Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0661
(697 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g47880.1 68418.m05915 eukaryotic peptide chain release factor... 32 0.42
At3g18680.2 68416.m02373 aspartate/glutamate/uridylate kinase fa... 30 1.3
At2g30920.1 68415.m03769 hexaprenyldihydroxybenzoate methyltrans... 29 3.9
At3g26990.1 68416.m03377 expressed protein contains Pfam domain,... 28 5.1
At3g26618.1 68416.m03325 eukaryotic release factor 1 family prot... 28 5.1
At1g12920.1 68414.m01500 eukaryotic release factor 1 family prot... 28 5.1
At5g59020.1 68418.m07393 expressed protein 28 6.8
At2g35090.1 68415.m04305 hypothetical protein 28 6.8
At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID... 28 6.8
At1g54970.1 68414.m06278 proline-rich family protein similar to ... 28 6.8
At5g62640.1 68418.m07862 proline-rich family protein contains pr... 27 9.0
At1g14070.1 68414.m01664 xyloglucan fucosyltransferase, putative... 27 9.0
>At5g47880.1 68418.m05915 eukaryotic peptide chain release factor
subunit 1-1 (ERF1-1) identical to SP|Q39097 Eukaryotic
peptide chain release factor subunit 1-1 (eRF1-1)
(Eukaryotic release factor 1-1) (Omnipotent suppressor
protein 1 homolog 1) (SUP1 homolog 1) {Arabidopsis
thaliana}, eukaryotic release factor 1 homolog
GI:1155261 from [Arabidopsis thaliana]; contains Pfam
profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2,
PF03465 eRF1 domain 3
Length = 436
Score = 31.9 bits (69), Expect = 0.42
Identities = 28/99 (28%), Positives = 44/99 (44%)
Frame = +2
Query: 50 SLSTQANNVMNAVITNTQGLLEDYYQSTSQSEDTCFYVPTLNPTSPPVFRGQCETITGVQ 229
++ A + N + L+ Y++ SQ DT YV + T + G ET+ +
Sbjct: 268 AIELSAEILANVKFIQEKRLIGKYFEEISQ--DTGKYVFGVEDTLNALESGAIETLIVWE 325
Query: 230 NFDVQRYLVGGMKLSGTQLRVTQGSVSAHSTQHQETNTK 346
N D+ RY+ MK S T +V H + QE NT+
Sbjct: 326 NLDINRYV---MKNS-----ATGETVIKHLNKEQEANTE 356
>At3g18680.2 68416.m02373 aspartate/glutamate/uridylate kinase
family protein similar to UMP-kinase GB:CAB38122
gi:4468612 from [Lactococcus lactis] ; contains Pfam
profile PF00696: Amino acid kinase family
Length = 366
Score = 30.3 bits (65), Expect = 1.3
Identities = 18/50 (36%), Positives = 25/50 (50%)
Frame = +2
Query: 11 NRRVGSWKLSRTTSLSTQANNVMNAVITNTQGLLEDYYQSTSQSEDTCFY 160
N+R SW + R NN+M I TQ LLE Y + +S ++C Y
Sbjct: 319 NQRRESWYIDRRNLELHCYNNLMKKPILKTQNLLE--YLRSGESCNSCIY 366
>At2g30920.1 68415.m03769 hexaprenyldihydroxybenzoate
methyltransferase identical to
hexaprenyldihydroxybenzoate methyltransferase SP:O49354
from [Arabidopsis thaliana]
Length = 322
Score = 28.7 bits (61), Expect = 3.9
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = +2
Query: 542 REDSKARQLTAAAQNAMTPIITANRVLHQELYLTVD 649
R + R L +A + + +PII +R+LHQ L+ T D
Sbjct: 6 RVNQLQRLLLSARRLSSSPIIPPSRLLHQRLFSTSD 41
>At3g26990.1 68416.m03377 expressed protein contains Pfam domain,
PF04818: Protein of unknown function, DUF618
Length = 513
Score = 28.3 bits (60), Expect = 5.1
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +1
Query: 85 GHNEHPGSTGRLLSIYQPKRRHLLLCTHTQSYLTP 189
G+N P T L S+ P + L H QSYL+P
Sbjct: 372 GNNNPPAVTETLSSVDFPPEKRPKLQNHDQSYLSP 406
>At3g26618.1 68416.m03325 eukaryotic release factor 1 family protein
/ eRF1 family protein contains Pfam profiles: PF03463
eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
domain 3
Length = 435
Score = 28.3 bits (60), Expect = 5.1
