Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0687
(733 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 153 3e-38
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 152 4e-38
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 81 2e-16
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 49 8e-07
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.22
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.6
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 4.8
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 4.8
SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.4
SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 26 6.4
SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 26 6.4
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 8.4
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 153 bits (370), Expect = 3e-38
Identities = 68/84 (80%), Positives = 75/84 (89%)
Frame = +3
Query: 3 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEIT 182
Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE SV EIT
Sbjct: 233 QVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEIT 292
Query: 183 NACFEPANQMVKCDPRHGKYMACC 254
N CFEP NQMVKCDPR G+YMA C
Sbjct: 293 NQCFEPYNQMVKCDPRAGRYMATC 316
Score = 148 bits (358), Expect = 9e-37
Identities = 64/85 (75%), Positives = 75/85 (88%)
Frame = +2
Query: 254 LLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVC 433
LLYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++AKV RAVC
Sbjct: 317 LLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVC 376
Query: 434 MLSNTTAIAEAWARLDHKFDLMYAK 508
MLSNTT+IAEAW+RLDHKFDLMY+K
Sbjct: 377 MLSNTTSIAEAWSRLDHKFDLMYSK 401
Score = 79.8 bits (188), Expect = 4e-16
Identities = 35/37 (94%), Positives = 36/37 (97%)
Frame = +1
Query: 511 AFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGMDS 621
AFVHWYVGEGMEEGEFSEAREDLAALE+DYEEVG DS
Sbjct: 403 AFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 439
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 152 bits (369), Expect = 4e-38
Identities = 65/85 (76%), Positives = 77/85 (90%)
Frame = +2
Query: 254 LLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVC 433
LLYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV RAVC
Sbjct: 321 LLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVC 380
Query: 434 MLSNTTAIAEAWARLDHKFDLMYAK 508
MLSNTT+IAEAW+RLDHKFDLMY+K
Sbjct: 381 MLSNTTSIAEAWSRLDHKFDLMYSK 405
Score = 150 bits (363), Expect = 2e-37
Identities = 67/84 (79%), Positives = 74/84 (88%)
Frame = +3
Query: 3 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEIT 182
Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE SV EIT
Sbjct: 237 QVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEIT 296
Query: 183 NACFEPANQMVKCDPRHGKYMACC 254
N CFEP NQMVKCDPR G+YMA C
Sbjct: 297 NQCFEPYNQMVKCDPRTGRYMATC 320
Score = 79.8 bits (188), Expect = 4e-16
Identities = 35/37 (94%), Positives = 36/37 (97%)
Frame = +1
Query: 511 AFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGMDS 621
AFVHWYVGEGMEEGEFSEAREDLAALE+DYEEVG DS
Sbjct: 407 AFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 443
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 80.6 bits (190), Expect = 2e-16
Identities = 33/80 (41%), Positives = 52/80 (65%)
Frame = +3
Query: 6 IVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITN 185
+++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ + + + +SV E+T
Sbjct: 232 VMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQ 291
Query: 186 ACFEPANQMVKCDPRHGKYM 245
F+ N MV DPRHG+Y+
Sbjct: 292 QMFDANNMMVAADPRHGRYL 311
Score = 47.2 bits (107), Expect = 2e-06
Identities = 21/42 (50%), Positives = 29/42 (69%), Gaps = 3/42 (7%)
Frame = +1
Query: 502 RQGAFVHWYVGEGMEEGEFSEAR---EDLAALEKDYEEVGMD 618
R+ AF+HWY GEGM+E EF+EA DL + + Y+E G+D
Sbjct: 390 RRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAGID 431
Score = 42.7 bits (96), Expect = 5e-05
Identities = 25/84 (29%), Positives = 43/84 (51%)
Frame = +2
Query: 257 LYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCM 436
L+RG V K+V+ I +++TK + FV+W P + PP DL + +
Sbjct: 316 LFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK-----DL---KMSATF 367
Query: 437 LSNTTAIAEAWARLDHKFDLMYAK 508
+ N+T+I E + RL +F M+ +
Sbjct: 368 IGNSTSIQEIFRRLGDQFSAMFRR 391
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 48.8 bits (111), Expect = 8e-07
Identities = 25/77 (32%), Positives = 40/77 (51%), Gaps = 3/77 (3%)
Frame = +3
Query: 6 IVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---AEKAYHEQLSVAE 176
++S+ T +LR+ G +N DL +L+P PR HF L +Y P + E + +V +
Sbjct: 235 VMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLD 294
Query: 177 ITNACFEPANQMVKCDP 227
+ P NQMV +P
Sbjct: 295 VMRRLLLPKNQMVSVNP 311
Score = 37.5 bits (83), Expect = 0.002
Identities = 20/82 (24%), Positives = 43/82 (52%)
Frame = +2
Query: 248 LLLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRA 427
+L + +G+ P DV+ ++ I+ +R F+ W P +V ++ + P + ++ +
Sbjct: 320 ILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGL--- 376
Query: 428 VCMLSNTTAIAEAWARLDHKFD 493
ML+N T+IA + R ++D
Sbjct: 377 --MLANHTSIASLFKRTLDQYD 396
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 30.7 bits (66), Expect = 0.22
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = +3
Query: 264 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 398
VV P + RP++P P LS V PV+ V + PP P
Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.9 bits (59), Expect = 1.6
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = +1
Query: 64 PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 210
P+++ P S + T +SS+P P N+ PSP S + AS+ P R
Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 26.2 bits (55), Expect = 4.8
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 127 QSSLPRRPTMNSFPSPRSQTHASSPPTR 210
+ SLPRRP+ +P S T ++ PP +
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 4.8
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = +3
Query: 300 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 386
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 751
Score = 25.8 bits (54), Expect = 6.4
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = +1
Query: 61 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 201
SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P
Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664
>SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 793
Score = 25.8 bits (54), Expect = 6.4
Identities = 18/64 (28%), Positives = 24/64 (37%)
Frame = +2
Query: 233 WQVHGLLLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLA 412
W+ Y + KDVN + TI +W PTG ++ Y V D
Sbjct: 141 WRHSSFAQYYLYNTETKDVNM-LGQDNEHWTISLAEWSPTGHQLSFVYNNDLYVRKND-G 198
Query: 413 KVQR 424
VQR
Sbjct: 199 NVQR 202
>SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr
1|||Manual
Length = 474
Score = 25.8 bits (54), Expect = 6.4
Identities = 16/51 (31%), Positives = 31/51 (60%)
Frame = +1
Query: 88 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 240
LTP + S +S + +S +R ++N+ PS + +H+SS T + PV+++
Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.4 bits (53), Expect = 8.4
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = +3
Query: 297 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 392
P PS+P+++SN ST G+Q V V + W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,132,095
Number of Sequences: 5004
Number of extensions: 67960
Number of successful extensions: 255
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 229
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 255
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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