Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0681
(693 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 134 4e-32
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 134 4e-32
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 134 4e-32
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 134 4e-32
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 78 6e-15
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 44 1e-04
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 35 0.045
At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein /... 29 2.9
At1g71970.1 68414.m08319 expressed protein 29 2.9
At1g55420.1 68414.m06339 DC1 domain-containing protein contains ... 29 3.9
At2g04740.1 68415.m00484 ankyrin repeat family protein contains ... 28 5.1
At1g53300.1 68414.m06041 thioredoxin family protein contains Pfa... 28 5.1
At5g44670.1 68418.m05473 expressed protein contains Pfam:PF01697... 28 6.8
At1g55380.1 68414.m06334 DC1 domain-containing protein contains ... 28 6.8
At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing prote... 27 8.9
At4g11590.1 68417.m01857 F-box family protein contains weak hit ... 27 8.9
At1g05800.1 68414.m00606 lipase class 3 family protein similar t... 27 8.9
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 134 bits (325), Expect = 4e-32
Identities = 58/83 (69%), Positives = 72/83 (86%)
Frame = +2
Query: 2 FNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 181
FNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA
Sbjct: 298 FNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIA 357
Query: 182 CKFAEIKEKVDRRTGKSTEVNPK 250
KF+EI K+DRR+GK E PK
Sbjct: 358 VKFSEILTKIDRRSGKEIEKEPK 380
Score = 83.0 bits (196), Expect = 2e-16
Identities = 36/53 (67%), Positives = 46/53 (86%)
Frame = +1
Query: 241 QPKIHKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 399
+PK K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 378 EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 134 bits (325), Expect = 4e-32
Identities = 58/83 (69%), Positives = 72/83 (86%)
Frame = +2
Query: 2 FNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 181
FNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA
Sbjct: 298 FNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIA 357
Query: 182 CKFAEIKEKVDRRTGKSTEVNPK 250
KF+EI K+DRR+GK E PK
Sbjct: 358 VKFSEILTKIDRRSGKEIEKEPK 380
Score = 83.0 bits (196), Expect = 2e-16
Identities = 36/53 (67%), Positives = 46/53 (86%)
Frame = +1
Query: 241 QPKIHKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 399
+PK K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 378 EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 134 bits (325), Expect = 4e-32
Identities = 58/83 (69%), Positives = 72/83 (86%)
Frame = +2
Query: 2 FNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 181
FNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA
Sbjct: 298 FNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIA 357
Query: 182 CKFAEIKEKVDRRTGKSTEVNPK 250
KF+EI K+DRR+GK E PK
Sbjct: 358 VKFSEILTKIDRRSGKEIEKEPK 380
Score = 83.0 bits (196), Expect = 2e-16
Identities = 36/53 (67%), Positives = 46/53 (86%)
Frame = +1
Query: 241 QPKIHKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 399
+PK K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 378 EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 134 bits (325), Expect = 4e-32
Identities = 58/83 (69%), Positives = 72/83 (86%)
Frame = +2
