Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0725
(742 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 56 5e-09
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 54 2e-08
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 53 5e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 42 9e-05
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 36 0.008
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 29 0.70
SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 28 1.2
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 2.1
SPCC1795.01c |mad3|SPCC895.02|mitotic spindle checkpoint protein... 27 2.8
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 3.7
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 4.9
SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc... 25 8.6
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 56.0 bits (129), Expect = 5e-09
Identities = 26/75 (34%), Positives = 44/75 (58%)
Frame = +3
Query: 285 ISFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDE 464
+ E FL + + +F++G + FDKD G I ELR++L++LGEKLS++E
Sbjct: 55 VDMEQFLQVLNRPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEE 114
Query: 465 VEQLLQGQEDSQGNI 509
+++LL+G G +
Sbjct: 115 MDELLKGVPVKDGMV 129
Score = 46.4 bits (105), Expect = 4e-06
Identities = 19/40 (47%), Positives = 27/40 (67%)
Frame = +1
Query: 139 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKNV 258
+++AF LFD G G+I IGD LRA GQNPT +++ +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEI 47
Score = 29.1 bits (62), Expect = 0.70
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +1
Query: 136 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKNVL 261
EF + FQ+FD G I V ++ L +LG+ + ++ +L
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 54.4 bits (125), Expect = 2e-08
Identities = 28/74 (37%), Positives = 45/74 (60%)
Frame = +3
Query: 285 ISFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDE 464
I F FL + K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +E
Sbjct: 65 IDFTEFLTMMAR--KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122
Query: 465 VEQLLQGQEDSQGN 506
V +++ + D+ G+
Sbjct: 123 VADMIR-EADTDGD 135
Score = 50.4 bits (115), Expect = 3e-07
Identities = 20/48 (41%), Positives = 37/48 (77%)
Frame = +1
Query: 118 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKNVL 261
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT +++++++
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMI 54
Score = 34.7 bits (76), Expect = 0.014
Identities = 15/46 (32%), Positives = 29/46 (63%)
Frame = +1
Query: 124 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKNVL 261
D E +EAF++FD G+G I V ++ L +LG+ ++ +V +++
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMI 127
Score = 32.3 bits (70), Expect = 0.075
Identities = 17/54 (31%), Positives = 33/54 (61%)
Frame = +3
Query: 354 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNITM 515
+F E FD+D +G I+S EL ++ +LG+ + E++ ++ + D+ GN T+
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMI-NEVDADGNGTI 65
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 52.8 bits (121), Expect = 5e-08
Identities = 22/53 (41%), Positives = 37/53 (69%)
Frame = +3
Query: 324 SKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 482
+K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ ++Q
Sbjct: 69 NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQ 121
Score = 39.9 bits (89), Expect = 4e-04
Identities = 18/44 (40%), Positives = 29/44 (65%)
Frame = +1
Query: 118 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 249
S++Q E +EAF L+D DG I + +G LR+LG N T++++
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAEL 47
Score = 31.9 bits (69), Expect = 0.099
Identities = 12/39 (30%), Positives = 25/39 (64%)
Frame = +1
Query: 136 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 252
E+ +AF++FD G I A+ D ++ LG+ ++++V+
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQ 117
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 41.9 bits (94), Expect = 9e-05
Identities = 24/59 (40%), Positives = 35/59 (59%), Gaps = 1/59 (1%)
Frame = +1
Query: 118 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKNVLFILNLM-KGYL 291
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V +L + KGYL
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYL 90
Score = 29.9 bits (64), Expect = 0.40
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +1
Query: 124 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKNVL 261
D L E + AF+LFD GKI + + + L +N + +++ ++
