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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0731
         (787 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_16337| Best HMM Match : efhand (HMM E-Value=3.7e-16)                98   6e-21
SB_866| Best HMM Match : efhand (HMM E-Value=7.4e-18)                  81   1e-15
SB_10186| Best HMM Match : efhand (HMM E-Value=3e-14)                  75   5e-14
SB_3224| Best HMM Match : efhand (HMM E-Value=0.00096)                 62   7e-10
SB_49196| Best HMM Match : efhand (HMM E-Value=3.4e-39)                57   2e-08
SB_44952| Best HMM Match : efhand (HMM E-Value=3.4e-39)                57   2e-08
SB_36414| Best HMM Match : No HMM Matches (HMM E-Value=.)              56   2e-08
SB_7126| Best HMM Match : efhand (HMM E-Value=2e-26)                   50   3e-06
SB_21457| Best HMM Match : efhand (HMM E-Value=1.7e-29)                49   4e-06
SB_5690| Best HMM Match : efhand (HMM E-Value=7.39998e-41)             49   5e-06
SB_14640| Best HMM Match : No HMM Matches (HMM E-Value=.)              45   6e-05
SB_10944| Best HMM Match : efhand (HMM E-Value=7.4e-24)                31   1.1  
SB_644| Best HMM Match : No HMM Matches (HMM E-Value=.)                29   3.2  
SB_48953| Best HMM Match : NIF3 (HMM E-Value=1.7e-12)                  28   7.5  
SB_4471| Best HMM Match : No HMM Matches (HMM E-Value=.)               28   7.5  
SB_23574| Best HMM Match : efhand (HMM E-Value=2e-05)                  28   9.9  
SB_3682| Best HMM Match : efhand (HMM E-Value=7.4e-05)                 28   9.9  

>SB_16337| Best HMM Match : efhand (HMM E-Value=3.7e-16)
          Length = 168

 Score = 98.3 bits (234), Expect = 6e-21
 Identities = 52/106 (49%), Positives = 72/106 (67%), Gaps = 2/106 (1%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDED 434
           D ++DG I KNDL+A FDSLG+L +++ +++M+ EASGPINFT  LTLF  ++S G+D +
Sbjct: 40  DQNRDGFIDKNDLKAVFDSLGKLVNDEYVEDMLNEASGPINFTMFLTLFGEKIS-GTDPE 98

Query: 435 DVVINAFKTFDEEGK--IDSERLRHAL*PGATSSPPTNHEAYDQMD 566
           DV+ NAF +FD EGK  ID E+L+      + S   T  E  D MD
Sbjct: 99  DVIRNAFGSFDLEGKGSIDEEKLKRLC--MSMSDRMTAEEWEDMMD 142



 Score = 38.7 bits (86), Expect = 0.005
 Identities = 16/29 (55%), Positives = 21/29 (72%)
 Frame = +1

Query: 166 KPQGKRTGSNVFSMFSQKQVAEFKEAFQL 252
           K + +R  SNVF+MF Q Q+ EFKEAF +
Sbjct: 10  KKKAQRATSNVFAMFEQSQIQEFKEAFNM 38


>SB_866| Best HMM Match : efhand (HMM E-Value=7.4e-18)
          Length = 171

 Score = 80.6 bits (190), Expect = 1e-15
 Identities = 47/123 (38%), Positives = 70/123 (56%), Gaps = 3/123 (2%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDED 434
           D + DG I K DL     SLG+  ++  L+EMV E+ GPINFT  LTLF  +++ G+D +
Sbjct: 41  DQNHDGFIDKEDLHDMLASLGKDPTDGYLEEMVKESPGPINFTMFLTLFGEKLN-GTDPE 99

Query: 435 DVVINAFKTFDEE--GKIDSERLRHAL*P-GATSSPPTNHEAYDQMDIDDKGYIDTTKLI 605
           DV+ NAF  FDE+  G+I  + L+ A+   G   S     +      ID +G++D +K  
Sbjct: 100 DVIRNAFACFDEDGNGRIHEDLLKEAMMSMGDRFSDEQVDDILRDAPIDKEGFLDYSKFT 159

Query: 606 AML 614
            +L
Sbjct: 160 RIL 162



 Score = 37.9 bits (84), Expect = 0.009
 Identities = 16/29 (55%), Positives = 20/29 (68%)
 Frame = +1