Identities = 18/67 (26%), Positives = 30/67 (44%)
Frame = +2
Query: 50 SLSTQANNVMNAVITNTQGLLEDYYQSTSQSEDTCFYVPTLNPTSPPVFRGQCETITGVQ 229
++ A + N + L+ Y++ SQ DT YV + T + G ET+ +
Sbjct: 267 AIELSAEILSNVKFIQEKKLIGKYFEEISQ--DTGKYVFGVEDTLKALEMGAVETLIVWE 324
Query: 230 NFDVQRY 250
N D+ RY
Sbjct: 325 NLDINRY 331
>At1g12920.1 68414.m01500 eukaryotic release factor 1 family protein
/ eRF1 family protein contains Pfam profiles: PF03463
eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
domain 3
Length = 434
Score = 28.3 bits (60), Expect = 5.1
Identities = 18/67 (26%), Positives = 30/67 (44%)
Frame = +2
Query: 50 SLSTQANNVMNAVITNTQGLLEDYYQSTSQSEDTCFYVPTLNPTSPPVFRGQCETITGVQ 229
++ A + N + L+ Y++ SQ DT YV + T + G ET+ +
Sbjct: 266 AIELSAEILSNVKFIQEKKLIGKYFEEISQ--DTGKYVFGVEDTLKALEMGAIETLIVWE 323
Query: 230 NFDVQRY 250
N D+ RY
Sbjct: 324 NLDINRY 330
>At5g59020.1 68418.m07393 expressed protein
Length = 780
Score = 27.9 bits (59), Expect = 6.8
Identities = 11/19 (57%), Positives = 11/19 (57%)
Frame = +1
Query: 61 SSKQRHERGHNEHPGSTGR 117
S K HER NEH G GR
Sbjct: 195 SEKNLHERNRNEHDGELGR 213
>At2g35090.1 68415.m04305 hypothetical protein
Length = 124
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/31 (38%), Positives = 20/31 (64%)
Frame = +2
Query: 56 STQANNVMNAVITNTQGLLEDYYQSTSQSED 148
STQA + A+ + + ++E YQSTS +E+
Sbjct: 44 STQAEALTGAITSGLESVVEKEYQSTSVAEE 74
>At1g67120.1 68414.m07636 midasin-related similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q12019)
[Saccharomyces cerevisiae]; similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo
sapiens]; contains Prosite PS00017: ATP/GTP-binding site
motif A (P-loop)
Length = 5336
Score = 27.9 bits (59), Expect = 6.8
Identities = 19/49 (38%), Positives = 27/49 (55%), Gaps = 2/49 (4%)
Frame = +1
Query: 49 VVEHSSKQRHERGHNEHPGSTGRLLSIYQPKR--RHLLLCTHTQSYLTP 189
++E S Q+H+ + E LL YQP +HLLL THT+S + P
Sbjct: 4019 LLESSGLQKHKFENIEMSNHFKGLL--YQPAYDPKHLLLLTHTKSNIHP 4065
>At1g54970.1 68414.m06278 proline-rich family protein similar to
proline-rich protein GI:170048 from [Glycine max]
Length = 335
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +3
Query: 99 PRVYWKTTINLPAKAKTPAFMYPHSILPHPLY 194
P VY K T+ P K+P++ P P P Y
Sbjct: 168 PPVYTKPTLPPPVYKKSPSYSPPPPFAPKPTY 199
>At5g62640.1 68418.m07862 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 520
Score = 27.5 bits (58), Expect = 9.0
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +2
Query: 143 EDTCFYVPTLNPTSPP 190
ED+ +Y PTLNPT P
Sbjct: 123 EDSVYYHPTLNPTGAP 138
>At1g14070.1 68414.m01664 xyloglucan fucosyltransferase, putative
(FUT7) nearly identical to SP|Q9XI81 Probable
fucosyltransferase 7 (EC 2.4.1.-) (AtFUT7) {Arabidopsis
thaliana}; similar to xyloglucan fucosyltransferase
GI:5231145 from (Arabidopsis thaliana)
Length = 509
Score = 27.5 bits (58), Expect = 9.0
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = +1
Query: 94 EHPGSTGRLLSIYQPKR 144
EHP +TG ++ +YQP R
Sbjct: 375 EHPTTTGDIVEVYQPSR 391
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,481,463
Number of Sequences: 28952
Number of extensions: 321421
Number of successful extensions: 915
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 885
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 915
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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