Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0780
(779 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g60780.1 68414.m06842 clathrin adaptor complexes medium subun... 126 2e-29
At1g10730.1 68414.m01223 clathrin adaptor complexes medium subun... 113 9e-26
At5g46630.1 68418.m05741 clathrin adaptor complexes medium subun... 81 6e-16
At5g46630.2 68418.m05740 clathrin adaptor complexes medium subun... 56 3e-08
At4g24550.2 68417.m03519 clathrin adaptor complexes medium subun... 50 1e-06
At4g24550.1 68417.m03518 clathrin adaptor complexes medium subun... 47 1e-05
At5g38590.1 68418.m04667 F-box family protein contains F-box dom... 31 0.65
At5g64220.1 68418.m08067 calmodulin-binding protein similar to a... 31 1.1
At1g05670.1 68414.m00588 UDP-glucoronosyl/UDP-glucosyl transfera... 28 8.0
>At1g60780.1 68414.m06842 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 126 bits (304), Expect = 2e-29
Identities = 59/79 (74%), Positives = 66/79 (83%), Gaps = 2/79 (2%)
Frame = +3
Query: 258 IKSFPGGKEYLMRAHFGLPSVECEEV--DGKPPIQVKFEIPYFTTSGIQVRYLKIIEKSG 431
IKSFPG KEY++RA F LPS+ EE + K PI+VKFEIPYFT SGIQVRYLKIIEKSG
Sbjct: 348 IKSFPGNKEYMLRAEFHLPSITAEEATPERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSG 407
Query: 432 YQALPWVRYITQNGDYQLR 488
YQALPWVRYIT G+Y+LR
Sbjct: 408 YQALPWVRYITMAGEYELR 426
Score = 118 bits (283), Expect = 6e-27
Identities = 52/90 (57%), Positives = 69/90 (76%)
Frame = +1
Query: 1 DGEFELMSYRLNTHVKPLIWIESVIERHAHSRVEYMIKAKSQFKRRSTANNVEIIIPVPA 180
DG F+LM+YRL+T VKPLIW+E+ IE H+ SRVE +IKA+SQFK RSTA NVEI +PVP
Sbjct: 262 DGAFDLMTYRLSTQVKPLIWVEAQIESHSRSRVEMLIKARSQFKERSTATNVEIELPVPT 321
Query: 181 DADSPKFKTTIGSVKYTPEQNAITCQSNHF 270
DA +P +T++GS Y PE++A+ + F
Sbjct: 322 DASNPTVRTSLGSASYAPEKDALVWKIKSF 351
>At1g10730.1 68414.m01223 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 113 bits (273), Expect = 9e-26
Identities = 50/90 (55%), Positives = 70/90 (77%)
Frame = +1
Query: 1 DGEFELMSYRLNTHVKPLIWIESVIERHAHSRVEYMIKAKSQFKRRSTANNVEIIIPVPA 180
DG F+LM+YRL+T VKPLIW+E+ IERH+ SRVE ++KA+SQFK RS A +VEI +PVP
Sbjct: 262 DGSFDLMTYRLSTQVKPLIWVEAHIERHSRSRVEMLVKARSQFKDRSYATSVEIELPVPT 321
Query: 181 DADSPKFKTTIGSVKYTPEQNAITCQSNHF 270
DA +P +T++GS Y PE++A+ + +F
Sbjct: 322 DAYNPDVRTSLGSAAYAPEKDALVWKIQYF 351
Score = 104 bits (250), Expect = 6e-23
Identities = 51/79 (64%), Positives = 60/79 (75%), Gaps = 2/79 (2%)
Frame = +3
Query: 258 IKSFPGGKEYLMRAHFGLPSVECEEV--DGKPPIQVKFEIPYFTTSGIQVRYLKIIEKSG 431
I+ F G KE+ ++A F LPS+ EE + K PI+VKFEIP F SGIQVRYLKIIEKSG