Query: 2 FNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 181
FNVKNV+VK+L+RGYVA +SK++P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA
Sbjct: 298 FNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIA 357
Query: 182 CKFAEIKEKVDRRTGKSTEVNPK 250
KF+EI K+DRR+GK E PK
Sbjct: 358 VKFSEILTKIDRRSGKEIEKEPK 380
Score = 83.0 bits (196), Expect = 2e-16
Identities = 36/53 (67%), Positives = 46/53 (86%)
Frame = +1
Query: 241 QPKIHKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVN 399
+PK K+GDA +V + P+KP+ VE+F E+PPLGRFAVRDMRQTVAVGVIK+V+
Sbjct: 378 EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVD 430
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 77.8 bits (183), Expect = 6e-15
Identities = 32/52 (61%), Positives = 44/52 (84%)
Frame = +1
Query: 241 QPKIHKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 396
+PK K+ +AAI+N+ P+KP+ VE++ +PPLGRFA+RDMRQTV VGVIK+V
Sbjct: 44 EPKFLKNSEAAIINMTPTKPMVVEAYSAYPPLGRFAIRDMRQTVGVGVIKSV 95
Score = 72.1 bits (169), Expect = 3e-13
Identities = 31/46 (67%), Positives = 35/46 (76%)
Frame = +2
Query: 113 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPK 250
+NH GQI NGYTPVLDCHT+HIA KF+EI K+D RTG E PK
Sbjct: 1 MNHLGQIKNGYTPVLDCHTSHIAVKFSEILTKIDWRTGHEIEKEPK 46
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 44.0 bits (99), Expect = 1e-04
Identities = 17/44 (38%), Positives = 28/44 (63%)
Frame = +1
Query: 256 KSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 387
K+G A + + + +C+E F +FP LGRF +R +T+AVG +
Sbjct: 479 KNGAAVVCRIQVTNSICIEKFSDFPQLGRFTLRTEGKTIAVGKV 522
Score = 32.7 bits (71), Expect = 0.24
Identities = 19/76 (25%), Positives = 35/76 (46%), Gaps = 2/76 (2%)
Frame = +2
Query: 8 VKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN--HPGQISNGYTPVLDCHTAHIA 181
+ + +++ G+V S NP +F AQ+ +L + GY +L H
Sbjct: 395 ITGIEDEDILSGFVLS-SIVNPVPAVTEFVAQLQILELLDNAIFTAGYKAILHIHAVVEE 453
Query: 182 CKFAEIKEKVDRRTGK 229
C+ E+K ++D +T K
Sbjct: 454 CEIIELKSQIDLKTRK 469
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 35.1 bits (77), Expect = 0.045
Identities = 16/51 (31%), Positives = 29/51 (56%)
Frame = +1
Query: 244 PKIHKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 396
P+ + +A++ + P+CVE+F E LGR +R +TVA+G + +
Sbjct: 613 PRCLTAKQSAMLEVSLQNPVCVETFSESRALGRVFLRSSGRTVAMGKVTRI 663
>At4g18180.1 68417.m02701 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Salix gilgiana] GI:6714524; contains
PF00295: Glycosyl hydrolases family 28
(polygalacturonases)
Length = 414
Score = 29.1 bits (62), Expect = 2.9
Identities = 21/71 (29%), Positives = 33/71 (46%)
Frame = -3
Query: 247 WVDFSRFTSTTVNFFFDFCKFAGNVGSVAIQDWCVTV*DLTRMVKHNDLSCKICSTLRWV 68
W + +S++VN +F+ VGS+ C V +LT VK + K S W+
Sbjct: 58 WDKACKSSSSSVNLIIPRGEFS--VGSLRFSGPCTNVSNLTVRVKASTDLSKYRSGGGWI 115
Query: 67 VFGVTSNITTT 35
FG + +T T
Sbjct: 116 QFGWINGLTLT 126
>At1g71970.1 68414.m08319 expressed protein
Length = 225
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = -2
Query: 515 VNEYRSCMKNCAVNSSSYFLPLVAFSAALVTLPP 414
VN Y SC + CAV S +LP +A+ L P
Sbjct: 188 VNLYGSCRRTCAVTESIVYLPRARKAASYDNLLP 221
>At1g55420.1 68414.m06339 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 725
Score = 28.7 bits (61), Expect = 3.9
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = +2
Query: 566 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 664
I+ K C+ V LFY ++ N S FCY C+L+C
Sbjct: 619 IYLKPCHIFKVGLFYKEVEIARNDGNSRLFCYICRLRC 656
>At2g04740.1 68415.m00484 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 578
