Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0657
(693 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alp... 87 1e-17
At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alp... 86 2e-17
At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alp... 69 4e-12
At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putativ... 39 0.004
At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putativ... 38 0.005
At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putativ... 38 0.005
At5g34780.1 68418.m04048 dehydrogenase E1 component family prote... 36 0.034
At5g49220.1 68418.m06093 expressed protein 29 2.2
At3g58510.2 68416.m06522 DEAD box RNA helicase, putative (RH11) ... 29 3.9
At3g58510.1 68416.m06521 DEAD box RNA helicase, putative (RH11) ... 29 3.9
At1g53180.1 68414.m06027 expressed protein 29 3.9
At2g25737.1 68415.m03087 expressed protein contains Pfam profil... 28 5.1
At1g26110.1 68414.m03186 expressed protein 28 5.1
At1g09575.1 68414.m01074 expressed protein contains Pfam domain,... 28 5.1
At1g79730.1 68414.m09300 hydroxyproline-rich glycoprotein family... 28 6.8
>At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial (PDHE1-A) identical to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}
Length = 389
Score = 87.0 bits (206), Expect = 1e-17
Identities = 37/72 (51%), Positives = 53/72 (73%)
Frame = +1
Query: 19 PATSATLTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYSGQEAVAVGMRAAMR 198
P+ S +S++ L + + ++RR+E A+ +LYK K+IRGFCHLY GQEAVA+GM AA+
Sbjct: 48 PSRSVESSSQELLDFFRTMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAIT 107
Query: 199 DADSVITAYRCH 234
D++ITAYR H
Sbjct: 108 KKDAIITAYRDH 119
Score = 85.8 bits (203), Expect = 2e-17
Identities = 40/64 (62%), Positives = 48/64 (75%), Gaps = 2/64 (3%)
Frame = +3
Query: 243 LSHGVSVLGVLSELTGRRTGCSRGKGGSMHLYGR--NFYGGNGIVGAQVPLGAGVAFAHK 416
L G S+ V SEL GR+ GCS+GKGGSMH Y + +FYGG+GIVGAQVPLG G+AFA K
Sbjct: 123 LGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPLGCGIAFAQK 182
Query: 417 YRAD 428
Y +
Sbjct: 183 YNKE 186
Score = 78.6 bits (185), Expect = 4e-15
Identities = 33/58 (56%), Positives = 43/58 (74%)
Frame = +1
Query: 511 DLPCVFVCENNGYGMGTSVDRSSASTEYYTRGDYVPGVWVDGMDVLATREAARFAIEY 684
DLP + VCENN YGMGT+ R++ S YY RGDYVPG+ VDGMD A ++A +FA ++
Sbjct: 215 DLPAILVCENNHYGMGTAEWRAAKSPSYYKRGDYVPGLKVDGMDAFAVKQACKFAKQH 272
Score = 51.2 bits (117), Expect = 6e-07
Identities = 24/42 (57%), Positives = 28/42 (66%)
Frame = +2
Query: 383 PVGSWSGLRPQVPRRRGVTFALYGDGAANQGQLFEAYNMSKL 508
P+G + + VTFALYGDGAANQGQLFEA N+S L
Sbjct: 172 PLGCGIAFAQKYNKEEAVTFALYGDGAANQGQLFEALNISAL 213
>At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alpha
subunit, mitochondrial, putative similar to SP|P52901
Pyruvate dehydrogenase E1 component alpha subunit,
mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
{Arabidopsis thaliana}; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 393
Score = 86.2 bits (204), Expect = 2e-17
Identities = 38/76 (50%), Positives = 54/76 (71%)
Frame = +1
Query: 7 LDQGPATSATLTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYSGQEAVAVGMR 186
L + P+ S +SE+ L + + +RR+E A+ +LYK K+IRGFCHLY GQEA+AVGM
Sbjct: 48 LCESPSRSVETSSEEILAFFRDMARMRRMEIAADSLYKAKLIRGFCHLYDGQEALAVGME 107
Query: 187 AAMRDADSVITAYRCH 234
AA+ D++IT+YR H
Sbjct: 108 AAITKKDAIITSYRDH 123
Score = 85.8 bits (203), Expect = 2e-17
Identities = 38/64 (59%), Positives = 47/64 (73%), Gaps = 2/64 (3%)
Frame = +3
Query: 243 LSHGVSVLGVLSELTGRRTGCSRGKGGSMHLYGRN--FYGGNGIVGAQVPLGAGVAFAHK 416
+ G ++ SEL GR+TGCS GKGGSMH Y ++ FYGG+GIVGAQ+PLG G+AFA K
Sbjct: 127 IGRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPLGCGLAFAQK 186
Query: 417 YRAD 428
Y D
Sbjct: 187 YNKD 190
Score = 81.4 bits (192), Expect = 5e-16
Identities = 35/58 (60%), Positives = 44/58 (75%)
Frame = +1
Query: 511 DLPCVFVCENNGYGMGTSVDRSSASTEYYTRGDYVPGVWVDGMDVLATREAARFAIEY 684
DLP + VCENN YGMGT+ RS+ S Y+ RGDYVPG+ VDGMD LA ++A +FA E+
Sbjct: 219 DLPAILVCENNHYGMGTATWRSAKSPAYFKRGDYVPGLKVDGMDALAVKQACKFAKEH 276
Score = 50.4 bits (115), Expect = 1e-06
Identities = 24/42 (57%), Positives = 28/42 (66%)
Frame = +2
Query: 383 PVGSWSGLRPQVPRRRGVTFALYGDGAANQGQLFEAYNMSKL 508
P+G + + VTFALYGDGAANQGQLFEA N+S L
Sbjct: 176 PLGCGLAFAQKYNKDEAVTFALYGDGAANQGQLFEALNISAL 217
>At1g01090.1 68414.m00011 pyruvate dehydrogenase E1 component alpha
subunit, chloroplast identical to pyruvate dehydrogenase
E1 alpha subunit GB:AAB86803 GI:2454182 from
[Arabidopsis thaliana]; identical to cDNA pyruvate
dehydrogenase E1 alpha subunit mRNA, nuclear gene
encoding plastid protein GI:2454181
Length = 428
Score = 68.5 bits (160), Expect = 4e-12
Identities = 31/64 (48%), Positives = 42/64 (65%), Gaps = 2/64 (3%)
Frame = +3
Query: 243 LSHGVSVLGVLSELTGRRTGCSRGKGGSMHLYGR--NFYGGNGIVGAQVPLGAGVAFAHK 416
LS GVS V+SEL G+ TGC RG+GGSMH++ + N GG +G +P+ G AF+ K
Sbjct: 148 LSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHNMLGGFAFIGEGIPVATGAAFSSK 207
Query: 417 YRAD 428
YR +
Sbjct: 208 YRRE 211
Score = 60.5 bits (140), Expect = 1e-09
Identities = 28/70 (40%), Positives = 41/70 (58%)
Frame = +1
Query: 25 TSATLTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYSGQEAVAVGMRAAMRDA 204
TS +T E+ L+LYE + + R E +Y + GF HLY+GQEAV+ G + +
Sbjct: 75 TSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKS 134
Query: 205 DSVITAYRCH 234
DSV++ YR H
Sbjct: 135 DSVVSTYRDH 144
Score = 50.0 bits (114), Expect = 1e-06
Identities = 26/57 (45%), Positives = 37/57 (64%), Gaps = 2/57 (3%)
Frame = +1
Query: 514 LPCVFVCENNGYGMGTSVDRSSASTEYYTRGDY--VPGVWVDGMDVLATREAARFAI 678
LP +FV ENN + +G S R+++ E + +G +PGV VDGMDVL RE A+ A+
Sbjct: 247 LPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAV 303
Score = 34.3 bits (75), Expect = 0.078
Identities = 14/25 (56%), Positives = 16/25 (64%)
Frame = +2
Query: 434 VTFALYGDGAANQGQLFEAYNMSKL 508
VT A +GDG N GQ FE NM+ L
Sbjct: 220 VTVAFFGDGTCNNGQFFECLNMAAL 244
>At1g21400.1 68414.m02678 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 472
Score = 38.7 bits (86), Expect = 0.004
Identities = 20/59 (33%), Positives = 28/59 (47%), Gaps = 2/59 (3%)
Frame = +1
Query: 511 DLPCVFVCENNGYGMGTSVDRSSASTEYYTRGDY--VPGVWVDGMDVLATREAARFAIE 681
+ P VF+C NNG+ + T + S +G + + VDG D LA A R A E
Sbjct: 285 EAPVVFICRNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSARE 343
>At5g09300.2 68418.m01077 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 401
Score = 38.3 bits (85), Expect = 0.005
Identities = 55/231 (23%), Positives = 92/231 (39%), Gaps = 16/231 (6%)
Frame = +1
Query: 37 LTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYS---GQEAVAVGMRAAMRDAD 207
++ E A+K+Y + L+ ++ N++ E +G Y+ G+EA+ + AA+ D
Sbjct: 54 VSEEVAVKIYSDMVTLQIMD----NIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQD 109
Query: 208 SVITAYRCHGWTYLMGLV-------CWGCSRS*RGAG---PVAPGAREVPCICTDATSMV 357
+ YR G G C+G ++S G G PV G+ ++ AT +
Sbjct: 110 VIFPQYREPGVLLWRGFTLQEFANQCFG-NKSDYGKGRQMPVHYGSNKLNYFTVSAT--I 166
Query: 358 ATGLL-VRRSRWELEWPSPTSTAPTGSHXXXXXXXXXQPGSTLRSLQHV*TRDLPCVFVC 534
AT L + + L+ + A T G +L + P +F+C
Sbjct: 167 ATQLPNAVGAAYSLKMDKKDACAVT-----YFGDGGTSEGDFHAALNIAAVMEAPVLFIC 221
Query: 535 ENNGYGMGTSVDRSSASTEYYTRGDY--VPGVWVDGMDVLATREAARFAIE 681
NNG+ + T S +G + + VDG D LA A A E
Sbjct: 222 RNNGWAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTARE 272
>At5g09300.1 68418.m01078 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 alpha
subunit, putative similar to branched-chain alpha-keto
acid dehydrogenase E1-alpha subunit [Gallus gallus]
GI:12964598; contains Pfam profile PF00676:
Dehydrogenase E1 component
Length = 472
Score = 38.3 bits (85), Expect = 0.005
Identities = 55/231 (23%), Positives = 92/231 (39%), Gaps = 16/231 (6%)
Frame = +1
Query: 37 LTSEDALKLYEQLTILRRIETASGNLYKEKIIRGFCHLYS---GQEAVAVGMRAAMRDAD 207
++ E A+K+Y + L+ ++ N++ E +G Y+ G+EA+ + AA+ D
Sbjct: 125 VSEEVAVKIYSDMVTLQIMD----NIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQD 180
Query: 208 SVITAYRCHGWTYLMGLV-------CWGCSRS*RGAG---PVAPGAREVPCICTDATSMV 357
+ YR G G C+G ++S G G PV G+ ++ AT +
Sbjct: 181 VIFPQYREPGVLLWRGFTLQEFANQCFG-NKSDYGKGRQMPVHYGSNKLNYFTVSAT--I 237
Query: 358 ATGLL-VRRSRWELEWPSPTSTAPTGSHXXXXXXXXXQPGSTLRSLQHV*TRDLPCVFVC 534
AT L + + L+ + A T G +L + P +F+C
Sbjct: 238 ATQLPNAVGAAYSLKMDKKDACAVT-----YFGDGGTSEGDFHAALNIAAVMEAPVLFIC 292
Query: 535 ENNGYGMGTSVDRSSASTEYYTRGDY--VPGVWVDGMDVLATREAARFAIE 681
NNG+ + T S +G + + VDG D LA A A E
Sbjct: 293 RNNGWAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTARE 343
>At5g34780.1 68418.m04048 dehydrogenase E1 component family protein
similar to SP|P50136 2-oxoisovalerate dehydrogenase
alpha subunit, mitochondrial precursor (EC 1.2.4.4)
(Branched-chain alpha-keto acid dehydrogenase component
alpha chain) {Mus musculus}; contains Pfam profile
PF00676: Dehydrogenase E1 component
Length = 365
Score = 35.5 bits (78), Expect = 0.034
Identities = 19/59 (32%), Positives = 27/59 (45%), Gaps = 2/59 (3%)
Frame = +1
Query: 511 DLPCVFVCENNGYGMGTSVDRSSASTEYYTRGDY--VPGVWVDGMDVLATREAARFAIE 681
+ P VF+C NNG+ + T + S +G + + VDG D LA A A E
Sbjct: 55 EAPVVFICRNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVCSARE 113
>At5g49220.1 68418.m06093 expressed protein
Length = 409
Score = 29.5 bits (63), Expect = 2.2
Identities = 17/47 (36%), Positives = 21/47 (44%)
Frame = +1
Query: 259 VCWGCSRS*RGAGPVAPGAREVPCICTDATSMVATGLLVRRSRWELE 399
VC G S S G+G V + T +V L RSRWEL+
Sbjct: 88 VCAGSSDSSSGSGRVLSDGSNLDRFLEHTTPVVPARLFPMRSRWELK 134
>At3g58510.2 68416.m06522 DEAD box RNA helicase, putative (RH11)
similar to RNA helicase DBY protein [Mus musculus]
GI:3790186, SP|O00571 DEAD-box protein 3 (Helicase-like
protein 2) {Homo sapiens}; contains Pfam profiles
PF00270: DEAD/DEAH box helicase, PF00271: Helicase
conserved C-terminal domain; identical to cDNA DEAD box
RNA helicase, RH11 GI:3775998
Length = 612
Score = 28.7 bits (61), Expect = 3.9
Identities = 18/40 (45%), Positives = 18/40 (45%), Gaps = 1/40 (2%)
Frame = +3
Query: 294 RTGCSRGKGGSMHLYGRNFYGGNGIVGA-QVPLGAGVAFA 410
R G G GG YG YGG G GA GAGV A
Sbjct: 571 RGGGGGGGGGGSDYYGGGGYGGGGYGGAPSGGYGAGVTSA 610
>At3g58510.1 68416.m06521 DEAD box RNA helicase, putative (RH11)
similar to RNA helicase DBY protein [Mus musculus]
GI:3790186, SP|O00571 DEAD-box protein 3 (Helicase-like
protein 2) {Homo sapiens}; contains Pfam profiles
PF00270: DEAD/DEAH box helicase, PF00271: Helicase
conserved C-terminal domain; identical to cDNA DEAD box
RNA helicase, RH11 GI:3775998
Length = 612
Score = 28.7 bits (61), Expect = 3.9
Identities = 18/40 (45%), Positives = 18/40 (45%), Gaps = 1/40 (2%)
Frame = +3
Query: 294 RTGCSRGKGGSMHLYGRNFYGGNGIVGA-QVPLGAGVAFA 410
R G G GG YG YGG G GA GAGV A
Sbjct: 571 RGGGGGGGGGGSDYYGGGGYGGGGYGGAPSGGYGAGVTSA 610
>At1g53180.1 68414.m06027 expressed protein
Length = 358
Score = 28.7 bits (61), Expect = 3.9
Identities = 22/54 (40%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = +3
Query: 135 WFLSPVFRTRSRGSRYESSDARCRLSNHRVSLSRMDLSHGVSVLG-VLSELTGR 293
WF +F RSRG RY SD S+ S +M G S +G VL TGR
Sbjct: 19 WFFENLFTRRSRGLRYCHSDPYPSSSSTSTSPEKM----GDSDIGKVLEASTGR 68
>At2g25737.1 68415.m03087 expressed protein contains Pfam profile:
PF01925 domain of unknown function DUF81
Length = 476
Score = 28.3 bits (60), Expect = 5.1
Identities = 22/73 (30%), Positives = 36/73 (49%), Gaps = 5/73 (6%)
Frame = +3
Query: 195 ARCRLSNHRVSLSRMDLSHGVSVLGVLSELTGRRTGCSRGKGGSMHLYGR-----NFYGG 359
A C ++ ++L ++ ++ GVS ++ GRR S+G+G S G+ F
Sbjct: 283 ANCSVAYWVINLLQIPVAVGVSGYEAVALYQGRRIIASKGQGDSNFTVGQLVMYCTFGII 342
Query: 360 NGIVGAQVPLGAG 398
GIVG + LG G
Sbjct: 343 AGIVGGLLGLGGG 355
>At1g26110.1 68414.m03186 expressed protein
Length = 611
Score = 28.3 bits (60), Expect = 5.1
Identities = 27/87 (31%), Positives = 34/87 (39%)
Frame = +3
Query: 183 ESSDARCRLSNHRVSLSRMDLSHGVSVLGVLSELTGRRTGCSRGKGGSMHLYGRNFYGGN 362
ES ++R R S R ++D G S G R G RG G + Y R YGG
Sbjct: 531 ESQNSRPRFSEQR----KLD----TETFGEFSRFRGGRGG--RGGYGRNNGYSRGGYGGR 580
Query: 363 GIVGAQVPLGAGVAFAHKYRADGESRS 443
G G G G + + R G S
Sbjct: 581 GYGGYGGRGGGGGGYGYGGRGQGRGVS 607
>At1g09575.1 68414.m01074 expressed protein contains Pfam domain,
PF04678: Protein of unknown function, DUF607
Length = 292
Score = 28.3 bits (60), Expect = 5.1
Identities = 12/23 (52%), Positives = 13/23 (56%)
Frame = +1
Query: 334 CTDATSMVATGLLVRRSRWELEW 402
C ATSMV GL R + WE W
Sbjct: 175 CGLATSMVQIGLFFRLTFWEFSW 197
>At1g79730.1 68414.m09300 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 589
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/31 (38%), Positives = 15/31 (48%)
Frame = -3
Query: 403 ATPAPNGTCAPTIPLPP*KLRPYKCMEPPLP 311
A P P + P +P PP +PY PP P
Sbjct: 21 APPPPPPSLPPPVPPPPPSHQPYSYPPPPPP 51
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,207,505
Number of Sequences: 28952
Number of extensions: 296802
Number of successful extensions: 867
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 809
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 862
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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