Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0750
(789 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 118 4e-27
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 118 4e-27
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 31 0.87
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 30 2.0
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 2.7
At3g50110.1 68416.m05478 phosphatase-related similar to PTEN1 GI... 29 3.5
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 29 3.5
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 29 3.5
At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein 29 3.5
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 29 4.6
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 6.1
At4g08960.1 68417.m01476 phosphotyrosyl phosphatase activator (P... 28 8.1
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 118 bits (284), Expect = 4e-27
Identities = 55/91 (60%), Positives = 57/91 (62%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 67 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 126
Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 527
ARGH IE P P
Sbjct: 127 RHAIVSAIAATAVPALVMARGHKIENVPEMP 157
Score = 81.0 bits (191), Expect = 8e-16
Identities = 40/93 (43%), Positives = 58/93 (62%), Gaps = 1/93 (1%)
Frame = +2
Query: 512 IPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKG 691
+PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKG
Sbjct: 153 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKG 212
Query: 692 PLIIFNKDQG-LTRAFPQHPRCGAPNVNKLNLL 787
PL++F + + +AF P +V +LNLL
Sbjct: 213 PLVVFGTEGAKIVKAFRNLPGVELCHVERLNLL 245
Score = 66.1 bits (154), Expect = 3e-11
Identities = 33/63 (52%), Positives = 41/63 (65%), Gaps = 2/63 (3%)
Frame = +1
Query: 76 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 249
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 250 GHK 258
GH+
Sbjct: 65 GHQ 67
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 118 bits (284), Expect = 4e-27
Identities = 55/91 (60%), Positives = 57/91 (62%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 66 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 125
Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 527
ARGH IE P P
Sbjct: 126 RHAIVSAIAATAVPALVMARGHKIENVPEMP 156
Score = 79.8 bits (188), Expect = 2e-15
Identities = 39/93 (41%), Positives = 58/93 (62%), Gaps = 1/93 (1%)
Frame = +2
Query: 512 IPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKG 691
+PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKG
Sbjct: 152 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKG 211
Query: 692 PLIIFNKD-QGLTRAFPQHPRCGAPNVNKLNLL 787
PL+++ + + +AF P +V +LNLL
Sbjct: 212 PLVVYGTEGSKIVKAFRNLPGVELCHVERLNLL 244
Score = 68.1 bits (159), Expect = 6e-12
Identities = 33/66 (50%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
Frame = +1
Query: 67 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 240
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 241 KEAGHK 258
K+AGH+
Sbjct: 61 KKAGHQ 66
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 31.1 bits (67), Expect = 0.87
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -1
Query: 369 HHDTCYRRHPDRTYGYHHHGHAEF 298
HH Y H YGY +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 29.9 bits (64), Expect = 2.0
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = -1
Query: 570 CLVLLISWTLSATTKGSSGIFQ*CV 496
CLVLL+S +A TK SGIF C+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFTGCL 33
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 2.7
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +1
Query: 91 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 237
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At3g50110.1 68416.m05478 phosphatase-related similar to PTEN1
GI:5566292 from [Drosophila melanogaster]; contains
prosite evidence: PS00383: Tyrosine specific protein
phosphatases active site
Length = 632
Score = 29.1 bits (62), Expect = 3.5
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = -2
Query: 527 REARESFNNVSSSLNERWDASSSNGCCQGRSPLSEVDATVPAPPG 393
REA E + +S+ + D + G PLS+ D VP PPG
Sbjct: 42 REAHEDSISTEASV-AKVDDTQMPASSTGSEPLSKTDDIVPCPPG 85
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 29.1 bits (62), Expect = 3.5
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +2
Query: 365 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 463
WW +V LA+ APS P + L SR C
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 497
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 29.1 bits (62), Expect = 3.5
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +2
Query: 365 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 463
WW +V LA+ APS P + L SR C
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 496
>At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein
Length = 320
Score = 29.1 bits (62), Expect = 3.5
Identities = 15/64 (23%), Positives = 32/64 (50%)
Frame = -3
Query: 697 EGSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGLVDLLDFVGYNQGKL 518
E SL +D A F ++ +K + L+ KD+R ++ ++ D++ G + K+
Sbjct: 242 EASLRIDAVASAGFKISRSKLVDLISSKDVRVNWATVTKNGTIVKTGDVVSVSGKGRLKI 301
Query: 517 GNLS 506
G ++
Sbjct: 302 GEIN 305
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 28.7 bits (61), Expect = 4.6
Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
Frame = +3
Query: 216 LEAALLREQGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 386
+ AL+R + G ++ +AE + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRRMG-AKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 387 TKPW 398
W
Sbjct: 135 ISKW 138
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 6.1
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +1
Query: 142 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 237
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
>At4g08960.1 68417.m01476 phosphotyrosyl phosphatase activator
(PTPA) family protein similar to Protein phosphatase 2A,
regulatory subunit B' (PP2A, subunit B', PR53 isoform)
(Phosphotyrosyl phosphatase activator) (PTPA)
(Swiss-Prot:Q28717) [Oryctolagus cuniculus]
Length = 392
Score = 27.9 bits (59), Expect = 8.1
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = -3
Query: 352 PKAP*PDLWVPPPRTRGIRATARPVPHDSALVCDQP 245
P A P++ PPP R IRA A +PH+S + P
Sbjct: 46 PLASFPEM-SPPPNYRPIRAPAINLPHNSQAIILSP 80
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,161,823
Number of Sequences: 28952
Number of extensions: 371926
Number of successful extensions: 1342
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1246
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1330
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1775300800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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