BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0689 (758 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC328.10c |rps502|rps5-2|40S ribosomal protein S5|Schizosaccha... 140 2e-34 SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces p... 140 2e-34 SPAC16E8.10c |||mitochondrial ribosomal protein subunit S7|Schiz... 38 0.002 SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 0.72 SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosa... 29 0.95 SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces... 27 3.8 SPAC631.02 |||bromodomain protein|Schizosaccharomyces pombe|chr ... 26 5.1 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 26 6.7 SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe... 25 8.9 SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 25 8.9 >SPAC328.10c |rps502|rps5-2|40S ribosomal protein S5|Schizosaccharomyces pombe|chr 1|||Manual Length = 203 Score = 140 bits (339), Expect = 2e-34 Identities = 69/91 (75%), Positives = 77/91 (84%) Frame = +3 Query: 252 SGRYAHKRFRKAQCPIVERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVL 431 +GR+ KRFRKA+C IVERLTNSLMM+GRNNGKKL+A RIVKHAFEII LLT +NPLQVL Sbjct: 52 AGRFQTKRFRKARCFIVERLTNSLMMNGRNNGKKLLATRIVKHAFEIIALLTDQNPLQVL 111 Query: 432 VTAIINSGPREDSTRIGRAGTVRRQALMFHP 524 V A+ GPREDSTRIG AGTVRRQA+ P Sbjct: 112 VDAVAACGPREDSTRIGSAGTVRRQAVDVSP 142 Score = 98.7 bits (235), Expect = 8e-22 Identities = 46/57 (80%), Positives = 54/57 (94%) Frame = +2 Query: 536 NQAIWLLCTGAREAAFRNIKTIAECVADELINAAKGSSNSYAIKKKDELERVAKSNR 706 NQA+ L+ GAREAAFRN+K+I+EC+A+E+INAAKGSSNSYAIKKKDELERVAKSNR Sbjct: 147 NQALALITIGAREAAFRNVKSISECLAEEIINAAKGSSNSYAIKKKDELERVAKSNR 203 Score = 35.1 bits (77), Expect = 0.011 Identities = 21/61 (34%), Positives = 34/61 (55%) Frame = +1 Query: 88 AGSVVVETMSLPQAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAAGMH 267 A S++ + +SL + IKLF ++ V+V D+SL DYI++ + LPH+A Sbjct: 2 AASIIPKEVSLDETG---HIKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQ 56 Query: 268 T 270 T Sbjct: 57 T 57 >SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces pombe|chr 1|||Manual Length = 203 Score = 140 bits (339), Expect = 2e-34 Identities = 69/91 (75%), Positives = 77/91 (84%) Frame = +3 Query: 252 SGRYAHKRFRKAQCPIVERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVL 431 +GR+ KRFRKA+C IVERLTNSLMM+GRNNGKKL+A RIVKHAFEII LLT +NPLQVL Sbjct: 52 AGRFQTKRFRKARCFIVERLTNSLMMNGRNNGKKLLATRIVKHAFEIIALLTDQNPLQVL 111 Query: 432 VTAIINSGPREDSTRIGRAGTVRRQALMFHP 524 V A+ GPREDSTRIG AGTVRRQA+ P Sbjct: 112 VDAVAACGPREDSTRIGSAGTVRRQAVDVSP 142 Score = 98.7 bits (235), Expect = 8e-22 Identities = 46/57 (80%), Positives = 54/57 (94%) Frame = +2 Query: 536 NQAIWLLCTGAREAAFRNIKTIAECVADELINAAKGSSNSYAIKKKDELERVAKSNR 706 NQA+ L+ GAREAAFRN+K+I+EC+A+E+INAAKGSSNSYAIKKKDELERVAKSNR Sbjct: 147 NQALALITIGAREAAFRNVKSISECLAEEIINAAKGSSNSYAIKKKDELERVAKSNR 203 Score = 32.7 bits (71), Expect = 0.058 Identities = 21/57 (36%), Positives = 30/57 (52%), Gaps = 3/57 (5%) Frame = +1 Query: 109 TMSLPQAADIPE---IKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAAGMHT 270 T SL + E IKLF ++ V+V D+SL DYI++ + LPH+A T Sbjct: 3 TSSLTPGVSLDENGSIKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQT 57 >SPAC16E8.10c |||mitochondrial ribosomal protein subunit S7|Schizosaccharomyces pombe|chr 1|||Manual Length = 259 Score = 37.5 bits (83), Expect = 0.002 Identities = 23/53 (43%), Positives = 28/53 (52%) Frame = +3 Query: 300 VERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVLVTAIINSGP 458 V+ L N +M +GKK A +IV A II TGENP+ VL AI P Sbjct: 126 VQHLVNLIM----RDGKKAKAEKIVATALSIIQKETGENPIDVLKQAIAEISP 174 >SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 468 Score = 29.1 bits (62), Expect = 0.72 Identities = 14/33 (42%), Positives = 17/33 (51%) Frame = +2 Query: 359 GRTYCQTCV*NYSLVNWRKPSASTRDCHYQLWT 457 G TYC C L+NW K S S C +L+T Sbjct: 101 GHTYCYEC-----LLNWLKESKSCPTCRQKLYT 128 >SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 707 Score = 28.7 bits (61), Expect = 0.95 Identities = 12/22 (54%), Positives = 14/22 (63%) Frame = -3 Query: 552 SQIAWLTRRKGETSTLDDELYP 487 SQ AWL+R KG S L +YP Sbjct: 445 SQAAWLSREKGVASELQSLVYP 466 >SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces pombe|chr 1|||Manual Length = 567 Score = 26.6 bits (56), Expect = 3.8 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = +3 Query: 633 QLRVHLTPTPSKRRTSWSVLLNPTV 707 Q ++H P P +RR S + L NP++ Sbjct: 124 QQKIHRNPQPRRRRRSTTALPNPSL 148 >SPAC631.02 |||bromodomain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 727 Score = 26.2 bits (55), Expect = 5.1 Identities = 18/63 (28%), Positives = 30/63 (47%), Gaps = 1/63 (1%) Frame = +3 Query: 432 VTAIINSGPREDSTRIGRAGT-VRRQALMFHPCAESTKQSGFCAQVHVRLHSEILKQSQS 608 VT+ G R S + G RRQA M+ + +++ + + H R + +K QS Sbjct: 340 VTSYSRPGRRPRSMTAPKGGARTRRQAAMYSNSSSGIRETMYDLKPHRRKDAAEMKFCQS 399 Query: 609 VLQ 617 VL+ Sbjct: 400 VLK 402 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 25.8 bits (54), Expect = 6.7 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = +2 Query: 308 PYKLSNDARSEQWQKTDGRTYCQTCV*NYSLVNWRKPSAST 430 PYK+ + + W + G+TY + + + L R+ +AST Sbjct: 138 PYKIVEHSNGDAWLEARGKTYSPSQIGGFILSKMRE-TAST 177 >SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 25.4 bits (53), Expect = 8.9 Identities = 15/45 (33%), Positives = 26/45 (57%) Frame = +1 Query: 124 QAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAA 258 + ADI + FGR ++++++ + I+V+EKYAK P A Sbjct: 7 KVADI-SLAAFGR---KELEIAENEMPGLIAVREKYAKSQPLKGA 47 >SPBC2G2.08 |ade9||C-1- tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr ogenase/methylenetetrahydrofolatecyclohydrolase/formylte trahydrofolatesynthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 969 Score = 25.4 bits (53), Expect = 8.9 Identities = 9/33 (27%), Positives = 22/33 (66%) Frame = +1 Query: 187 SDMSLQDYISVKEKYAKYLPHSAAGMHTSVSVK 285 SDM ++ + ++K +Y+K +P++ + T ++K Sbjct: 706 SDMGMEKFFNIKCRYSKLVPNTVVLVTTVKALK 738 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,079,762 Number of Sequences: 5004 Number of extensions: 62327 Number of successful extensions: 159 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 149 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 159 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 363302114 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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