Sbjct: 348 IQYFYGNKEHTLKADFHLPSIAAEEATPERKAPIRVKFEIPKFIVSGIQVRYLKIIEKSG 407
Query: 432 YQALPWVRYITQNGDYQLR 488
YQA PWVRYIT G+Y+LR
Sbjct: 408 YQAHPWVRYITMAGEYELR 426
>At5g46630.1 68418.m05741 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 438
Score = 81.4 bits (192), Expect = 6e-16
Identities = 38/83 (45%), Positives = 54/83 (65%), Gaps = 1/83 (1%)
Frame = +3
Query: 243 CDHMSIKSFPGGKEYLMRAHFGLPSVECEEVDG-KPPIQVKFEIPYFTTSGIQVRYLKII 419
C I+ FPG E + A L S E+ +PPIQ++F++P FT SG++VR+LK+
Sbjct: 355 CLVWKIRKFPGQTESTLSAEIELISTMGEKKSWTRPPIQMEFQVPMFTASGLRVRFLKVW 414
Query: 420 EKSGYQALPWVRYITQNGDYQLR 488
EKSGY + WVRYIT+ G Y++R
Sbjct: 415 EKSGYNTVEWVRYITKAGSYEIR 437
Score = 55.6 bits (128), Expect = 3e-08
Identities = 30/90 (33%), Positives = 42/90 (46%)
Frame = +1
Query: 1 DGEFELMSYRLNTHVKPLIWIESVIERHAHSRVEYMIKAKSQFKRRSTANNVEIIIPVPA 180
DGEFELM YR+ V + I+ +R+E +K KS F + A V + IPVP
Sbjct: 274 DGEFELMKYRITEGVNLPFRVLPTIKELGRTRMEVNVKVKSVFGAKMFALGVVVKIPVPK 333
Query: 181 DADSPKFKTTIGSVKYTPEQNAITCQSNHF 270
F+ T G KY P + + + F
Sbjct: 334 QTAKTNFQVTTGRAKYNPSIDCLVWKIRKF 363
>At5g46630.2 68418.m05740 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 441
Score = 55.6 bits (128), Expect = 3e-08
Identities = 30/90 (33%), Positives = 42/90 (46%)
Frame = +1
Query: 1 DGEFELMSYRLNTHVKPLIWIESVIERHAHSRVEYMIKAKSQFKRRSTANNVEIIIPVPA 180
DGEFELM YR+ V + I+ +R+E +K KS F + A V + IPVP
Sbjct: 274 DGEFELMKYRITEGVNLPFRVLPTIKELGRTRMEVNVKVKSVFGAKMFALGVVVKIPVPK 333
Query: 181 DADSPKFKTTIGSVKYTPEQNAITCQSNHF 270
F+ T G KY P + + + F
Sbjct: 334 QTAKTNFQVTTGRAKYNPSIDCLVWKIRKF 363
Score = 48.8 bits (111), Expect = 4e-06
Identities = 24/59 (40%), Positives = 36/59 (61%), Gaps = 1/59 (1%)
Frame = +3
Query: 243 CDHMSIKSFPGGKEYLMRAHFGLPSVECEEVDG-KPPIQVKFEIPYFTTSGIQVRYLKI 416
C I+ FPG E + A L S E+ +PPIQ++F++P FT SG++VR+LK+
Sbjct: 355 CLVWKIRKFPGQTESTLSAEIELISTMGEKKSWTRPPIQMEFQVPMFTASGLRVRFLKV 413
>At4g24550.2 68417.m03519 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 451
Score = 50.4 bits (115), Expect = 1e-06
Identities = 27/80 (33%), Positives = 41/80 (51%), Gaps = 2/80 (2%)
Frame = +3
Query: 255 SIKSFPGGKEYLMRAHFGLPSVECEEVDGKP-PIQVKFEIPYFTTSGIQVRYLKIIEKSG 431
++K GG E+ +RA + + P+ + F IP + S +QV+YL+I +KS
Sbjct: 371 NLKKIVGGGEHTLRAKLTFSQEFHGNITKEAGPVSMTFTIPMYNVSKLQVKYLQIAKKSS 430
Query: 432 -YQALPWVRYITQNGDYQLR 488
Y WVRY+TQ Y R
Sbjct: 431 SYNPYRWVRYVTQANSYVAR 450
Score = 47.2 bits (107), Expect = 1e-05
Identities = 21/73 (28%), Positives = 38/73 (52%)
Frame = +1
