Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0750
(789 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 116 5e-27
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 116 5e-27
SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 27 3.1
SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces ... 27 4.0
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 5.4
SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc... 26 5.4
SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizos... 26 7.1
SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces pom... 26 7.1
SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces... 25 9.4
>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 116 bits (278), Expect = 5e-27
Identities = 54/91 (59%), Positives = 56/91 (61%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
QTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH
Sbjct: 61 QTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEK 120
Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 527
ARGH IE P P
Sbjct: 121 RYAIASAVAASGVPSLLLARGHRIEEIPEVP 151
Score = 114 bits (274), Expect = 2e-26
Identities = 51/93 (54%), Positives = 70/93 (75%)
Frame = +2
Query: 509 KIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRK 688
+IPE+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RNRR +QR+
Sbjct: 146 EIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNRRHVQRR 205
Query: 689 GPLIIFNKDQGLTRAFPQHPRCGAPNVNKLNLL 787
GPL++FN+D G+ +AF P NV +LNLL
Sbjct: 206 GPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNLL 238
Score = 77.4 bits (182), Expect = 2e-15
Identities = 35/62 (56%), Positives = 46/62 (74%)
Frame = +1
Query: 73 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 252
++ ARP VS+Y+ K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++AG
Sbjct: 1 MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59
Query: 253 HK 258
H+
Sbjct: 60 HQ 61
>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 116 bits (278), Expect = 5e-27
Identities = 54/91 (59%), Positives = 56/91 (61%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
QTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH
Sbjct: 61 QTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEK 120
Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 527
ARGH IE P P
Sbjct: 121 RYAISSAVAASGVPSLLLARGHRIEEIPEVP 151
Score = 113 bits (273), Expect = 2e-26
Identities = 51/93 (54%), Positives = 70/93 (75%)
Frame = +2
Query: 509 KIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRK 688
+IPE+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RNRR +QR+
Sbjct: 146 EIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNRRHVQRR 205
Query: 689 GPLIIFNKDQGLTRAFPQHPRCGAPNVNKLNLL 787
GPL++FN+D G+ +AF P NV +LNLL
Sbjct: 206 GPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNLL 238
Score = 78.2 bits (184), Expect = 1e-15
Identities = 36/62 (58%), Positives = 46/62 (74%)
Frame = +1
Query: 73 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 252
++ ARP VS+YS K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++AG
Sbjct: 1 MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59
Query: 253 HK 258
H+
Sbjct: 60 HQ 61
>SPBC23G7.08c |rga7||GTPase activating protein
Rga7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 27.1 bits (57), Expect = 3.1
Identities = 25/78 (32%), Positives = 33/78 (42%), Gaps = 4/78 (5%)
Frame = +1
Query: 76 SVARPLVSVYSEKS-ETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS---K 243
SVA P S + T +A PL + ++P ND + S S +S P S
Sbjct: 381 SVASPAFPNSSTSNPSTAPASASPLA----STLKPSTANDTNGSSSSSSSNPRTSSPLAS 436
Query: 244 EAGHKPVPNHGVPDVLLP 297
A +KP P VLLP
Sbjct: 437 NAENKPPVAQQSPPVLLP 454
>SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 345
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/41 (29%), Positives = 18/41 (43%)
Frame = -2
Query: 518 RESFNNVSSSLNERWDASSSNGCCQGRSPLSEVDATVPAPP 396
R+S V+ N W + + GC G L + A + PP
Sbjct: 178 RDSTRQVTIVPNRHWGGNGAIGCGVGHGVLHRLPAPLSGPP 218
>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 606
Score = 26.2 bits (55), Expect = 5.4
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = -2
Query: 134 APCTVSLFSEYTDTKGRATDRLISLL 57
A C V ++SEYT G ++ L++L+
Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515
>SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1063
Score = 26.2 bits (55), Expect = 5.4
Identities = 23/68 (33%), Positives = 37/68 (54%), Gaps = 6/68 (8%)
Frame = +2
Query: 593 LKAWSDILKVYKSQRLRAGKGK---MRNRRRIQRKGPLIIFNKDQGLTRAFPQHPRC--- 754
+K W D+L++ +SQRL + K M R+ +Q +I+ ++ Q L + Q+ R
Sbjct: 370 VKIWRDVLELGQSQRLPPEQLKLIGMLYRKHLQ----IILQHRQQQLNKI--QNARMNSQ 423
Query: 755 GAPNVNKL 778
APN NKL
Sbjct: 424 NAPNTNKL 431
>SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 532
Score = 25.8 bits (54), Expect = 7.1
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +3
Query: 72 SIGSPTFSVGVFREE*DGAGCSQAPPVRIQGAHTSGP 182
SI SP+ SVG + D + S + P+ I G + P
Sbjct: 312 SITSPSSSVGQIPQPTDHSALSPSKPMSISGTESPNP 348
>SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 355
Score = 25.8 bits (54), Expect = 7.1
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = +3
Query: 147 PVRIQGAHTSGPGQ*CSRFYVQELEAALLREQ 242
PV +QG HT G +F Q L A +L Q
Sbjct: 29 PVDVQGCHTLGERNDPKKFRFQTLVALMLSSQ 60
>SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 369
Score = 25.4 bits (53), Expect = 9.4
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = -2
Query: 536 LQPREARESFNNVSSSLNERWDASSSNGCCQGRSPLSEVDATVPAPP 396
++P + +S NN +++ S +G + + E+D T P PP
Sbjct: 1 MEPIQKMDSLNNKEGQPDKKRKKHESEK--EGENEVLEIDVTKPVPP 45
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,108,783
Number of Sequences: 5004
Number of extensions: 61149
Number of successful extensions: 190
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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