Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= mg--0652
(714 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1672.09 |||triglyceride lipase-cholesterol esterase |Schizos... 74 2e-14
SPBC16A3.12c |||triglyceride lipase-cholesterol esterase |Schizo... 72 7e-14
SPBC14C8.15 |||triglyceride lipase-cholesterol esterase |Schizos... 66 4e-12
SPCC1020.13c ||SPCC14G10.05|phospholipase |Schizosaccharomyces p... 31 0.16
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 29 0.66
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 28 1.5
SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|c... 26 4.7
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 26 4.7
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.1
SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ... 25 8.1
>SPCC1672.09 |||triglyceride lipase-cholesterol esterase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 467
Score = 73.7 bits (173), Expect = 2e-14
Identities = 38/85 (44%), Positives = 47/85 (55%), Gaps = 2/85 (2%)
Frame = +1
Query: 259 PDPKK-PAVLVMHGLFASAADWVL-MGPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKL 432
P PKK P V HGL ++ WV + P L + L GYDVWLGN RG YSR H++
Sbjct: 121 PHPKKLPVVYCHHGLLMNSEVWVCNVDPRNCLVFDLVNKGYDVWLGNNRGNKYSRQHLRF 180
Query: 433 DPDNDSEFWKFSWEEIGTRDLPAMI 507
D D EFW FS ++ D+P I
Sbjct: 181 D-STDKEFWDFSIDDFAQYDIPDTI 204
Score = 41.5 bits (93), Expect = 1e-04
Identities = 21/45 (46%), Positives = 28/45 (62%)
Frame = +3
Query: 510 YTLKVAGKRRLHYIGHSQGTTVFWAMGSLRPEYNSKIIAMQAYAP 644
Y LK +G+ +L YIG SQGT +A S+ P N KI ++ A AP
Sbjct: 206 YILKTSGQTKLTYIGFSQGTAQAFASLSIHPLLNDKINSLIALAP 250
Score = 27.1 bits (57), Expect = 2.7
Identities = 16/31 (51%), Positives = 18/31 (58%)
Frame = +2
Query: 140 DIVGLIKKYRYPVEVHKVVTEDGYILEMQRI 232
DI + K Y VE H V TED YIL + RI
Sbjct: 80 DIREMCKISGYYVEDHLVRTEDDYILCIHRI 110
>SPBC16A3.12c |||triglyceride lipase-cholesterol esterase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 443
Score = 72.1 bits (169), Expect = 7e-14
Identities = 34/78 (43%), Positives = 45/78 (57%), Gaps = 1/78 (1%)
Frame = +1
Query: 268 KKPAVLVMHGLFASAADWVLMGPGV-GLAYILAEAGYDVWLGNARGTYYSRAHIKLDPDN 444
K+ V HGL ++ WV + L ++L E+GYDVWLGN RG YSR HI P
Sbjct: 113 KREVVYCHHGLMTNSELWVAVNESERSLPFVLIESGYDVWLGNNRGNKYSRKHITYKP-K 171
Query: 445 DSEFWKFSWEEIGTRDLP 498
D EFW FS +++ D+P
Sbjct: 172 DEEFWNFSLDDMAMFDIP 189
Score = 40.7 bits (91), Expect = 2e-04
Identities = 22/63 (34%), Positives = 36/63 (57%), Gaps = 4/63 (6%)
Frame = +3
Query: 510 YTLKVAGKRRLHYIGHSQGTTVFWAMGSLRPEYNSKI---IAM-QAYAPVAYLEFNANRL 677
Y L+ G+ +L+YIG SQGT A S+ P+ N K+ I + AYAP + + + +
Sbjct: 194 YILRETGREKLNYIGFSQGTAQAMAALSINPDLNDKVNIFIGLAPAYAPKGFSNYFVDYI 253
Query: 678 LKL 686
+K+
Sbjct: 254 VKV 256
>SPBC14C8.15 |||triglyceride lipase-cholesterol esterase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 66.5 bits (155), Expect = 4e-12
Identities = 32/79 (40%), Positives = 45/79 (56%), Gaps = 1/79 (1%)
Frame = +1
