Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= mg--0651
         (837 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1672.09 |||triglyceride lipase-cholesterol esterase |Schizos...    74   3e-14
SPBC16A3.12c |||triglyceride lipase-cholesterol esterase |Schizo...    72   9e-14
SPBC14C8.15 |||triglyceride lipase-cholesterol esterase |Schizos...    66   4e-12
SPCC1020.13c ||SPCC14G10.05|phospholipase |Schizosaccharomyces p...    31   0.20 
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi...    29   0.82 
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce...    28   1.9  
SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|c...    26   5.8  
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ...    26   5.8  
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   7.6  

>SPCC1672.09 |||triglyceride lipase-cholesterol esterase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 467

 Score = 73.7 bits (173), Expect = 3e-14
 Identities = 38/85 (44%), Positives = 47/85 (55%), Gaps = 2/85 (2%)
 Frame = +1

Query: 259 PDPKK-PAVLVMHGLFASAADWVL-MGPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKL 432
           P PKK P V   HGL  ++  WV  + P   L + L   GYDVWLGN RG  YSR H++ 
Sbjct: 121 PHPKKLPVVYCHHGLLMNSEVWVCNVDPRNCLVFDLVNKGYDVWLGNNRGNKYSRQHLRF 180

Query: 433 DPDNDSEFWKFSWEEIGTRDLPAMI 507
           D   D EFW FS ++    D+P  I
Sbjct: 181 D-STDKEFWDFSIDDFAQYDIPDTI 204



 Score = 43.6 bits (98), Expect = 4e-05
 Identities = 31/88 (35%), Positives = 46/88 (52%)
 Frame = +3

Query: 510 YTLKVAGKRRLHYIGHSQGTTVFWAMGSLRPEYNSKIIAMQAYAPVAYLEFNANRLLKLI 689
           Y LK +G+ +L YIG SQGT   +A  S+ P  N KI ++ A AP    +   NR++   
Sbjct: 206 YILKTSGQTKLTYIGFSQGTAQAFASLSIHPLLNDKINSLIALAPAISPKGLHNRVVDAF 265

Query: 690 APHANSIEALTSLIGINELFGRSDSLPT 773
                 ++A  S++    LFGR   LP+
Sbjct: 266 ------VKARPSILFF--LFGRKSILPS 285



 Score = 27.1 bits (57), Expect = 3.3
 Identities = 16/31 (51%), Positives = 18/31 (58%)
 Frame = +2

Query: 140 DIVGLIKKYRYPVEVHKVVTEDGYILEMQRI 232
           DI  + K   Y VE H V TED YIL + RI
Sbjct: 80  DIREMCKISGYYVEDHLVRTEDDYILCIHRI 110


>SPBC16A3.12c |||triglyceride lipase-cholesterol esterase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 443

 Score = 72.1 bits (169), Expect = 9e-14
 Identities = 34/78 (43%), Positives = 45/78 (57%), Gaps = 1/78 (1%)
 Frame = +1

Query: 268 KKPAVLVMHGLFASAADWVLMGPGV-GLAYILAEAGYDVWLGNARGTYYSRAHIKLDPDN 444
           K+  V   HGL  ++  WV +      L ++L E+GYDVWLGN RG  YSR HI   P  
Sbjct: 113 KREVVYCHHGLMTNSELWVAVNESERSLPFVLIESGYDVWLGNNRGNKYSRKHITYKP-K 171

Query: 445 DSEFWKFSWEEIGTRDLP 498
           D EFW FS +++   D+P
Sbjct: 172 DEEFWNFSLDDMAMFDIP 189



 Score = 40.7 bits (91), Expect = 3e-04
 Identities = 22/63 (34%), Positives = 36/63 (57%), Gaps = 4/63 (6%)
 Frame = +3

Query: 510 YTLKVAGKRRLHYIGHSQGTTVFWAMGSLRPEYNSKI---IAM-QAYAPVAYLEFNANRL 677
           Y L+  G+ +L+YIG SQGT    A  S+ P+ N K+   I +  AYAP  +  +  + +
Sbjct: 194 YILRETGREKLNYIGFSQGTAQAMAALSINPDLNDKVNIFIGLAPAYAPKGFSNYFVDYI 253