Query: 166 KPQGKRTGSNVFSMFSQKQVAEFKEAFQL 252
           K +  R  SNVF+MF Q Q+ EFKEAF +
Sbjct: 11  KKRAHRATSNVFAMFDQSQIQEFKEAFNM 39


>SB_10186| Best HMM Match : efhand (HMM E-Value=3e-14)
          Length = 133

 Score = 75.4 bits (177), Expect = 5e-14
 Identities = 37/75 (49%), Positives = 53/75 (70%), Gaps = 1/75 (1%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASG-PINFTQLLTLFANRMSGGSDE 431
           D ++DG I K DL+  + SLG+  +++ LD+M+ EASG  INFT  LTLF  +++ G+D 
Sbjct: 41  DQNRDGFIDKEDLKDMYASLGKSPNDQFLDDMIEEASGSTINFTMFLTLFGEKLN-GTDP 99

Query: 432 DDVVINAFKTFDEEG 476
           +DV+ NAF  FDEEG
Sbjct: 100 EDVIKNAFGCFDEEG 114



 Score = 38.3 bits (85), Expect = 0.007
 Identities = 16/29 (55%), Positives = 21/29 (72%)
 Frame = +1

Query: 166 KPQGKRTGSNVFSMFSQKQVAEFKEAFQL 252
           K + +R  SNVF+MF Q Q+ EFKEAF +
Sbjct: 11  KKRAQRATSNVFAMFDQTQIQEFKEAFNM 39


>SB_3224| Best HMM Match : efhand (HMM E-Value=0.00096)
          Length = 322

 Score = 61.7 bits (143), Expect = 7e-10
 Identities = 36/113 (31%), Positives = 62/113 (54%), Gaps = 3/113 (2%)
 Frame = +3

Query: 285 NDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTF 464
           +DLR T  SLG+  + +E+D+M+ E     NFT  L++F  RM+  +D +D+++ AF   
Sbjct: 178 DDLRGTLSSLGQKPTFEEIDDMM-EGKPSFNFTSFLSMFGLRMA-NTDPEDIILRAFSCL 235

Query: 465 DE--EGKIDSERLRHAL*P-GATSSPPTNHEAYDQMDIDDKGYIDTTKLIAML 614
           D+  +GK+ ++ LR  L   G         + +D +  D  G +D  KL+ +L
Sbjct: 236 DDGGDGKLSAKMLRELLTTMGQRMKYSDVTQLFDDVGADQDGNLDYVKLVRLL 288


>SB_49196| Best HMM Match : efhand (HMM E-Value=3.4e-39)
          Length = 659

 Score = 56.8 bits (131), Expect = 2e-08
 Identities = 37/119 (31%), Positives = 60/119 (50%), Gaps = 7/119 (5%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRMSGG 422
           D D DG I   +L     SLG+  +E EL +M+ E     +G I+F + LT+ A +M   
Sbjct: 10  DKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKN- 68

Query: 423 SDEDDVVINAFKTFDEEGK--IDSERLRHAL*P-GATSSPPTNHEAYDQMDIDDKGYID 590
           +D ++ +  AF+ FD++G   I +  LRH +   G   +     E   + DID  G ++
Sbjct: 69  TDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVN 127



 Score = 32.3 bits (70), Expect = 0.46
 Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 4/48 (8%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQ 386
           D D +G I   +LR    +LG   +++E+DEM+ EA     G +N+ +
Sbjct: 83  DKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYDE 130


>SB_44952| Best HMM Match : efhand (HMM E-Value=3.4e-39)
          Length = 250

 Score = 56.8 bits (131), Expect = 2e-08
 Identities = 37/119 (31%), Positives = 60/119 (50%), Gaps = 7/119 (5%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRMSGG 422
           D D DG I   +L     SLG+  +E EL +M+ E     +G I+F + LT+ A +M   
Sbjct: 10  DKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKN- 68

Query: 423 SDEDDVVINAFKTFDEEGK--IDSERLRHAL*P-GATSSPPTNHEAYDQMDIDDKGYID 590
           +D ++ +  AF+ FD++G   I +  LRH +   G   +     E   + DID  G ++
Sbjct: 69  TDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVN 127



 Score = 32.3 bits (70), Expect = 0.46
 Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 4/48 (8%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQ 386
           D D +G I   +LR    +LG   +++E+DEM+ EA     G +N+ +
Sbjct: 83  DKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYDE 130


