BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0728 (708 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces p... 149 3e-37 SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc... 44 2e-05 SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po... 37 0.003 SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 29 0.65 SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 3.5 SPCC18.05c |||notchless-like protein|Schizosaccharomyces pombe|c... 26 4.6 SPBC1215.01 |shy1||SURF-family protein Shy1|Schizosaccharomyces ... 26 4.6 SPAC3A12.15 |vps53||GARP complex subunit Vps53 |Schizosaccharomy... 26 6.1 >SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 149 bits (362), Expect = 3e-37 Identities = 75/85 (88%), Positives = 79/85 (92%) Frame = +1 Query: 256 MSDKMGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSV 435 MS K GGGSLTALPVIETQ GDVSAYIPTNVISITDGQIFLE+ELF+KGIRPAINVGLSV Sbjct: 340 MSPKHGGGSLTALPVIETQGGDVSAYIPTNVISITDGQIFLESELFFKGIRPAINVGLSV 399 Query: 436 SRVGSAAQTKAMKQVAGSMKLELAR 510 SRVGSAAQ KAMKQVAG +KL LA+ Sbjct: 400 SRVGSAAQVKAMKQVAGQIKLFLAQ 424 Score = 126 bits (305), Expect = 2e-30 Identities = 56/65 (86%), Positives = 64/65 (98%) Frame = +3 Query: 6 YTIIVSATASDAAPLQYLAPYSGCAMGEFFRDNGKHALIIYDDLSKQAVAYRQMSLLLRR 185 Y+IIV+ATAS++APLQYLAP+SGCAMGE+FRDNGKH L++YDDLSKQAVAYRQMSLLLRR Sbjct: 256 YSIIVAATASESAPLQYLAPFSGCAMGEWFRDNGKHGLVVYDDLSKQAVAYRQMSLLLRR 315 Query: 186 PPGRE 200 PPGRE Sbjct: 316 PPGRE 320 Score = 101 bits (241), Expect = 1e-22 Identities = 44/68 (64%), Positives = 57/68 (83%) Frame = +3 Query: 501 IGSYREVAAFAQFGSDLDAATQQLLNRGMRLTELLKQGQYVPMAIEEQVAIIYCGVRGHL 680 + YREVA+FAQFGSDLDA T+ L+RG+RLTELLKQ QY P+A+EEQV +IYCGV+G+L Sbjct: 422 LAQYREVASFAQFGSDLDAGTRATLDRGLRLTELLKQPQYSPLAVEEQVPLIYCGVKGYL 481 Query: 681 DKLDPSKI 704 DK+ ++ Sbjct: 482 DKIPVDRV 489 Score = 41.5 bits (93), Expect = 1e-04 Identities = 18/22 (81%), Positives = 19/22 (86%) Frame = +2 Query: 203 YPGDVFYLHSRLLERAARCLTK 268 YPGDVFYLHSRLLERAA+ K Sbjct: 322 YPGDVFYLHSRLLERAAKMSPK 343 >SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces pombe|chr 1|||Manual Length = 503 Score = 44.4 bits (100), Expect = 2e-05 Identities = 24/65 (36%), Positives = 38/65 (58%) Frame = +1 Query: 277 GSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAA 456 GS+T +P++ D++ IP IT+GQIF++ +L I P INV S+SR+ +A Sbjct: 324 GSITQIPILTMPNDDITHPIPDLTGYITEGQIFVDRQLHNNAIYPPINVLPSLSRLMKSA 383 Query: 457 QTKAM 471 + M Sbjct: 384 IGEGM 388 Score = 27.9 bits (59), Expect = 1.5 Identities = 17/57 (29%), Positives = 25/57 (43%), Gaps = 1/57 (1%) Frame = +3 Query: 30 ASDAAPLQYLAPYSGCAMGEFFR-DNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGR 197 A+D + + P + EF KH L I D++ A A R++S PGR Sbjct: 242 ANDPTIERIITPRLALSASEFLAYQTEKHVLTILTDMTSYADALREVSAAREEVPGR 298 >SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces pombe|chr 1|||Manual Length = 619 Score = 36.7 bits (81), Expect = 0.003 Identities = 18/61 (29%), Positives = 34/61 (55%) Frame = +3 Query: 9 TIIVSATASDAAPLQYLAPYSGCAMGEFFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRP 188 T +V+ T++ + + Y+G + E++RD GK+ ++ D S+ A A R++S L Sbjct: 317 TTLVANTSNMPVAAREASIYTGITLAEYYRDQGKNVSMMADSTSRWAEALREISGRLAEM 376 Query: 189 P 191 P Sbjct: 377 P 377 >SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 29.1 bits (62), Expect = 0.65 Identities = 15/35 (42%), Positives = 20/35 (57%), Gaps = 1/35 (2%) Frame = -3 Query: 574 SSCCVAASKSEPN-WAKAATSRYEPIPASWNRPPA 473 +S V++S S P WAK A+S PA W +P A Sbjct: 412 ASTSVSSSTSSPAPWAKPASSAAPSNPAPWQQPAA 446 >SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 682 Score = 26.6 bits (56), Expect = 3.5 Identities = 11/19 (57%), Positives = 15/19 (78%) Frame = +3 Query: 135 LSKQAVAYRQMSLLLRRPP 191 LSK+A+ YR+ L L+RPP Sbjct: 102 LSKRALHYRRKRLALKRPP 120 >SPCC18.05c |||notchless-like protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 502 Score = 26.2 bits (55), Expect = 4.6 Identities = 14/35 (40%), Positives = 18/35 (51%) Frame = +1 Query: 514 VRSQLLPSSVLTWMPLHSSCSTEECVLLSSSSKDN 618 +R P L W PLH + + LL+S SKDN Sbjct: 216 LRRHTKPIMALCWQPLHLAPDSGP-YLLASGSKDN 249 >SPBC1215.01 |shy1||SURF-family protein Shy1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 26.2 bits (55), Expect = 4.6 Identities = 23/80 (28%), Positives = 32/80 (40%) Frame = +3 Query: 447 ICCSDQGYEAGGRFHEAGIGSYREVAAFAQFGSDLDAATQQLLNRGMRLTELLKQGQYVP 626 + C DQ G R E G Y V F LD + L+NRG +Q P Sbjct: 104 VFCHDQEMLVGPRTKEGQPG-YHVVTPFI-----LDDGRRILVNRGWIARSFAEQSSRDP 157 Query: 627 MAIEEQVAIIYCGVRGHLDK 686 ++ + +I +R H DK Sbjct: 158 SSLPKGPVVIEGLLRQHTDK 177 >SPAC3A12.15 |vps53||GARP complex subunit Vps53 |Schizosaccharomyces pombe|chr 1|||Manual Length = 756 Score = 25.8 bits (54), Expect = 6.1 Identities = 14/45 (31%), Positives = 25/45 (55%) Frame = -2 Query: 668 DTAVNDGDLFLNSHGHILSLLEELSKTHSSVEQLLCSGIQVRTEL 534 D ++ G+ + + I+SLL+ELS S E S +Q+ +E+ Sbjct: 65 DAKLSMGEKMDDLNSSIVSLLQELSTLQSVAENTQSSIVQMTSEI 109 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,976,240 Number of Sequences: 5004 Number of extensions: 60357 Number of successful extensions: 184 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 171 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 184 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 329179816 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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