SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0717
         (843 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g48520.2 68414.m05423 glutamyl-tRNA(Gln) amidotransferase B f...    33   0.18 
At1g48520.3 68414.m05424 glutamyl-tRNA(Gln) amidotransferase B f...    32   0.41 
At1g48520.1 68414.m05425 glutamyl-tRNA(Gln) amidotransferase B f...    32   0.41 
At5g41090.1 68418.m04995 no apical meristem (NAM) family protein...    29   3.9  
At4g00450.1 68417.m00062 expressed protein                             29   5.1  
At4g25540.1 68417.m03682 DNA mismatch repair protein MSH3 (MSH3)...    28   8.9  

>At1g48520.2 68414.m05423 glutamyl-tRNA(Gln) amidotransferase B
           family protein contains Pfam profiles: PF02934 PET112
           family, N terminal region, PF02637 GatB/Yqey domain,
           PF01162 PET112 family, C terminal region
          Length = 475

 Score = 33.5 bits (73), Expect = 0.18
 Identities = 17/71 (23%), Positives = 33/71 (46%)
 Frame = +3

Query: 297 NVIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKNLKLPLNKVHIVGFNLGAHVAGVTGR 476
           +V V ++    +   +   +A   + S IA +LKN KL +N + +    L   +A +   
Sbjct: 394 DVSVAEYFDAVIGKGAEVKLAANWIMSDIAAYLKNEKLSINDIKLTPQELAELIAAIKDG 453

Query: 477 NLEGKVARITG 509
            + GK+ +  G
Sbjct: 454 TISGKIGKEVG 464


>At1g48520.3 68414.m05424 glutamyl-tRNA(Gln) amidotransferase B
           family protein contains Pfam profiles: PF02934 PET112
           family, N terminal region, PF02637 GatB/Yqey domain,
           PF01162 PET112 family, C terminal region
          Length = 488

 Score = 32.3 bits (70), Expect = 0.41
 Identities = 16/68 (23%), Positives = 32/68 (47%)
 Frame = +3

Query: 297 NVIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKNLKLPLNKVHIVGFNLGAHVAGVTGR 476
           +V V ++    +   +   +A   + S IA +LKN KL +N + +    L   +A +   
Sbjct: 394 DVSVAEYFDAVIGKGAEVKLAANWIMSDIAAYLKNEKLSINDIKLTPQELAELIAAIKDG 453

Query: 477 NLEGKVAR 500
            + GK+ +
Sbjct: 454 TISGKIGK 461


>At1g48520.1 68414.m05425 glutamyl-tRNA(Gln) amidotransferase B
           family protein contains Pfam profiles: PF02934 PET112
           family, N terminal region, PF02637 GatB/Yqey domain,
           PF01162 PET112 family, C terminal region
          Length = 550

 Score = 32.3 bits (70), Expect = 0.41
 Identities = 16/68 (23%), Positives = 32/68 (47%)
 Frame = +3

Query: 297 NVIVVDWSSFSLSTYSTAVMAVTGVGSSIATFLKNLKLPLNKVHIVGFNLGAHVAGVTGR 476
           +V V ++    +   +   +A   + S IA +LKN KL +N + +    L   +A +   
Sbjct: 394 DVSVAEYFDAVIGKGAEVKLAANWIMSDIAAYLKNEKLSINDIKLTPQELAELIAAIKDG 453

Query: 477 NLEGKVAR 500
            + GK+ +
Sbjct: 454 TISGKIGK 461


>At5g41090.1 68418.m04995 no apical meristem (NAM) family protein
           contains Pfam PF02365: No apical meristem (NAM) domain;
           similar to unknown protein (emb|CAB88055.1)
          Length = 212

 Score = 29.1 bits (62), Expect = 3.9
 Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
 Frame = +1

Query: 73  QNLERFGSLNKYYYYSNAQRNPITLTEDH---FPTG--NDPAAPFNNNWDIVVIIH 225
           ++L  +GS   +Y+Y    +N +T T+ +    PTG   D   P  N  ++V I H
Sbjct: 53  RDLPGYGSEEHWYFYCKKPKNQVTRTKSYNLWIPTGEKTDVLDPKKNGGELVGIKH 108


>At4g00450.1 68417.m00062 expressed protein
          Length = 2124

 Score = 28.7 bits (61), Expect = 5.1
 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = +2

Query: 479  FGRQSSKNYGLDPSARDWENNVLRLGTNDAQYVEVIHT-DGSGVNKNGLGIAIG 637
            + R   K YG  PS  +W NN  +  +      E+ HT  G+G   N LG+  G
Sbjct: 1059 YTRYILKRYGSLPSVVEWHNN-FKATSEKKLLSELDHTRSGNGEYGNPLGVPAG 1111


>At4g25540.1 68417.m03682 DNA mismatch repair protein MSH3 (MSH3)
           identical to SP|O65607 DNA mismatch repair protein MSH3
           (AtMsh3) {Arabidopsis thaliana}
          Length = 1081

 Score = 27.9 bits (59), Expect = 8.9
 Identities = 15/57 (26%), Positives = 23/57 (40%)
 Frame = +2

Query: 536 NNVLRLGTNDAQYVEVIHTDGSGVNKNGLGIAIGHIDFFVNGRLVQPGCTNNLCSHN 706
           N  + L  N  Q +EV+  +  G     L   + H       RL++   T+ LC  N
Sbjct: 417 NTEMTLSANTLQQLEVVKNNSDGSESGSLFHNMNHTLTVYGSRLLRHWVTHPLCDRN 473


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,246,991
Number of Sequences: 28952
Number of extensions: 380520
Number of successful extensions: 868
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 847
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 868
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1950880000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -