BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= mg--0657 (693 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub... 92 8e-20 SPAC23H3.09c |gly1||threonine aldolase |Schizosaccharomyces pomb... 27 1.9 SPBC839.06 |cta3||P-type ATPase, calcium transporting Cta3|Schiz... 27 1.9 SPBC336.01 |fbh1|fdh1, fdh|DNA helicase I|Schizosaccharomyces po... 27 2.6 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 25 7.9 SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 25 7.9 SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 25 7.9 >SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 91.9 bits (218), Expect = 8e-20 Identities = 44/86 (51%), Positives = 58/86 (67%) Frame = +1 Query: 1 HKLDQGPATSATLTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYSGQEAVAVG 180 +K+D P+T +T + L LYE++ +RR+E A LYK K IRGFCHL GQEAVA G Sbjct: 62 YKIDV-PSTEIEVTKGELLGLYEKMVTIRRLELACDALYKAKKIRGFCHLSIGQEAVAAG 120 Query: 181 MRAAMRDADSVITAYRCHGWTYLMGL 258 + A+ DS+IT+YRCHG+ Y GL Sbjct: 121 IEGAITLDDSIITSYRCHGFAYTRGL 146 Score = 91.5 bits (217), Expect = 1e-19 Identities = 36/58 (62%), Positives = 48/58 (82%) Frame = +3 Query: 246 SHGVSVLGVLSELTGRRTGCSRGKGGSMHLYGRNFYGGNGIVGAQVPLGAGVAFAHKY 419 + G+S+ ++ EL GR+ G S+GKGGSMH++ +NFYGGNGIVGAQ+PLGAG+ FA KY Sbjct: 143 TRGLSIRSIIGELMGRQCGASKGKGGSMHIFAKNFYGGNGIVGAQIPLGAGIGFAQKY 200 Score = 85.4 bits (202), Expect = 7e-18 Identities = 36/57 (63%), Positives = 46/57 (80%) Frame = +1 Query: 514 LPCVFVCENNGYGMGTSVDRSSASTEYYTRGDYVPGVWVDGMDVLATREAARFAIEY 684 LP +F CENN YGMGTS +RSSA TE+Y RG Y+PG+ V+GMDVLA +A++FA +Y Sbjct: 233 LPVIFACENNKYGMGTSAERSSAMTEFYKRGQYIPGLLVNGMDVLAVLQASKFAKKY 289 Score = 52.0 bits (119), Expect = 8e-08 Identities = 23/42 (54%), Positives = 30/42 (71%) Frame = +2 Query: 383 PVGSWSGLRPQVPRRRGVTFALYGDGAANQGQLFEAYNMSKL 508 P+G+ G + + TFALYGDGA+NQGQ FEA+NM+KL Sbjct: 189 PLGAGIGFAQKYLEKPTTTFALYGDGASNQGQAFEAFNMAKL 230 >SPAC23H3.09c |gly1||threonine aldolase |Schizosaccharomyces pombe|chr 1|||Manual Length = 376 Score = 27.5 bits (58), Expect = 1.9 Identities = 10/21 (47%), Positives = 16/21 (76%) Frame = -2 Query: 137 PRMIFSLYRFPDAVSILRNIV 75 PR++F + PDAV IL+N++ Sbjct: 332 PRIVFHIQITPDAVEILKNVL 352 >SPBC839.06 |cta3||P-type ATPase, calcium transporting Cta3|Schizosaccharomyces pombe|chr 2|||Manual Length = 1037 Score = 27.5 bits (58), Expect = 1.9 Identities = 15/53 (28%), Positives = 23/53 (43%) Frame = +3 Query: 336 YGRNFYGGNGIVGAQVPLGAGVAFAHKYRADGESRSLCTETEPPTRVNSSKPT 494 +G + G++ A + L V F +Y+A+ SL T P V S T Sbjct: 79 FGTTDWIEGGVISAIIVLNITVGFIQEYKAEKTMDSLRTLASPMAHVTRSSKT 131 >SPBC336.01 |fbh1|fdh1, fdh|DNA helicase I|Schizosaccharomyces pombe|chr 2|||Manual Length = 878 Score = 27.1 bits (57), Expect = 2.6 Identities = 9/28 (32%), Positives = 16/28 (57%) Frame = +1 Query: 541 NGYGMGTSVDRSSASTEYYTRGDYVPGV 624 NG+ GT + + A EYY++ D + + Sbjct: 82 NGFVNGTQISSTPAEREYYSKSDEIKNI 109 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/33 (39%), Positives = 15/33 (45%) Frame = -3 Query: 397 PAPNGTCAPTIPLPP*KLRPYKCMEPPLPREQP 299 P P A +IPLPP + PP PR P Sbjct: 341 PPPRSNAAGSIPLPP---QGRSAPPPPPPRSAP 370 >SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 1887 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = -3 Query: 139 NHG*SFLYIGFLMRFQSSAISSVVHTA*EHLQ 44 N+G S L I LM + + +V+HTA +H+Q Sbjct: 147 NYGLSILSIHALMCVECECLLNVIHTA-QHMQ 177 >SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = -3 Query: 139 NHG*SFLYIGFLMRFQSSAISSVVHTA*EHLQ 44 N+G S L I LM + + +V+HTA +H+Q Sbjct: 147 NYGLSILSIHALMCVECECLLNVIHTA-QHMQ 177 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,550,798 Number of Sequences: 5004 Number of extensions: 49019 Number of successful extensions: 160 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 146 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 160 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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