Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0708
(703 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 143 9e-35
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 143 9e-35
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 143 9e-35
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.74
At1g29760.1 68414.m03639 expressed protein 31 0.74
At5g63000.1 68418.m07904 expressed protein 30 1.7
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 3.0
At4g38880.1 68417.m05506 amidophosphoribosyltransferase, putativ... 28 6.9
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 27 9.1
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 27 9.1
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 27 9.1
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 143 bits (347), Expect = 9e-35
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +1
Query: 1 ARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
ARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPAG 252
+ I+ QR I+VDGKVRTD TYPAG
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPAG 85
Score = 132 bits (319), Expect = 2e-31
Identities = 71/147 (48%), Positives = 85/147 (57%)
Frame = +3
Query: 255 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSPGTCV*SREAVTWGAWAPSCPARDIPAPST 614
IRYPDPLIK ND+I+LD+ KI++ G V G
Sbjct: 147 IRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFE 206
Query: 615 LCTSRTPRDTPSATRLNNVFIIGKGTK 695
+ ATRL NV+ IGKGTK
Sbjct: 207 TIHIQDSTGHEFATRLGNVYTIGKGTK 233
Score = 73.3 bits (172), Expect = 1e-13
Identities = 30/51 (58%), Positives = 39/51 (76%)
Frame = +2
Query: 509 FIKFESGNLCMITGSRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFGNEV 661
FIKF+ GN+ M+TG RN GRVG I +RE+H GSF+ +HI+DSTGH F +
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRL 222
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 143 bits (347), Expect = 9e-35
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +1
Query: 1 ARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
ARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPAG 252
+ I+ QR I+VDGKVRTD TYPAG
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPAG 85
Score = 132 bits (318), Expect = 3e-31
Identities = 71/147 (48%), Positives = 85/147 (57%)
Frame = +3
Query: 255 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSPGTCV*SREAVTWGAWAPSCPARDIPAPST 614
IRYPDPLIK ND+I+LD+ KI++ G V G
Sbjct: 147 IRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFE 206
Query: 615 LCTSRTPRDTPSATRLNNVFIIGKGTK 695
+ ATRL NV+ IGKGTK
Sbjct: 207 TIHIQDSTGHEFATRLGNVYTIGKGTK 233
Score = 73.3 bits (172), Expect = 1e-13
Identities = 30/51 (58%), Positives = 39/51 (76%)
Frame = +2
Query: 509 FIKFESGNLCMITGSRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFGNEV 661
FIKF+ GN+ M+TG RN GRVG I +RE+H GSF+ +HI+DSTGH F +
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRL 222
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 143 bits (347), Expect = 9e-35
Identities = 65/84 (77%), Positives = 73/84 (86%)
Frame = +1
Query: 1 ARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEV 180
ARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV
Sbjct: 2 ARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREV 61
Query: 181 LKIVKQRLIKVDGKVRTDPTYPAG 252
+ I+ QR I+VDGKVRTD TYPAG
Sbjct: 62 ISILMQRHIQVDGKVRTDKTYPAG 85
Score = 132 bits (318), Expect = 3e-31
Identities = 71/147 (48%), Positives = 85/147 (57%)
Frame = +3
Query: 255 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 434
MDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRT
Sbjct: 87 MDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRT 146
Query: 435 IRYPDPLIKVNDSIQLDIATTKIMDSSSLSPGTCV*SREAVTWGAWAPSCPARDIPAPST 614
IRYPDPLIK ND+I+LD+ KI++ G V G
Sbjct: 147 IRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFE 206
Query: 615 LCTSRTPRDTPSATRLNNVFIIGKGTK 695
+ ATRL NV+ IGKGTK
Sbjct: 207 TIHIQDSTGHEFATRLGNVYTIGKGTK 233
Score = 73.3 bits (172), Expect = 1e-13
Identities = 30/51 (58%), Positives = 39/51 (76%)
Frame = +2
Query: 509 FIKFESGNLCMITGSRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFGNEV 661
FIKF+ GN+ M+TG RN GRVG I +RE+H GSF+ +HI+DSTGH F +
Sbjct: 172 FIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRL 222
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.74
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -3
Query: 122 KHSRNLWGPVDGLGAYTPPSLSNIHAL 42
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.74
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -3
Query: 233 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 141
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At5g63000.1 68418.m07904 expressed protein
Length = 201
Score = 29.9 bits (64), Expect = 1.7
Identities = 22/58 (37%), Positives = 29/58 (50%)
Frame = -1
Query: 670 TLFNLVAEGVSRGVLDVHNVEGAGMSLAGHDGAHAPQVTASRDHTQVPGLKLDESIIF 497
+L N +AE +RGV DV NV GAG + A G P A R + G L ++ F
Sbjct: 102 SLQNFLAE--TRGVHDVFNVVGAGSATAAVFGLIMPGSLAWRARNVLLGSVLGATVCF 157
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -3
Query: 212 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 96
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At4g38880.1 68417.m05506 amidophosphoribosyltransferase, putative /
glutamine phosphoribosylpyrophosphate amidotransferase,
putative / phosphoribosyldiphosphate 5-amidotransferase,
putative similar to amidophosphoribosyltransferase
[Arabidopsis thaliana] GI:469195; contains Pfam profiles
PF00156: Phosphoribosyl transferase domain, PF00310:
Glutamine amidotransferases class-II
Length = 532
Score = 27.9 bits (59), Expect = 6.9
Identities = 24/80 (30%), Positives = 33/80 (41%)
Frame = +2
Query: 398 ECSVLGNA*WPHHPLSRPTYQSQRFHPVRHCNYEDYGFIKFESGNLCMITGSRNLGRVGT 577
EC V+G P SR +Y + H ++H E G + L ITG + V T
Sbjct: 76 ECGVVGIHGDPE--ASRLSYLA--LHALQHRGQEGAGIVAANQNGLESITGVGLVSDVFT 131
Query: 578 IVSRERHPGSFDIVHIKDST 637
PG I H++ ST
Sbjct: 132 ESKLNNLPGDIAIGHVRYST 151
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 9.1
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 27.5 bits (58), Expect = 9.1
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 27.5 bits (58), Expect = 9.1
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 145 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 243
N ++A+T NE+ +K+RL D ++R D Y
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 736
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,107,731
Number of Sequences: 28952
Number of extensions: 385145
Number of successful extensions: 1161
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1105
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1160
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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