Query: 678 LKL 686
           +K+
Sbjct: 254 VKV 256


>SPBC14C8.15 |||triglyceride lipase-cholesterol esterase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score = 66.5 bits (155), Expect = 4e-12
 Identities = 32/79 (40%), Positives = 45/79 (56%), Gaps = 1/79 (1%)
 Frame = +1

Query: 274 PAVLVMHGLFASAADWVL-MGPGVGLAYILAEAGYDVWLGNARGTYYSRAHIKLDPDNDS 450
           P VL +HGL  ++  WV  +     + + L E GYDVWLGN RG  YS  +IK    N  
Sbjct: 119 PPVLFIHGLMMNSESWVCNLKKEDAIPFALVEQGYDVWLGNLRGNKYSIKNIKFSSQN-P 177

Query: 451 EFWKFSWEEIGTRDLPAMI 507
           +FW FS + I   D+P+++
Sbjct: 178 KFWDFSLDSIAIFDIPSIV 196


>SPCC1020.13c ||SPCC14G10.05|phospholipase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 669

 Score = 31.1 bits (67), Expect = 0.20
 Identities = 15/39 (38%), Positives = 23/39 (58%)
 Frame = +3

Query: 504 DCYTLKVAGKRRLHYIGHSQGTTVFWAMGSLRPEYNSKI 620
           DC    +  K R+  IGHS G+TV + + SL+P +  +I
Sbjct: 382 DCNPYFLENKGRIFIIGHSLGSTVVFDILSLQPTFVKEI 420


>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 374

 Score = 29.1 bits (62), Expect = 0.82
 Identities = 23/71 (32%), Positives = 35/71 (49%)
 Frame = -2

Query: 641 SISLHSNNFAVVFRSQRSHSPKDSSSL*VTNIMKSSFSSNFQSVTIIAGRSRVPISSQLN 462
           S S  S++ +    S  S S   SSS   ++   SS SS+  S +  +  S VPI+S  +
Sbjct: 156 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTS 215

Query: 461 FQNSESLSGSN 429
             +S S S S+
Sbjct: 216 SSHSSSSSSSS 226


>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 242

 Score = 27.9 bits (59), Expect = 1.9
 Identities = 12/43 (27%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
 Frame = +3

Query: 540 LHYIGHSQGTTVFWAMGSLRPEYNSKII-AMQAYAPVAYLEFN 665
           LH++ +S+ T + W +  L+  Y  K+   +   AP AY + +
Sbjct: 4   LHHLKNSRSTRIVWMLEELKVPYEIKVYDRVDGRAPPAYTKLS 46


>SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 521

 Score = 26.2 bits (55), Expect = 5.8
 Identities = 15/36 (41%), Positives = 17/36 (47%)
 Frame = +1

Query: 169 ISSRSSQGRYGRWLYLRNAKNSSWTRPEHRPDPKKP 276
           + SR   G  G   +LRNAK S    P  RP  K P
Sbjct: 385 LGSRMLNGLGGSADFLRNAKLSIMHTPSVRPSKKDP 420


>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 817

 Score = 26.2 bits (55), Expect = 5.8
 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 1/51 (1%)
 Frame = -2

Query: 602 RSQRSHSPKDSSSL*VTNIMKSSFSSNFQSVTIIAGRSRVPI-SSQLNFQN 453
           +S  SH P+ SSSL  +  +   F+SN    TI    S   I   +L+ QN
Sbjct: 7   KSLTSHLPQSSSSLSQSREIAKEFTSNIPPPTIKTNSSSSNILKPRLSLQN 57


>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 536

 Score = 25.8 bits (54), Expect = 7.6
 Identities = 24/72 (33%), Positives = 36/72 (50%)
 Frame = -2

Query: 650 SYGSISLHSNNFAVVFRSQRSHSPKDSSSL*VTNIMKSSFSSNFQSVTIIAGRSRVPISS 471
           S  S +L S++ A    S  SH+   SSS    +++ SS SS   S + ++  S VP SS
Sbjct: 100 SSSSATLTSSSSASPTSSSSSHALSSSSS----SLVASSSSSGMSS-SSLSHSSSVPSSS 154

Query: 470 QLNFQNSESLSG 435
                +S + SG
Sbjct: 155 SSYHSSSMTTSG 166


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,663,647
Number of Sequences: 5004
Number of extensions: 79180
Number of successful extensions: 221
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 412451140
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)