Query: 274 PAVLVMHGLFASAADWVL-MGPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKLDPDNDS 450
P VL +HGL ++ WV + + + L E GYDVWLGN RG YS +IK N
Sbjct: 119 PPVLFIHGLMMNSESWVCNLKKEDAIPFALVEQGYDVWLGNLRGNKYSIKNIKFSSQN-P 177
Query: 451 EFWKFSWEEIGTRDLPAMI 507
+FW FS + I D+P+++
Sbjct: 178 KFWDFSLDSIAIFDIPSIV 196
>SPCC1020.13c ||SPCC14G10.05|phospholipase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 669
Score = 31.1 bits (67), Expect = 0.16
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = +3
Query: 504 DCYTLKVAGKRRLHYIGHSQGTTVFWAMGSLRPEYNSKI 620
DC + K R+ IGHS G+TV + + SL+P + +I
Sbjct: 382 DCNPYFLENKGRIFIIGHSLGSTVVFDILSLQPTFVKEI 420
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 29.1 bits (62), Expect = 0.66
Identities = 23/71 (32%), Positives = 35/71 (49%)
Frame = -2
Query: 641 SISLHSNNFAVVFRSQRSHSPKDSSSL*VTNIMKSSFSSNFQSVTIIAGRSRVPISSQLN 462
S S S++ + S S S SSS ++ SS SS+ S + + S VPI+S +
Sbjct: 156 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTS 215
Query: 461 FQNSESLSGSN 429
+S S S S+
Sbjct: 216 SSHSSSSSSSS 226
>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 242
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/43 (27%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +3
Query: 540 LHYIGHSQGTTVFWAMGSLRPEYNSKII-AMQAYAPVAYLEFN 665
LH++ +S+ T + W + L+ Y K+ + AP AY + +
Sbjct: 4 LHHLKNSRSTRIVWMLEELKVPYEIKVYDRVDGRAPPAYTKLS 46
>SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 521
Score = 26.2 bits (55), Expect = 4.7
Identities = 15/36 (41%), Positives = 17/36 (47%)
Frame = +1
Query: 169 ISSRSSQGRYGRWLYLRNAKNSSWTRPEHRPDPKKP 276
+ SR G G +LRNAK S P RP K P
Sbjct: 385 LGSRMLNGLGGSADFLRNAKLSIMHTPSVRPSKKDP 420
>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 817
Score = 26.2 bits (55), Expect = 4.7
Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 1/51 (1%)
Frame = -2
Query: 602 RSQRSHSPKDSSSL*VTNIMKSSFSSNFQSVTIIAGRSRVPI-SSQLNFQN 453
+S SH P+ SSSL + + F+SN TI S I +L+ QN
Sbjct: 7 KSLTSHLPQSSSSLSQSREIAKEFTSNIPPPTIKTNSSSSNILKPRLSLQN 57
>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 536
Score = 25.8 bits (54), Expect = 6.1
Identities = 24/72 (33%), Positives = 36/72 (50%)
Frame = -2
Query: 650 SYGSISLHSNNFAVVFRSQRSHSPKDSSSL*VTNIMKSSFSSNFQSVTIIAGRSRVPISS 471
S S +L S++ A S SH+ SSS +++ SS SS S + ++ S VP SS
Sbjct: 100 SSSSATLTSSSSASPTSSSSSHALSSSSS----SLVASSSSSGMSS-SSLSHSSSVPSSS 154
Query: 470 QLNFQNSESLSG 435
+S + SG
Sbjct: 155 SSYHSSSMTTSG 166
>SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 582
Score = 25.4 bits (53), Expect = 8.1
Identities = 18/57 (31%), Positives = 22/57 (38%)
Frame = +2
Query: 92 AQGARYSTNVTEDALLDIVGLIKKYRYPVEVHKVVTEDGYILEMQRIPHGRDQNTVL 262
+QG YSTN DA DI Y E Y + PH + QN +L
Sbjct: 198 SQGYMYSTNTAHDA-TDIPSSFNFYNTQASTAPNPQEINYQWSHEYRPHTQYQNNLL 253
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,103,411
Number of Sequences: 5004
Number of extensions: 66729
Number of successful extensions: 194
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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