 Frame = +3

Query: 255 SIKSFPGGKEYLMRAHFGLPSVECEE--VDGKPPIQVKFEIPYFTTSGIQVRYLKIIE-K 425
           +IK F GGKE+ MRA  GLPSV+ E+  V  K P+Q+KF IPYFTTSGIQVRYLKI E K
Sbjct: 344 NIKKFAGGKEFFMRAEMGLPSVKNEDIQVQKKRPVQLKFAIPYFTTSGIQVRYLKITEPK 403

Query: 426 SGYQALPWVRYITQNG-DYQLR 488
             Y A+PWVRY+TQNG +Y +R
Sbjct: 404 LNYHAMPWVRYVTQNGTEYSIR 425


>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 446

 Score = 58.0 bits (134), Expect = 1e-09
 Identities = 29/79 (36%), Positives = 46/79 (58%), Gaps = 2/79 (2%)
 Frame = +3

Query: 258 IKSFPGGKEYLMRAHFGLPSVECEEVDGKPPIQVKFEIPYFTTSGIQVRYLKIIEKSG-- 431
           I  F G  E +  A   L +   +++  KPPI + F I  FT+SG+ V+YL++ E S   
Sbjct: 367 IPRFLGETELIFYAEVELSNTTNQQIWAKPPISLDFNILMFTSSGLHVQYLRVSEPSNSK 426

Query: 432 YQALPWVRYITQNGDYQLR 488
           Y+++ WVRY T+ G  ++R
Sbjct: 427 YKSIKWVRYSTRAGTCEIR 445



 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 23/90 (25%), Positives = 44/90 (48%)
 Frame = +1

Query: 1   DGEFELMSYRLNTHVKPLIWIESVIERHAHSRVEYMIKAKSQFKRRSTANNVEIIIPVPA 180
           DGE ELMSYR + ++     I  ++E+ +  ++ Y I  ++ +  +  ++++   IPVP 
Sbjct: 282 DGEVELMSYRSHENINIPFRIVPIVEQLSKQKIIYRISIRADYPHK-LSSSLNFRIPVPT 340

Query: 181 DADSPKFKTTIGSVKYTPEQNAITCQSNHF 270
           +      +   G   Y P +N I  +   F
Sbjct: 341 NVVKANPRVNRGKAGYEPSENIINWKIPRF 370


>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1628

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 12/40 (30%), Positives = 25/40 (62%), Gaps = 1/40 (2%)
 Frame = +2

Query: 191  HQNSRQQLVAL-NIHQNKMRSHVNQIISRRQGVLNESSFW 307
            H++ RQ+++ L N  QN+++ H+N + S R+ +  +   W
Sbjct: 1019 HRHDRQKIMQLSNSLQNELKEHINSLESVRKVLQGDCVKW 1058


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,914,521
Number of Sequences: 5004
Number of extensions: 56446
Number of successful extensions: 115
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)