Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0681
         (693 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   145   6e-36
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   145   6e-36
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   145   6e-36
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    45   9e-06
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    32   0.090

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  145 bits (351), Expect = 6e-36
 Identities = 64/83 (77%), Positives = 73/83 (87%)
 Frame = +2

Query: 2   FNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 181
           FNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIA
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367

Query: 182 CKFAEIKEKVDRRTGKSTEVNPK 250
           CKFAE+ EK+DRR+GK  E +PK
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPK 390



 Score = 85.8 bits (203), Expect = 5e-18
 Identities = 39/51 (76%), Positives = 44/51 (86%)
 Frame = +1

Query: 244 PKIHKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 396
           PK  KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  145 bits (351), Expect = 6e-36
 Identities = 64/83 (77%), Positives = 73/83 (87%)
 Frame = +2

Query: 2   FNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 181
           FNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIA
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367

Query: 182 CKFAEIKEKVDRRTGKSTEVNPK 250
           CKFAE+ EK+DRR+GK  E +PK
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPK 390



 Score = 85.8 bits (203), Expect = 5e-18
 Identities = 39/51 (76%), Positives = 44/51 (86%)
 Frame = +1

Query: 244 PKIHKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 396
           PK  KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  145 bits (351), Expect = 6e-36
 Identities = 64/83 (77%), Positives = 73/83 (87%)
 Frame = +2

Query: 2   FNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 181
           FNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIA
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367

Query: 182 CKFAEIKEKVDRRTGKSTEVNPK 250
           CKFAE+ EK+DRR+GK  E +PK
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPK 390



 Score = 85.8 bits (203), Expect = 5e-18
 Identities = 39/51 (76%), Positives = 44/51 (86%)
 Frame = +1

Query: 244 PKIHKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 396
           PK  KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 45.2 bits (102), Expect = 9e-06
 Identities = 23/68 (33%), Positives = 37/68 (54%)
 Frame = +2

Query: 29  ELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEK 208
           +++ GYV   +KN P      F AQ+ +L  P  ++ GY+ V+  HTA     FA++  K
Sbjct: 540 DVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHK 598

Query: 209 VDRRTGKS 232
           +D+   KS
Sbjct: 599 LDKTNRKS 606



 Score = 41.1 bits (92), Expect = 1e-04
 Identities = 20/53 (37%), Positives = 30/53 (56%), Gaps = 1/53 (1%)
 Frame = +1

Query: 244 PKIHKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 399
           P     G   I  L    P+C+E F+++  +GRF +RD   TVAVG V+K ++
Sbjct: 610 PMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 31.9 bits (69), Expect = 0.090
 Identities = 15/31 (48%), Positives = 20/31 (64%)
 Frame = +1

Query: 298 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 390
           PLC+   +E P LGRF +R    TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,789,364
Number of Sequences: 5004
Number of extensions: 55993
Number of successful extensions: 168
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)