Score = 28.3 bits (60), Expect = 5.1
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = -2
Query: 521 LSVNEYRSCMKNCAVNSSSYFLPLVAFSAALVTLPPPASLKLTALMTPTATVCLMS 354
L + EY C+ A N + F+ F A L+TLPPP+ P+A +M+
Sbjct: 466 LKIREY--CLDLVACNFEA-FVETHEFRAMLLTLPPPSGDSSLRTTVPSAPGAMMT 518
>At1g53300.1 68414.m06041 thioredoxin family protein contains Pfam
profiles PF00085: Thioredoxin, PF00515: TPR Domain;
similar to tetratricopeptide repeat protein 2
(GI:7248701) [Drosophila melanogaster]; similar to DnaJ
homolog subfamily C member 7 (Tetratricopeptide repeat
protein 2) (TPR repeat protein 2) (Swiss-Prot:Q99615)
[Homo sapiens]
Length = 699
Score = 28.3 bits (60), Expect = 5.1
Identities = 16/50 (32%), Positives = 25/50 (50%)
Frame = -2
Query: 512 NEYRSCMKNCAVNSSSYFLPLVAFSAALVTLPPPASLKLTALMTPTATVC 363
N R + +SSS+ PL++ S++ T P P S A + PT +C
Sbjct: 98 NNLRPRSDSATTSSSSHSQPLLSSSSSSATSPAPTS---PANVLPTGNIC 144
>At5g44670.1 68418.m05473 expressed protein contains Pfam:PF01697
Domain of unknown function
Length = 519
Score = 27.9 bits (59), Expect = 6.8
Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
Frame = -2
Query: 551 SL*PFFPSKH-LSVNEYRSCMKNCAVNSSS 465
+L PF PS +S +E R C+ AVNS+S
Sbjct: 41 TLLPFLPSSFSISASELRFCISRIAVNSTS 70
>At1g55380.1 68414.m06334 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 661
Score = 27.9 bits (59), Expect = 6.8
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = +2
Query: 566 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 664
I+ K C+ V L+Y ++ N S FCY C+L+C
Sbjct: 579 IYLKPCHIFKVGLYYKEVEIARNDGNSRLFCYTCELRC 616
>At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing protein
similar to cell death suppressor protein lls1 from Zea
mays [gi:1935909], Rieske iron-sulfur protein Tic55 from
Pisum sativum [gi:2764524]; contains Pfam PF00355 Rieske
[2Fe-2S] domain
Length = 536
Score = 27.5 bits (58), Expect = 8.9
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +2
Query: 185 KFAEIKEKVDRRTGKSTEVNPK 250
+F + KEK+DR GK E+N K
Sbjct: 258 RFPKPKEKIDREGGKPLEINVK 279
>At4g11590.1 68417.m01857 F-box family protein contains weak hit to
Pfam PF00646: F-box domain
Length = 397
Score = 27.5 bits (58), Expect = 8.9
Identities = 22/82 (26%), Positives = 35/82 (42%)
Frame = +2
Query: 32 LRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKV 211
+ GY NP G + +V + Q GY PV D + + C +KEK+
Sbjct: 130 ISHGYGLEKFITNPSTGKSTVLGRVQTNSKVAQCFLGYDPVNDEYKLLVLC----MKEKM 185
Query: 212 DRRTGKSTEVNPKSTSLEMQPL 277
R G+ T + S+ ++ PL
Sbjct: 186 QRHDGR-TRILQLSSQHQVFPL 206
>At1g05800.1 68414.m00606 lipase class 3 family protein similar to
DEFECTIVE IN ANTHER DEHISCENCE1 [Arabidopsis thaliana]
GI:16215706; contains Pfam profile PF01764: Lipase
Length = 471
Score = 27.5 bits (58), Expect = 8.9
Identities = 18/71 (25%), Positives = 37/71 (52%)
Frame = -2
Query: 524 HLSVNEYRSCMKNCAVNSSSYFLPLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 345
H S++++ S K V+SS+ P+ S++ ++LP +S + A +SR
Sbjct: 30 HFSISQHTS--KRLVVSSSTMSPPI---SSSPLSLPSSSSSQAIPPSRAPAVTLPLSRVW 84
Query: 344 KRPRGGNSWKD 312
+ +G N+W++
Sbjct: 85 REIQGSNNWEN 95
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,676,139
Number of Sequences: 28952
Number of extensions: 300310
Number of successful extensions: 881
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 852
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 881
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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