Sbjct: 107 DPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMI 152
Score = 27.9 bits (59), Expect = 1.6
Identities = 15/60 (25%), Positives = 34/60 (56%), Gaps = 2/60 (3%)
Frame = +3
Query: 351 NDFIEGLRHFDKDGNGFISSAE-LRHLLSTLGEKLSDDEVEQLLQ-GQEDSQGNITMRTL 524
++ ++ LR FDK G G++ + +R + + E+ +E+++ + +D G I++R L
Sbjct: 73 SEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRNL 132
Score = 25.4 bits (53), Expect = 8.6
Identities = 14/43 (32%), Positives = 20/43 (46%)
Frame = +3
Query: 354 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 482
D E + FD D + I ELR + LG EV ++L+
Sbjct: 38 DINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILR 80
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 35.5 bits (78), Expect = 0.008
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +1
Query: 109 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKNVLFILN 273
A + Q+ E +EAF L D GDG I + L +L Q+ +E + ++ +N
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
Score = 35.1 bits (77), Expect = 0.011
Identities = 15/44 (34%), Positives = 26/44 (59%)
Frame = +3
Query: 351 NDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 482
ND +E FD +G I + +R LS++G+++ EVE +L+
Sbjct: 117 NDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILR 160
Score = 32.7 bits (71), Expect = 0.056
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +1
Query: 145 EAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKNVL 261
EAF FD GKI ++ + DAL ++G +V+++L
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESIL 159
Score = 29.9 bits (64), Expect = 0.40
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +3
Query: 363 EGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 482
E DKDG+G I +++ +L++L + S+D + + +
Sbjct: 52 EAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFE 91
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 29.1 bits (62), Expect = 0.70
Identities = 19/57 (33%), Positives = 29/57 (50%)
Frame = -3
Query: 470 LYLIITELLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 300
L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2493
Score = 28.3 bits (60), Expect = 1.2
Identities = 16/39 (41%), Positives = 23/39 (58%)
Frame = -3
Query: 521 SSHSYISLRVFLSLQELLYLIITELLSESREQVSQFRRR 405
+SH+ + L V L + + L L+IT L SE E SQ R +
Sbjct: 1134 ASHTAVKLPVLLYILDTLNLVITHLQSEESE--SQLREK 1170
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.5 bits (58), Expect = 2.1
Identities = 20/66 (30%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = +3
Query: 294 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK-LSDD 461
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K L++
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 462 EVEQLL 479
+E +L
Sbjct: 702 LLEHIL 707
>SPCC1795.01c |mad3|SPCC895.02|mitotic spindle checkpoint protein
Mad3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 310
Score = 27.1 bits (57), Expect = 2.8
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = -3
Query: 302 KHLKRYPFIRFKMKSTFFTSDSVGFCPKALNAS 204
K +K PF+RF+ K FT + F P++ +++
Sbjct: 177 KRMKAKPFLRFQQKYQQFTHRWLEFAPQSFSSN 209
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 26.6 bits (56), Expect = 3.7
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +1
Query: 136 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKNVL 261
E +EAF LFD G I + + LG+N T+ ++ +L
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54
Score = 26.2 bits (55), Expect = 4.9
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +3
Query: 378 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL 479
FD G+I +LR + LGE L+ ++++ +L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 26.2 bits (55), Expect = 4.9
Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -1
Query: 217 LLTHLRSERHVSFHLHEN--RRAGRLLEILPIDPLNIQPYYESTNEIEGNEIGIFKGCDL 44
+ TH+ SE L+E R ++ +LP+ P +++P G + K D+
Sbjct: 216 IFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDI 275
Query: 43 SKSN 32
K+N
Sbjct: 276 IKAN 279
>SPBC23E6.07c |rfc1||DNA replication factor C complex subunit
Rfc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 934
Score = 25.4 bits (53), Expect = 8.6
Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = +3
Query: 441 GEKLSDDEVEQLLQG-QEDSQGNITMRTLFTSSC 539
G KLS V+QL+QG Q D + I + + + SC
Sbjct: 558 GLKLSPQAVDQLVQGTQSDMRQIINLLSTYKLSC 591
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,933,469
Number of Sequences: 5004
Number of extensions: 59021
Number of successful extensions: 205
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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