BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0698 (498 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to ... 29 1.3 At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to ... 29 1.3 At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma mem... 27 7.0 At2g04240.2 68415.m00413 zinc finger (C3HC4-type RING finger) fa... 27 7.0 At2g04240.1 68415.m00412 zinc finger (C3HC4-type RING finger) fa... 27 7.0 At1g35540.1 68414.m04413 transcriptional factor B3 family protei... 27 7.0 At2g02480.1 68415.m00187 DNA polymerase-related weak similarity ... 27 9.3 >At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to nuclear protein ZAP, Mus musculus, EMBL:AB033168 this cDNA provides a truncated ORF likely due to a skipped exon. An alternative ORF is provided. Length = 383 Score = 29.5 bits (63), Expect = 1.3 Identities = 14/48 (29%), Positives = 29/48 (60%) Frame = -2 Query: 170 IGTNKIPTINNR*PATYPAIPPKSNASYEISAYRNTIIIMAGVIRPQH 27 + TN PTI ++YP +P S +S +++ R+ +I ++ +++P H Sbjct: 224 VTTNSSPTIPPS--SSYPQMPNASPSSAQLAPTRSKVIDVSHLLKPPH 269 >At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to nuclear protein ZAP, Mus musculus, EMBL:AB033168 this cDNA provides a truncated ORF likely due to a skipped exon. An alternative ORF is provided. Length = 661 Score = 29.5 bits (63), Expect = 1.3 Identities = 14/48 (29%), Positives = 29/48 (60%) Frame = -2 Query: 170 IGTNKIPTINNR*PATYPAIPPKSNASYEISAYRNTIIIMAGVIRPQH 27 + TN PTI ++YP +P S +S +++ R+ +I ++ +++P H Sbjct: 224 VTTNSSPTIPPS--SSYPQMPNASPSSAQLAPTRSKVIDVSHLLKPPH 269 >At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma membrane-type, putative / Ca2+-ATPase, putative (ACA7) identical to SP|O64806 Potential calcium-transporting ATPase 7, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 7) {Arabidopsis thaliana}; strong similarity to SP|O81108 Calcium-transporting ATPase 2, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 2) {Arabidopsis thaliana} Length = 1015 Score = 27.1 bits (57), Expect = 7.0 Identities = 19/44 (43%), Positives = 24/44 (54%) Frame = -3 Query: 352 TCVIREGLSFSRNTFASSLNESQVLRSPADLGPKIPWSGKKALE 221 T + + GLSF+ TFA L + +R LGP WSG ALE Sbjct: 354 TIIGKIGLSFAIVTFAV-LVQGMFMRK-LSLGPHWWWSGDDALE 395 >At2g04240.2 68415.m00413 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 162 Score = 27.1 bits (57), Expect = 7.0 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = +2 Query: 71 CTH*FHTKHCSSEEWPDMWLVTC 139 C H FH K C E+W D W +TC Sbjct: 121 CGHLFH-KTCL-EKWIDYWNITC 141 >At2g04240.1 68415.m00412 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 162 Score = 27.1 bits (57), Expect = 7.0 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = +2 Query: 71 CTH*FHTKHCSSEEWPDMWLVTC 139 C H FH K C E+W D W +TC Sbjct: 121 CGHLFH-KTCL-EKWIDYWNITC 141 >At1g35540.1 68414.m04413 transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-related contains Pfam profiles: PF02362 B3 DNA binding domain, PF02309: AUX/IAA family Length = 605 Score = 27.1 bits (57), Expect = 7.0 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -3 Query: 334 GLSFSRNTFASSLNESQVLRSPADLGPK 251 GL SR T +SQ+LRSP ++ K Sbjct: 477 GLEISRLTQEKKFGQSQILRSPTEIQSK 504 >At2g02480.1 68415.m00187 DNA polymerase-related weak similarity to DNA polymerase III holoenzyme tau subunit [Thermus thermophilus] GI:2583049 Length = 1218 Score = 26.6 bits (56), Expect = 9.3 Identities = 19/68 (27%), Positives = 31/68 (45%), Gaps = 4/68 (5%) Frame = -2 Query: 257 TQDSLERKESFR-VSMKFPVLYLYNVRQMEIGTNKIPTINNR*P---ATYPAIPPKSNAS 90 T++ L E R V+ P+L + ++++ NNR TY + P S +S Sbjct: 207 TEEELSNSEDLRKVTGASPLLLKLKQKNWSRSSSRLLRANNRKEDSSCTYNSTPALSTSS 266 Query: 89 YEISAYRN 66 Y + A RN Sbjct: 267 YNMYAVRN 274 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,870,856 Number of Sequences: 28952 Number of extensions: 218228 Number of successful extensions: 543 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 536 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 543 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 878448512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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