Query: 1 DGEFELMSYRLNTHVKPLIWIESVIERHAHSRVEYMIKAKSQFKRRSTANNVEIIIPVPA 180
DGEF +M+YR+ KP + ++IE + E +IK +++F AN + + +P+P
Sbjct: 282 DGEFPVMNYRMTQEFKPPFHVNTLIEEAGRLKAEVIIKIRAEFPSDIIANTITVQMPLPN 341
Query: 181 DADSPKFKTTIGS 219
F+ G+
Sbjct: 342 YTSRASFELEPGA 354
>At4g24550.1 68417.m03518 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 380
Score = 47.2 bits (107), Expect = 1e-05
Identities = 21/73 (28%), Positives = 38/73 (52%)
Frame = +1
Query: 1 DGEFELMSYRLNTHVKPLIWIESVIERHAHSRVEYMIKAKSQFKRRSTANNVEIIIPVPA 180
DGEF +M+YR+ KP + ++IE + E +IK +++F AN + + +P+P
Sbjct: 282 DGEFPVMNYRMTQEFKPPFHVNTLIEEAGRLKAEVIIKIRAEFPSDIIANTITVQMPLPN 341
Query: 181 DADSPKFKTTIGS 219
F+ G+
Sbjct: 342 YTSRASFELEPGA 354
>At5g38590.1 68418.m04667 F-box family protein contains F-box domain
Pfam:PF00646
Length = 289
Score = 31.5 bits (68), Expect = 0.65
Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +3
Query: 243 CDHMS-IKSFPGGKEYLMRAHFGLPSVECEEVDGKPPIQVKFEIPYFTTSGIQ 398
CDH+S IK+ P +E + A LP E + + + ++P+ +T+G Q
Sbjct: 234 CDHLSLIKNMPKLREAYVDAKCFLPKSVIESITSVKHLTICSKVPFLSTAGCQ 286
>At5g64220.1 68418.m08067 calmodulin-binding protein similar to
anther ethylene-upregulated calmodulin-binding protein
ER1 GI:11612392 from[Nicotiana tabacum]
Length = 1050
Score = 30.7 bits (66), Expect = 1.1
Identities = 16/47 (34%), Positives = 22/47 (46%)
Frame = +1
Query: 139 STANNVEIIIPVPADADSPKFKTTIGSVKYTPEQNAITCQSNHFQEA 279
STA I+ P+ DADS + S++ PE + Q H Q A
Sbjct: 163 STATRSSILSPLCEDADSGDSRQASSSLQQNPEPQTVVPQIMHHQNA 209
>At1g05670.1 68414.m00588 UDP-glucoronosyl/UDP-glucosyl transferase
family protein similar to UDP-glucose:salicylic acid
glucosyltransferase [Nicotiana tabacum] GI:7385017;
contains Pfam profiles PF00201: UDP-glucoronosyl and
UDP-glucosyl transferase, PF01535: PPR repeat
Length = 1184
Score = 27.9 bits (59), Expect = 8.0
Identities = 13/45 (28%), Positives = 24/45 (53%)
Frame = +1
Query: 109 IKAKSQFKRRSTANNVEIIIPVPADADSPKFKTTIGSVKYTPEQN 243
I SQF +R + +++I + + +D SP +KT ++ P N
Sbjct: 18 ITPMSQFCKRLASKSLKITLVLVSDKPSPPYKTEHDTITVVPISN 62
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,377,442
Number of Sequences: 28952
Number of extensions: 273696
Number of successful extensions: 557
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 540
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 555
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1746037600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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