Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0731
(787 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 60 5e-10
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 52 9e-08
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 38 0.001
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 34 0.020
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 33 0.061
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa... 29 1.00
SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 27 3.0
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 27 4.0
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 26 7.0
SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 26 7.0
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 59.7 bits (138), Expect = 5e-10
Identities = 35/91 (38%), Positives = 51/91 (56%), Gaps = 6/91 (6%)
Frame = +3
Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGG 422
D D+DG I N+L SLG+ + EL +M+ E +G I+FT+ LT+ A +M
Sbjct: 22 DRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMK-D 80
Query: 423 SDEDDVVINAFKTFDEEGK--IDSERLRHAL 509
+D ++ V AFK FD++G I E L H L
Sbjct: 81 TDNEEEVREAFKVFDKDGNGYITVEELTHVL 111
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 52.0 bits (119), Expect = 9e-08
Identities = 31/87 (35%), Positives = 45/87 (51%), Gaps = 2/87 (2%)
Frame = +3
Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDED 434
D D DG IG+ D++ SL + ASE ++ M + PIN LT + + S +
Sbjct: 58 DKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPPINLAAFLTAMGSMLCRISPRN 117
Query: 435 DVVINAFKTFD--EEGKIDSERLRHAL 509
D ++ AF TFD + GKI +R AL
Sbjct: 118 D-LLEAFSTFDDTQSGKIPISTMRDAL 143
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 38.3 bits (85), Expect = 0.001
Identities = 20/73 (27%), Positives = 40/73 (54%)
Frame = +3
Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDED 434
D DKDG+I + + + SLG ++ EL ++ E I+ + ++ +N++ E+
Sbjct: 19 DIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVSNKLRETESEE 78
Query: 435 DVVINAFKTFDEE 473
+ I AF+ FD++
Sbjct: 79 E-YIKAFRVFDKD 90
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 34.3 bits (75), Expect = 0.020
Identities = 32/127 (25%), Positives = 57/127 (44%), Gaps = 7/127 (5%)
Frame = +3
Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFANRMSGG 422
D DKD I ++LRA +LG A + E+ +++ + G + + + ++
Sbjct: 47 DSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEKIV-E 105
Query: 423 SDEDDVVINAFKTF--DEEGKIDSERLRHAL*PGATSSPPTNHEA-YDQMDIDDKGYIDT 593
D + + AF+ F DE GKI LR + EA ++ D+D G I+
Sbjct: 106 RDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINE 165
Query: 594 TKLIAML 614
+ IA++
Sbjct: 166 QEFIAIM 172
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 32.7 bits (71), Expect = 0.061
Identities = 15/38 (39%), Positives = 21/38 (55%)
Frame = +3
Query: 240 GIPANDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 353
G D D G+IG +LR SLG S +E+DE++
Sbjct: 82 GFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
Msa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 533
Score = 28.7 bits (61), Expect = 1.00
Identities = 25/71 (35%), Positives = 33/71 (46%)
Frame = -1
Query: 451 ALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMM 272
A I S SS+PP R S V+ A ++S+ FS P ES + S LP +
Sbjct: 272 APIPFSVSSDPPSTM--GRSNSAVQSPSYFAHSLVNSTEFST---PNESLSSLPSILPSI 326
Query: 271 PSLSWSLAGMP 239
PSL A +P
Sbjct: 327 PSLESGKAELP 337
>SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 231
Score = 27.1 bits (57), Expect = 3.0
Identities = 15/36 (41%), Positives = 18/36 (50%)
Frame = -1
Query: 346 SSSSFSDASLPRESKVARKSFLPMMPSLSWSLAGMP 239
SSSS S +S P + +RK P MP L L P
Sbjct: 3 SSSSSSGSSSPASKRSSRKRQTPPMPELPSFLLAFP 38
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 26.6 bits (56), Expect = 4.0
Identities = 24/56 (42%), Positives = 31/56 (55%), Gaps = 2/56 (3%)
Frame = -1
Query: 427 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKSFLPMMPS 266
S P R+A+ +K S VKL + P+A + SS+ S ASL ES R S P PS
Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS--PTKPS 595
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 25.8 bits (54), Expect = 7.0
Identities = 11/33 (33%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = -1
Query: 361 ASPTISSSSFSDASLPRESKVARKSF-LPMMPS 266
+SP++ +++ + P++S V+RKSF P P+
Sbjct: 35 SSPSVPEGTYTVLNNPKQSTVSRKSFSAPTSPT 67
>SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex
subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1522
Score = 25.8 bits (54), Expect = 7.0
Identities = 18/51 (35%), Positives = 27/51 (52%)
Frame = -1
Query: 364 EASPTISSSSFSDASLPRESKVARKSFLPMMPSLSWSLAGMPP*TQRPASV 212
E PTI SS +D+++PR V + S P +P+ + S PP R +V
Sbjct: 135 EQQPTILSS--TDSNIPRPGTV-KSSASPFVPNQNPSAPPPPPQEYRQLNV 182
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,451,350
Number of Sequences: 5004
Number of extensions: 39342
Number of successful extensions: 123
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 381366860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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