>SB_36414| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 299

 Score = 56.4 bits (130), Expect = 2e-08
 Identities = 37/119 (31%), Positives = 60/119 (50%), Gaps = 7/119 (5%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRMSGG 422
           D D DG I   +L     SLG+  +E EL +M+ E     +G I+F + LT+ A +M   
Sbjct: 21  DKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKD- 79

Query: 423 SDEDDVVINAFKTFDEEGK--IDSERLRHAL*P-GATSSPPTNHEAYDQMDIDDKGYID 590
           +D ++ +  AF+ FD++G   I +  LRH +   G   +     E   + DID  G ++
Sbjct: 80  TDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVN 138


>SB_7126| Best HMM Match : efhand (HMM E-Value=2e-26)
          Length = 213

 Score = 49.6 bits (113), Expect = 3e-06
 Identities = 33/101 (32%), Positives = 53/101 (52%), Gaps = 7/101 (6%)
 Frame = +3

Query: 309 SLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFDEEG 476
           SLG+  +E EL +M+ E     +G I+F + LT+ A +M G  D D+ +  AF+ FD++G
Sbjct: 3   SLGQNPTEAELQDMINEVDADGNGIIDFPEFLTMMAKKM-GEQDSDEEIREAFRVFDKDG 61

Query: 477 K--IDSERLRHAL*P-GATSSPPTNHEAYDQMDIDDKGYID 590
              I +  LRH +   G   +     E   + DID  G ++
Sbjct: 62  NGFISAAELRHVMTNLGEKLTDEEVEEMIMEADIDGDGQVN 102


>SB_21457| Best HMM Match : efhand (HMM E-Value=1.7e-29)
          Length = 420

 Score = 49.2 bits (112), Expect = 4e-06
 Identities = 36/119 (30%), Positives = 60/119 (50%), Gaps = 7/119 (5%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRMSGG 422
           D D  G I  ++L     SLG+  +E+EL +M+ E     +G I+F + L + A ++   
Sbjct: 300 DKDGGGSISSSELAHVMRSLGQNPTEQELKDMIAEVDQDDNGEIDFPEFLQMVAKKIKE- 358

Query: 423 SDEDDVVINAFKTFDE--EGKIDSERLRHAL*P-GATSSPPTNHEAYDQMDIDDKGYID 590
           +D ++ + +AFK FD+  +G I S  L+  +   G   +     E   + DID  G ID
Sbjct: 359 TDTEEEIQDAFKVFDKNGDGMISSSELKLVMSNLGERLTDDEVDEMIREADIDGDGMID 417



 Score = 34.3 bits (75), Expect = 0.11
 Identities = 15/50 (30%), Positives = 27/50 (54%)
 Frame = +3

Query: 213 TEAGR*VQGGIPANDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA 362
           T+    +Q      D + DG+I  ++L+    +LG   ++ E+DEM+ EA
Sbjct: 359 TDTEEEIQDAFKVFDKNGDGMISSSELKLVMSNLGERLTDDEVDEMIREA 408


>SB_5690| Best HMM Match : efhand (HMM E-Value=7.39998e-41)
          Length = 153

 Score = 48.8 bits (111), Expect = 5e-06
 Identities = 36/133 (27%), Positives = 59/133 (44%), Gaps = 7/133 (5%)
 Frame = +3

Query: 237 GGIPANDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLLTLFA 404
           GG    D D  G I   +L     SLG+  S++EL +M+ E     +G ++F + L +  
Sbjct: 4   GGFSLFDKDGSGTISNEELEVVMKSLGQNPSDEELQQMIQEVDADGNGEVDFEEFLAMMK 63

Query: 405 NRMSGGSDEDDVVINAFKTFDE--EGKIDSERLRHAL*P-GATSSPPTNHEAYDQMDIDD 575
            +M    D +  +  AF+ FD   +G I    LR  +   G   S     E   + D+D 
Sbjct: 64  KQMQ-HRDAEAEMREAFRVFDRNGDGSISEWELRSVMASLGEKLSDDEIKEMMREADLDG 122

Query: 576 KGYIDTTKLIAML 614
            G I+  + + M+
Sbjct: 123 DGVINFQEFVQMV 135


>SB_14640| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 321

 Score = 45.2 bits (102), Expect = 6e-05
 Identities = 29/91 (31%), Positives = 47/91 (51%), Gaps = 6/91 (6%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFTQLLTLFANRMSGG 422
           D D  G +   +L     +LG   +E+E+ EMV E     +G ++F + + L  N+    
Sbjct: 158 DADGSGHVTTKELHKAMRTLGFNPTEEEIQEMVNEVDYDGNGVLDFNEFVDLMENQKKPD 217

Query: 423 SDEDDVVINAFKTF--DEEGKIDSERLRHAL 509
            +E D+ INAF  F  D++G I++  LR  L
Sbjct: 218 EEEQDL-INAFHVFDSDDKGYIEASELRDLL 247


>SB_10944| Best HMM Match : efhand (HMM E-Value=7.4e-24)
          Length = 127

 Score = 31.1 bits (67), Expect = 1.1
 Identities = 22/77 (28%), Positives = 35/77 (45%), Gaps = 3/77 (3%)
 Frame = +3

Query: 393 TLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERL-RHAL*PGATSSPPTNHEAYDQM 563
           T+ A  +    D  + ++ AFK F  DE GKI  + L R A   G   +     E  D+ 
Sbjct: 45  TIDAKELKSEKDSKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEA 104

Query: 564 DIDDKGYIDTTKLIAML 614
           D D  G I+  + + ++
Sbjct: 105 DRDGDGEINEAEFLRIM 121


>SB_644| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 350

 Score = 29.5 bits (63), Expect = 3.2
 Identities = 34/128 (26%), Positives = 56/128 (43%), Gaps = 6/128 (4%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANRMSG-GSDE 431
           D D +G+I   +++  F  +G   +EKE  E+V +A                M G G   
Sbjct: 98  DKDGNGLISAQEMKFVFTCMGFNITEKEAVELVKQAD---------------MDGDGHIN 142

Query: 432 DDVVINAFKTFDE--EGKIDSERLRHAL*PGATSSPPTNHE---AYDQMDIDDKGYIDTT 596
            +   NAF +FD+  +G+ID+E L   +   +    P + E      Q D D  G ID  
Sbjct: 143 YEEFKNAFMSFDKNVDGRIDAEELE--IVTRSIGLHPKDEELKAMIKQADKDGSGDIDLP 200

Query: 597 KLIAMLTA 620
           + I ++ +
Sbjct: 201 EFIELMAS 208


>SB_48953| Best HMM Match : NIF3 (HMM E-Value=1.7e-12)
          Length = 288

 Score = 28.3 bits (60), Expect = 7.5
 Identities = 18/59 (30%), Positives = 27/59 (45%), Gaps = 1/59 (1%)
 Frame = +3

Query: 348 MVGEASGPINFTQLLTLFANRMSGGSDEDDVVINA-FKTFDEEGKIDSERLRHAL*PGA 521
           M+GE   P+N T+ L    N+M  G      ++N   K     G   S  +++AL  GA
Sbjct: 166 MIGEFETPMNETEFLAFVKNKMQCGGIRHSALLNKNIKKVAVLGGSGSYAIKNALQAGA 224


>SB_4471| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 331

 Score = 28.3 bits (60), Expect = 7.5
 Identities = 12/28 (42%), Positives = 15/28 (53%)
 Frame = +3

Query: 516 GATSSPPTNHEAYDQMDIDDKGYIDTTK 599
           GAT  PP N +  D +D   KGY+   K
Sbjct: 104 GATFLPPANVKVVDSIDWRSKGYVTPVK 131


>SB_23574| Best HMM Match : efhand (HMM E-Value=2e-05)
          Length = 69

 Score = 27.9 bits (59), Expect = 9.9
 Identities = 15/35 (42%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
 Frame = +3

Query: 255 DHDKDGIIGKNDLRATFDSLGRLASEKELDE-MVG 356
           D + +G+IG  +LR    SLG   S++E+D  MVG
Sbjct: 15  DRNGNGLIGAAELRHLLASLGDKLSDEEVDNLMVG 49


>SB_3682| Best HMM Match : efhand (HMM E-Value=7.4e-05)
          Length = 340

 Score = 27.9 bits (59), Expect = 9.9
 Identities = 16/45 (35%), Positives = 29/45 (64%), Gaps = 4/45 (8%)
 Frame = +3

Query: 270 GIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPINFTQLL 392
           G++ +  LRA  D+LGR+A+ +E+ E + E    +SG ++F + L
Sbjct: 164 GVLCRILLRAIPDTLGRMATTEEIKEAMLEVDVDSSGTVDFFEFL 208


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,306,622
Number of Sequences: 59808
Number of extensions: 314928
Number of successful extensions: 1013
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 921
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1008
length of database: 16,821,457
effective HSP length: 81
effective length of database: 11,977,009
effective search space used: 2155861620
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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